| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56394SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97931SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56369SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82838-25ul | |||
![Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798] - Western blot analysis of extracts of various cell lines, using SMC3 Rabbit mAb (NBP3-15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Western-Blot-NBP3-15798-img0004.jpg "Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798] - Western blot analysis of extracts of various cell lines, using SMC3 Rabbit mAb (NBP3-15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of C6 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of U2OS using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of NIH/3T3 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0002.jpg)
![Immunoprecipitation: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunoprecipitation analysis of 300ug extracts of U-87MG cells using 3ug SMC3 antibody (NBP3-15798). Western blot was performed from the immunoprecipitate using SMC3 antibody (NBP3-15798) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunoprecipitation-NBP3-15798-img0005.jpg)
![Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-375-img0005.jpg "Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.")
![Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0002.jpg "Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.")
![Flow Cytometry: SMC4 Antibody [NB100-56394] - Western blot analysis of CAP-C in Ramos cell lysate using NB100-56394 at 2 ug/ml.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0001.jpg)
![Immunohistochemistry: PIF1 Antibody [NBP1-97931] - IHC analysis of PIF1 in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PIF1-Antibody-Immunohistochemistry-NBP1-97931-img0001.jpg "Immunohistochemistry: PIF1 Antibody [NBP1-97931] - IHC analysis of PIF1 in mouse brain using DAB with hematoxylin counterstain.")
![Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0001.jpg "Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.")
![Western Blot: ANP32B Antibody [NB100-56369] - analysis of APRIL in HL-60 whole cell lysate using APRIL antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0002.jpg)
![Western Blot: SMC4 Antibody (9Z8S3) [NBP3-16219] - Western blot analysis of extracts of various cell lines, using SMC4 Rabbit mAb (NBP3-16219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SMC4-Antibody-9Z8S3-Western-Blot-NBP3-16219-img0004.jpg "Western Blot: SMC4 Antibody (9Z8S3) [NBP3-16219] - Western blot analysis of extracts of various cell lines, using SMC4 Rabbit mAb (NBP3-16219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219] - Immunofluorescence analysis of U-2 OS cells using SMC4 Rabbit mAb (NBP3-16219) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC4-Antibody-9Z8S3-Immunocytochemistry-Immunofluorescence-NBP3-16219-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219] - Immunofluorescence analysis of NIH/3T3 cells using SMC4 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16219_rabbit-smc4-mab-9z8s3-72202512443316.jpg)
![Immunocytochemistry/ Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219] - Immunofluorescence analysis of C6 cells using SMC4 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16219_rabbit-smc4-mab-9z8s3-72202512435323.jpg)
![Western Blot: SMC4 Antibody [NB100-374] - Detection of Human SMC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC4 antibody NB100-374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-374-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC4 Antibody [NB100-374] - Detection of Human SMC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC4 antibody NB100-374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NB100-374] - HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody used at 5 ug/ml. Detection: Rhodamine labeled anti-rabbit secondary antibody. Cells were counterstained with DAPI.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-374-img0002.jpg)
![Western Blot: SMC4 Antibody [NB100-374] - Whole cell lysate (90 ug) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibody used at 0.2 ug/ml and goat anti-rabbit HRP used at a dilution of 1:20,000.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-374-img0003.jpg)
![Immunohistochemistry-Paraffin: DMC1 Antibody [NBP1-82838] - Analysis in human testis and pancreas tissues using NBP1-82838 antibody. Corresponding DMC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82838-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DMC1 Antibody [NBP1-82838] - Analysis in human testis and pancreas tissues using NBP1-82838 antibody. Corresponding DMC1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DMC1 Antibody [NBP1-82838] - Staining of human duodenum shows no expression in glandular cells as expected.](http://images.novusbio.com/fullsize/DMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82838-img0014.jpg)
![Immunohistochemistry-Paraffin: DMC1 Antibody [NBP1-82838] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferious ducts.](http://images.novusbio.com/fullsize/DMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82838-img0011.jpg)
