| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-153SS | |||
| Azide and BSA Free | NBP2-80870 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-48002-0.1mg | |||
| IHC-Prediluted | NBP2-48186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48318 | |||
| Azide and BSA Free | NBP3-11498 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87925-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87324-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71691SS | |||
![Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Western-Blot-NB300-153-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of Neurofibromin 1was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Immunohistochemistry-Paraffin-NB300-153-img0005.jpg)
![Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw 264.7 whole cell lysate/extract. G. 30 ug C2Cl2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-33713-img0008.jpg "Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw 264.7 whole cell lysate/extract. G. 30 ug C2Cl2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000")
![Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by Western blot analysis. A. 30 ug A431 whole cell lysate/extract whole cell lysate/extract. B. 30 ug H1299 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug Molt-4 whole cell lysate/extract. F. 30 ug Raji whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000 SET antibody detects SET protein by Western blot analysis.Observed SET protein is larger than the predicted M.W., possibly due to post-translational](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-33713-img0004.jpg)
![Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-33713-img0005.jpg)
![Immunoprecipitation: SET Antibody [NBP1-33713] - SET antibody immunoprecipitates SET protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of SET protein by 4 ug SET antibody. 10 % SDS-PAGE. The immunoprecipitated SET protein was detected by SET antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SET-Antibody-Immunoprecipitation-NBP1-33713-img0006.jpg)
![Western Blot: SET Antibody [NBP1-33713] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody (NBP1-33713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33713_rabbit-polyclonal-set-antibody-910202420115093.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SET Antibody [NBP1-33713] - Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-33713_rabbit-polyclonal-set-antibody-1610202419414526.jpg)
![SET antibody detects SET protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SET protein stained by SET antibody (NBP1-33713) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-33713_rabbit-polyclonal-set-antibody-immunocytochemistry-immunofluorescence-2611202518431924.jpg)
![Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate (50 ug) from HeLa, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SASH1-Antibody-Western-Blot-NBP1-26650-img0004.jpg "Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate (50 ug) from HeLa, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate from HeLa cells. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody NBP1-26651.](http://images.novusbio.com/fullsize/SASH1-Antibody-Western-Blot-NBP1-26650-img0003.jpg)
![Western Blot: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Western-Blot-NBP2-44975-img0006.jpg "Western Blot: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774). Image using the Azide and BSA Free format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (HSPB1/774) [NBP2-44975] - ICC/IF analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunofluorescence-NBP2-44975-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunohistochemistry-Paraffin-NBP2-44975-img0002.jpg)
![Flow Cytometry: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Flow-Cytometry-NBP2-44975-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)](http://images.novusbio.com/fullsize/HSP27-Antibody-HSPB1-774-Immunohistochemistry-Paraffin-NBP2-44975-img0001.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19460] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19460-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody [NBP2-19460] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: N Cadherin Antibody [NBP2-19460] - Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19460-img0002.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19460] - Paraffin-embedded hepatoma. N-Cadherin antibody [N2C1], Internal dilution: 1:500.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19460-img0003.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19460] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-91020242014643.jpg)
![N-Cadherin antibody [N2C1], Internal detects N-Cadherin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-immunohistochemistry-frozen-261120251842318.jpg)
![Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251828100.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251846100.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0006.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (SPM614) [NBP2-44611] - Human Tonsil stained with Nucleolin Monoclonal Antibody (SPM614).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunohistochemistry-Paraffin-NBP2-44611-img0001.jpg)
![Flow Cytometry: Nucleolin Antibody (SPM614) [NBP2-44611] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (SPM614).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Flow-Cytometry-NBP2-44611-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of HeLa cells stained with CF647R labeled Nucleolin Antibody (SPM614)(Green) Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0005.jpg)
![Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Western-Blot-NBP2-34621-img0002.jpg "Western Blot: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34621-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0004.jpg)
![Flow Cytometry: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34621-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) - Azide and BSA Free [NBP2-34621] - Formalin-fixed. paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34621-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87925] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87925-img0007.jpg "Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87925] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center.")
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0011.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human cervix, uterine shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0008.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0009.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0010.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/nbp1-87925_rabbit-polyclonal-flightless-i-antibody-65202516291312.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining in human placenta and skeletal muscle tissues . Corresponding GRN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87324-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining in human placenta and skeletal muscle tissues . Corresponding GRN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-87324] - Staining of human cell line A-431 shows localization to endoplasmic reticulum & lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87324-img0009.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87324-img0011.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87324-img0012.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human placenta shows moderate to strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87324-img0013.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in striated muscle fibers.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87324-img0014.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249403838.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249393545.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249432868.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dicer stained by Dicer antibody [HL1232] (NBP3-25439) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249362777.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-212024939176.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Dicer stained by Dicer antibody [HL1232] (NBP3-25439) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249371566.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249362730.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71691-img0008.jpg "Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-71691] - Analysis of Dicer in paraffin embedded human colorectal cancer tissue using anti-Dicer antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP1-71691-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - Dicer antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71691-img0005.jpg)
![Immunohistochemistry: Dicer Antibody [NBP1-71691] - Staining of Dicer in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-NBP1-71691-img0006.jpg)
![Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Western-Blot-NBP2-22527-img0006.jpg "Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunocytochemistry-Immunofluorescence-NBP2-22527-img0003.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human colon carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0005.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0004.jpg)
![Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunoprecipitation-NBP2-22527-img0002.jpg)