| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47781-0.1mg | |||
| IHC-Prediluted | NBP2-48351 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22118SS | |||
| Azide and BSA Free | NBP2-80587 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27177SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44732 | |||
| Azide and BSA Free | NBP2-34700-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86805-25ul | |||
![Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Western-Blot-NBP2-37563-img0003.jpg "Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (5H7) [NBP2-37563] - Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunocytochemistry-Immunofluorescence-NBP2-37563-img0004.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0013.jpg)
![Flow Cytometry: Prohibitin Antibody (5H7) [NBP2-37563] - Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Flow-Cytometry-NBP2-37563-img0005.jpg)
![Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mAb against human PHB (AA: 68-259) recombinant protein. (Expected MW is 46.7 kDa)](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Western-Blot-NBP2-37563-img0002.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0010.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0011.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0012.jpg)
![ELISA: Prohibitin Antibody (5H7) [NBP2-37563] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-ELISA-NBP2-37563-img0001.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Human Bladder Carcinoma stained with p21/CIP1/CDKN1A Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Immunohistochemistry-Paraffin-NBP2-44525-img0006.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Human Bladder Carcinoma stained with p21/CIP1/CDKN1A Antibody (DCS-60.2).")
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Simple Western lane view shows a specific band for p21/CIP1/CDKN1A in 0.2 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Simple-Western-NBP2-44525-img0002.jpg)
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Electropherogram image of the corresponding Simple Western lane. p21/CIP1/CDKN1A antibody was used at 10 ug/ml dilution of MCF-7 lysates(s) respectively.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Simple-Western-NBP2-44525-img0003.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/nbp2-44525_mouse-monoclonal-p21-cip1-cdkn1a-antibody-dcs-60-2-17720249362616.jpg)
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Lane view shows a specific band for p21/CIP1/CDKN1A using 2 mg/mL of MCF-7 cell lysate and 10 ug/mL antibody dilution. Electropherogram image of corresponding Simple Western lane view. Image reported by internal validation.](http://images.novusbio.com/fullsize/nbp2-44525_mouse-monoclonal-p21-cip1-cdkn1a-antibody-dcs-60-2-17720241044618.jpg)
![Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Western-Blot-NBP2-22118-img0002.jpg "Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Immunocytochemistry-Immunofluorescence-NBP2-22118-img0004.jpg)
![Flow Cytometry: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Flow-Cytometry-NBP2-22118-img0003.jpg)
![ELISA: Aurora A Antibody (1F8) [NBP2-22118] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-ELISA-NBP2-22118-img0001.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer. MDM2 stained by MDM2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0028.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer, using MDM2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091472.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242010445.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091496.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - MDM2/HDM2 antibody detects MDM2/HDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-910202420134015.jpg)
![MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 ug Jurkat cell whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody (NBP2-17247)7.5 % SDS-PAGEThe immunoprecipitated MDM2 protein was detected by MDM2 antibody (NBP2-17247) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-immunoprecipitation-261120251826130.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Western-Blot-NBP2-24695-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-24695-img0005.jpg)
![Western Blot: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/AIF-Antibody-Knockout-Validated-NBP2-27177-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: AIF Antibody [NBP2-27177] - Analysis of AIF in human colon using this antibody. Lane 1, normal colon. Lanes 2-7, colon carcinoma tissue lysatesfrom 6 different patients. mAIF, thought to represent mitochondrial AIF.](http://images.novusbio.com/fullsize/AIF-Antibody-Western-Blot-NBP2-27177-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-27177] - Staining of AIF in formalin-fixed, paraffin-embedded normal human colon using this antibody at 1:2000. Hematoxylin-eosin counterstain. In this example, AIF expression is predominant in the upper part of the crypt.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-27177-img0002.jpg)
![Simple Western: AIF Antibody [NBP2-27177] - Simple Western lane view shows a specific band for AIF in 0.2 mg/ml of Jurkat and MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/AIF-Antibody-Simple-Western-NBP2-27177-img0003.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0026.jpg "Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human ovarian carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0023.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug K562 whole cell lysate/extract B. 30 ug THP-1 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0019.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug BCL-1 whole cell lysate/extract. C. 30 ug Raw264.7 whole cell lysate/extract. 10 % SDS-PAGE. Dilution: 1:1000.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0020.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug HL-60 whole cell lysate/extract.10 % SDS-PAGE. Dilution: 1:1000](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0021.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human breast carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0022.jpg)
