| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71351 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85804-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71376 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24762SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37928-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70884 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83904-25ul | |||
![Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Western-Blot-NBP2-22527-img0006.jpg "Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunocytochemistry-Immunofluorescence-NBP2-22527-img0003.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human colon carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0005.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0004.jpg)
![Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunoprecipitation-NBP2-22527-img0002.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Western-Blot-NBP1-47757-img0019.jpg "Western Blot: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.")
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Carcinoma of bladder tissue.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0020.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0011.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0012.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0013.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0014.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded endometrium tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0015.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Kidney tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0016.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0017.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded prostate tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Lck Antibody [NBP1-85804] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Lck-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85804-img0005.jpg "Immunocytochemistry/Immunofluorescence: Lck Antibody [NBP1-85804] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/nbp1-85804_rabbit-polyclonal-lck-antibody-2442025930833.jpg)
![Western Blot: GAS7 Antibody (2F6) [NBP2-03061] Analysis of extracts (35ug) from 9 different cell lines by using anti-GAS7 monoclonal antibody.](http://images.novusbio.com/fullsize/GAS7-Antibody-2F6-Western-Blot-NBP2-03061-img0006.jpg "Western Blot: GAS7 Antibody (2F6) [NBP2-03061] Analysis of extracts (35ug) from 9 different cell lines by using anti-GAS7 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: GAS7 Antibody (2F6) [NBP2-03061] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GAS7.](http://images.novusbio.com/fullsize/GAS7-Antibody-2F6-Immunocytochemistry-Immunofluorescence-NBP2-03061-img0005.jpg)
![Immunohistochemistry-Paraffin: GAS7 Antibody (2F6) [NBP2-03061] - Staining of paraffin-embedded Human Kidney tissue using anti-GAS7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GAS7-Antibody-2F6-Immunohistochemistry-Paraffin-NBP2-03061-img0004.jpg)
![Flow Cytometry: GAS7 Antibody (2F6) [NBP2-03061] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GAS7 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/GAS7-Antibody-2F6-Flow-Cytometry-NBP2-03061-img0001.jpg)
![Western Blot: GAS7 Antibody (2F6) [NBP2-03061] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAS7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7.](http://images.novusbio.com/fullsize/GAS7-Antibody-2F6-Western-Blot-NBP2-03061-img0002.jpg)
![Immunohistochemistry-Paraffin: GAS7 Antibody (2F6) [NBP2-03061] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GAS7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GAS7-Antibody-2F6-Immunohistochemistry-Paraffin-NBP2-03061-img0003.jpg)
![Western Blot: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAS7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7.](http://images.novusbio.com/fullsize/GAS7-Antibody-OTI2F6-Azide-and-BSA-Free-Western-Blot-NBP2-71376-img0002.jpg "Western Blot: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAS7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7.")
![Immunocytochemistry/Immunofluorescence: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GAS7.](http://images.novusbio.com/fullsize/GAS7-Antibody-OTI2F6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71376-img0004.jpg)
![Immunohistochemistry: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GAS7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GAS7-Antibody-OTI2F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71376-img0005.jpg)
![Flow Cytometry: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GAS7 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/GAS7-Antibody-OTI2F6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71376-img0003.jpg)
![Western Blot: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GAS7 monoclonal antibody.](http://images.novusbio.com/fullsize/GAS7-Antibody-OTI2F6-Azide-and-BSA-Free-Western-Blot-NBP2-71376-img0001.jpg)
![Immunohistochemistry: GAS7 Antibody (OTI2F6) - Azide and BSA Free [NBP2-71376] - Staining of paraffin-embedded Human Kidney tissue using anti-GAS7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GAS7-Antibody-OTI2F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71376-img0006.jpg)
![Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Human neuroblastoma with various drugs and control. Image from verified customer review.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Western-Blot-NBP2-24762-img0007.jpg "Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Human neuroblastoma with various drugs and control. Image from verified customer review.")
![Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP2-24762] - Human liver tissue using an isotype control (top left) and NBP2-24762 (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Immunohistochemistry-Paraffin-NBP2-24762-img0006.jpg)
![Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Sphingolipid Receptor Edg3/S1P3 in kidney lysate. Lane 1 shows pre-immune sera. Lanes 2 and 3 show NBP2-24762 tested on human kidney (2 ug/ml) and mouse kidney (1 ug/ml) lysate.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Western-Blot-NBP2-24762-img0005.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Western-Blot-NBP2-71351-img0001.jpg "Western Blot: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.")
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0009.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Carcinoma of bladder tissue.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0002.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0003.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0004.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0005.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded endometrium tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0006.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Kidney tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0007.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0008.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded prostate tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0010.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining in human lymph node and cerebral cortex tissues using anti-CCL21 antibody. Corresponding CCL21 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining in human lymph node and cerebral cortex tissues using anti-CCL21 antibody. Corresponding CCL21 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0010.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0004.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0005.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0008.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human placenta shows no cytoplasmic positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0009.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33454-img0003.jpg "Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Rat cervix. 14-3-3 beta antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-33454-img0005.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33454_rabbit-polyclonal-14-3-3-beta-alpha-antibody-western-blot-2611202518274621.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000 and competitor's antibody (sc-628) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33454_rabbit-polyclonal-14-3-3-beta-alpha-antibody-western-blot-2611202518431912.jpg)
![Western Blot: HGF Antibody (1D2) [NBP2-45972] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGF.](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Western-Blot-NBP2-45972-img0007.jpg "Western Blot: HGF Antibody (1D2) [NBP2-45972] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGF.")
![Immunohistochemistry: HGF Antibody (1D2) [NBP2-45972] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0006.jpg)
![Immunohistochemistry: HGF Antibody (OTI1D2) [NBP2-45972] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0001.jpg)
![Immunohistochemistry: HGF Antibody (1D2) [NBP2-45972] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0002.jpg)
![Immunohistochemistry: HGF Antibody (1D2) [NBP2-45972] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0003.jpg)
![Immunohistochemistry: HGF Antibody (1D2) [NBP2-45972] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0004.jpg)
![Immunohistochemistry: HGF Antibody (1D2) [NBP2-45972] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/HGF-Antibody-1D2-Immunohistochemistry-NBP2-45972-img0005.jpg)
![Immunohistochemistry-Paraffin: FGF-BP Antibody [NBP1-83904] - Analysis in human esophagus and liver tissues. Corresponding FGF-BP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FGF-BP-Antibody-Immunohistochemistry-NBP1-83904-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FGF-BP Antibody [NBP1-83904] - Analysis in human esophagus and liver tissues. Corresponding FGF-BP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FGF-BP Antibody [NBP1-83904] - Staining of human tonsil shows moderate to strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/FGF-BP-Antibody-Immunohistochemistry-Paraffin-NBP1-83904-img0015.jpg)
![Immunohistochemistry-Paraffin: FGF-BP Antibody [NBP1-83904] - Staining of human esophagus shows high expression.](http://images.novusbio.com/fullsize/FGF-BP-Antibody-Immunohistochemistry-Paraffin-NBP1-83904-img0007.jpg)
![Immunohistochemistry-Paraffin: FGF-BP Antibody [NBP1-83904] - Staining of human cervix shows moderate membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/FGF-BP-Antibody-Immunohistochemistry-Paraffin-NBP1-83904-img0012.jpg)
![Immunohistochemistry-Paraffin: FGF-BP Antibody [NBP1-83904] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FGF-BP-Antibody-Immunohistochemistry-Paraffin-NBP1-83904-img0013.jpg)
![Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Western-Blot-NBP2-22526-img0001.jpg "Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0006.jpg)
![Immunohistochemistry: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 showing staining in the membrane of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunohistochemistry-NBP2-22526-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0003.jpg)
![Immunoprecipitation: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunoprecipitation-NBP2-22526-img0002.jpg)