![Immunohistochemistry-Paraffin: DMC1 Antibody [NBP1-82838] - Staining of human pancreas shows no expression in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/DMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82838-img0012.jpg)
![Immunohistochemistry-Paraffin: DMC1 Antibody [NBP1-82838] - Staining of human liver shows no expression in hepatocytes as expected.](http://images.novusbio.com/fullsize/DMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-82838-img0013.jpg)
![Western Blot: SMC2 Antibody [NBP2-49655] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NBP2-49655-img0003.jpg "Western Blot: SMC2 Antibody [NBP2-49655] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NBP2-49655] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleus.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49655-img0004.jpg)
![Immunohistochemistry-Paraffin: SMC2 Antibody [NBP2-49655] - Staining of human tonsil shows nuclear positivity in germinal center and non germinal center.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunohistochemistry-Paraffin-NBP2-49655-img0002.jpg)
![Western Blot: SMC2 Antibody (PSH0-63) [NBP3-32959] - Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: HT-29 cell lysate (30 ug/Lane) Lane 4: HepG2 cell lysate (30 ug/Lane) Lane 5: A431 cell lysate (30 ug/Lane) Lane 6: SH-SY5Y cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: Raji cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (24 ug/Lane) Predicted band size: 136 kDa Observed band size: 136 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32959) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32959_rabbit-smc2-mab-psh0-63-288202412493484.jpg "Western Blot: SMC2 Antibody (PSH0-63) [NBP3-32959] - Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: HT-29 cell lysate (30 ug/Lane) Lane 4: HepG2 cell lysate (30 ug/Lane) Lane 5: A431 cell lysate (30 ug/Lane) Lane 6: SH-SY5Y cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: Raji cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (24 ug/Lane) Predicted band size: 136 kDa Observed band size: 136 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32959) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SMC2 Antibody (PSH0-63) [NBP3-32959] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32959) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32959_rabbit-smc2-mab-psh0-63-298202414262633.jpg)
![Immunocytochemistry/ Immunofluorescence: SMC2 Antibody (PSH0-63) [NBP3-32959] - Immunocytochemistry analysis of HeLa cells labeling SMC2 with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32959_rabbit-smc2-mab-psh0-63-29202410334025.jpg)
![Western Blot: SMC2 Antibody (PSH0-62) [NBP3-32958] - Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (NBP3-32958) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: HT-29 cell lysate (30 ug/Lane) Lane 4: HepG2 cell lysate (30 ug/Lane) Lane 5: A431 cell lysate (30 ug/Lane) Lane 6: SH-SY5Y cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: Raji cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (24 ug/Lane) Predicted band size: 136 kDa Observed band size: 136 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32958) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32958_rabbit-smc2-mab-psh0-62-288202412442988.jpg "Western Blot: SMC2 Antibody (PSH0-62) [NBP3-32958] - Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (NBP3-32958) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: HT-29 cell lysate (30 ug/Lane) Lane 4: HepG2 cell lysate (30 ug/Lane) Lane 5: A431 cell lysate (30 ug/Lane) Lane 6: SH-SY5Y cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: Raji cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (24 ug/Lane) Predicted band size: 136 kDa Observed band size: 136 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32958) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SMC2 Antibody (PSH0-62) [NBP3-32958] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMC2 antibody (NBP3-32958) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32958) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32958_rabbit-smc2-mab-psh0-62-29820241420911.jpg)
![Immunocytochemistry/ Immunofluorescence: SMC2 Antibody (PSH0-62) [NBP3-32958] - Immunocytochemistry analysis of HeLa cells labeling SMC2 with Rabbit anti-SMC2 antibody (NBP3-32958) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (NBP3-32958) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32958_rabbit-smc2-mab-psh0-62-29202410334030.jpg)
![Western Blot: BRAF35 Antibody [NBP2-15608] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/BRAF35-Antibody-Western-Blot-NBP2-15608-img0001.jpg "Western Blot: BRAF35 Antibody [NBP2-15608] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: BRAF35 Antibody [NBP2-15608] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BRAF35-Antibody-Immunohistochemistry-Paraffin-NBP2-15608-img0002.jpg)
![Immunoprecipitation: BRAF35 Antibody [NBP2-15608] - 293T whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of BRAF35 by 3 ug of BRAF35 antibody 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/BRAF35-Antibody-Immunoprecipitation-NBP2-15608-img0004.jpg)