![Immunoprecipitation: BMI-1 Antibody [NBP1-33748] - Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of BMI-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of BMI-1 protein by 2.5 ug of BMI-1antibody 10% SDS-PAGE The immunoprecipitated BMI-1protein was detected by BMI-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NBP1-33748-img0018.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-9102024209145.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog lung.BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420115062.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202419593519.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420131285.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242021927.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420134048.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242063358.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-33748] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-1610202419414590.jpg)
![BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: BMI1 protein stained by BMI1 antibody (NBP1-33748) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-immunocytochemistry-immunofluorescence-261120251844101.jpg)
![Western Blot: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Analysis using the Azide and BSA Free version of NBP2-32887. Detection of ODC1 in human placental lysate](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Western-Blot-NBP2-32887-img0004.jpg "Western Blot: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Analysis using the Azide and BSA Free version of NBP2-32887. Detection of ODC1 in human placental lysate")
![Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - IF staining of LNCap cells using AF488 labeled ODC1 Monoclonal Antibody (ODC1/485) (GREEN) DAPI was used to stain the cell nucleus (blue). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Immunocytochemistry-Immunofluorescence-NBP2-32887-img0014.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Ornithine Decarboxylase Antibody (ODC1/485).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Immunohistochemistry-Paraffin-NBP2-32887-img0012.jpg)
![Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Flow Cytometric Analysis of human ODC-1 in PC3 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ornithine Decarboxylase antibody (ODC1/485).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Flow-Cytometry-NBP2-32887-img0015.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded human prostate carcinoma with ODC-1 Monoclonal Antibody (ODC1/485)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Immunohistochemistry-Paraffin-NBP2-32887-img0005.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded human Testicular carcinoma with ODC-1 Monoclonal Antibody (ODC1/485)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Immunohistochemistry-Paraffin-NBP2-32887-img0006.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded Rat pancreas stained with ODC1 Monoclonal Antibody (ODC1/485)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Immunohistochemistry-Paraffin-NBP2-32887-img0009.jpg)
![Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody (M01), clone 3G2.Lane 1: DUSP6 transfected lysate(42 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Western-Blot-H00001848-M01-img0021.jpg "Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody (M01), clone 3G2.Lane 1: DUSP6 transfected lysate(42 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of monoclonal antibody to DUSP6 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Immunocytochemistry-Immunofluorescence-H00001848-M01-img0016.jpg)
![Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Immunohistochemistry-Paraffin-H00001848-M01-img0018.jpg)
![Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - DUSP6 monoclonal antibody (M01), clone 3G2 Western Blot analysis of DUSP6 expression in Jurkat ( Cat # L017V1 ).](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Western-Blot-H00001848-M01-img0012.jpg)
![Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - DUSP6 monoclonal antibody (M01), clone 3G2. Western Blot analysis of DUSP6 expression in human placenta.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Western-Blot-H00001848-M01-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi ( Cat # H00001848-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody (M01), clone 3G2 (Cat # H00001848-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Western-Blot-H00001848-M01-img0019.jpg)
![ELISA: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Detection limit for recombinant GST tagged DUSP6 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-ELISA-H00001848-M01-img0022.jpg)
![Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2. Analysis of PHB expression in NIH/3T3.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Western-Blot-H00005245-M01-img0015.jpg "Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2. Analysis of PHB expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Immunocytochemistry-Immunofluorescence-H00005245-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human tonsil tissue.Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Immunohistochemistry-Paraffin-H00005245-M01-img0011.jpg)
![Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2 Analysis of PHB expression in Hela.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Western-Blot-H00005245-M01-img0013.jpg)
![Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2. Analysis of PHB expression in PC-12.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Western-Blot-H00005245-M01-img0014.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human colon adenocarcinoma. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Immunohistochemistry-Paraffin-H00005245-M01-img0009.jpg)
![Sandwich ELISA: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Detection limit for recombinant GST tagged PHB is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-3F4-2B2-Sandwich-ELISA-H00005245-M01-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex, gallbladder, kidney and lymph node using Anti-EML4 antibody NBP1-86805 (A) shows similar protein distribution across tissues to independent antibody NBP2-38319 (B).](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0007.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human lymph node.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0010.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0008.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human kidney.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0009.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human gallbladder.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0011.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-86805_rabbit-polyclonal-eml4-antibody-304202513101431.jpg)