| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48309SS | |||
| Azide and BSA Free | NBP2-80868 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54153SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-2860-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72891 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56462SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38856-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44495 | |||
| BSA Free | NBP2-61632-25ul | |||
![Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunostaining of original tumor, low passage, and high passage KCI-MENG1 cells. The original patient-derived tumor (top row) showed moderate and patchy immunoreactivity for epithelial membrane antigen (EMA); strong and diffuse immunostaining for progesterone receptor (PR); and a Ki-67 proliferative index of 2-3%. There was also strong immunostaining for N-cadherin and vimentin. KCI-MENG1-LP cells (middle row) and KCI-MENG1-HP cells (bottom row) maintained expression of EMA, N-cadherin, and vimentin but had significantly reduced PR expression compared to the original tumor. Whereas Ki-67 labeling was found in only a small number of cells in the original tumor and low passage cells, it was positive in virtually all P84 cells. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-NBP1-48309-img0025.jpg "Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunostaining of original tumor, low passage, and high passage KCI-MENG1 cells. The original patient-derived tumor (top row) showed moderate and patchy immunoreactivity for epithelial membrane antigen (EMA); strong and diffuse immunostaining for progesterone receptor (PR); and a Ki-67 proliferative index of 2-3%. There was also strong immunostaining for N-cadherin and vimentin. KCI-MENG1-LP cells (middle row) and KCI-MENG1-HP cells (bottom row) maintained expression of EMA, N-cadherin, and vimentin but had significantly reduced PR expression compared to the original tumor. Whereas Ki-67 labeling was found in only a small number of cells in the original tumor and low passage cells, it was positive in virtually all P84 cells. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.")
![Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Western blots showing a reduction in epithelial mesenchymal transition (EMT) markers upon PDK1 knockdown in Colo205 and HT29 cells using E cadherin (NBP2-19051), N cadherin (NBP1-48309) and B-actin antibody (NB600-501). The corresponding secondary antibodies used were either goat anti-rabbit IgG-HRP (NB7160) or goat anti-mouse IgG-HRP (NB7539). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Western-Blot-NBP1-48309-img0029.jpg)
![Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N-Cadherin expression in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Western-Blot-NBP1-48309-img0005.jpg)
![Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Human meningioma mouse xenograft model KCI-MENG1-LPSX generated with the spontaneously immortal cell line KCI-MENG1-LP. Tumors from immunocompromised SCID mice were dissected and the derivative cell line KCI-MENG1-LPSX CL was generated. The EMA, PR, and N-cadherin IHC of the mouse tumor highly resembled the original patient-derived tumor. The vimentin- and Ki-67-stained cells in the mouse tumor tissue were markedly more abundant and more intensely stained than in the original tumor. KCI-MENG1-LPSX CL cells displayed the same patterns of immunostaining as the high passage parent cell line KCI-MENG1-HP, including the loss of PR staining. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-NBP1-48309-img0026.jpg)
![Flow (Intracellular): N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa with NBP1-48309 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Flow-Intracellular-NBP1-48309-img0010.jpg)
![Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Simple-Western-NBP1-48309-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Mitochondrial fission regulates migration and invasion in BRAF^V600E CRC cells through glucose metabolic reprograming. Western blot showing a reduction in EMT markers upon vemurafenib treatment in BRAF^V600E cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Western-Blot-NBP1-48309-img0032.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - Adult mouse neural stem cells stained for N-Cadherin. Antibody at 1:100. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-Immunofluorescence-NBP1-48309-img0027.jpg)
![Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunohistochemical characterization of tissue cultures. Nuclei labeled with DAPI (blue). Whole-mount immunostaining of a cardiomyocyte-only spheroid 1 month in culture, N-cadherin (red) and EH-myomesin (green). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32118040/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunohistochemistry-NBP1-48309-img0031.jpg)
![Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on U-251 MG cells with N-Cadherin Antibody (13A9) NBP1-48309AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Flow-Cytometry-NBP1-48309-img0030.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HEK 293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti- at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-Immunofluorescence-NBP1-48309-img0021.jpg)
![Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunohistochemistry-NBP1-48309-img0007.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of N-Cadherin antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunohistochemistry-Paraffin-NBP1-48309-img0028.jpg)
![Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Flow-Cytometry-NBP1-48309-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MyoD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0005.jpg "Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: MyoD1 Antibody [NBP1-54153] - Detection of MYOD1 in RH30 cells.](http://images.novusbio.com/fullsize/MyoD1-Antibody-Western-Blot-NBP1-54153-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - Staing of MYOD1 in RD cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MyoD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0003.jpg)
![Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Western-Blot-NB120-2860-img0028.jpg "Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.")
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0008.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat testis tissue.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunohistochemistry-Paraffin-NB120-2860-img0013.jpg)
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in MCF-7 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.A2058 melanoma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. C6 glioma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. HeLa Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0017.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat cerebellum tissue.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunohistochemistry-Paraffin-NB120-2860-img0012.jpg)
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in C6 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0018.jpg)
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in PC12 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0019.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19457] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19457-img0019.jpg "Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19457] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Frozen: N-Cadherin Antibody [NBP2-19457] - Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Frozen-NBP2-19457-img0007.jpg)
![N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody (NBP2-19457) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19457_rabbit-polyclonal-n-cadherin-antibody-immunohistochemistry-frozen-2611202518224321.jpg)
![Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] Analysis of extracts (35ug) from 9 different cell lines by using anti-N Cadherin monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Western-Blot-NBP2-01498-img0008.jpg "Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] Analysis of extracts (35ug) from 9 different cell lines by using anti-N Cadherin monoclonal antibody.")
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Human liver tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0007.jpg)
![Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-N Cadherin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Flow-Cytometry-NBP2-01498-img0002.jpg)
![Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY N Cadherin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-N Cadherin.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Western-Blot-NBP2-01498-img0003.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0004.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0005.jpg)
![Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-N Cadherin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-01498-img0006.jpg)
![Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - Analysis of Hela cells, using anti-N Cadherin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-2G7-Flow-Cytometry-NBP2-01498-img0001.jpg)
![Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Western-Blot-NB100-56462-img0004.jpg "Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: Smad2 Antibody [NB100-56462] - Mouse testis tissue probed with this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Immunohistochemistry-Paraffin-NB100-56462-img0003.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19459] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19459-img0021.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody [NBP2-19459] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19459-img0019.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19459] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19459-img0014.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19459] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19459-img0023.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Human hepatoma. N-Cadherin antibody [N1N3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19459-img0008.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Rat liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19459-img0018.jpg)
![Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (NBP2-19459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19459_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251842291.jpg)
![N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: N-Cadherin stained by N-Cadherin antibody [N1N3] (NBP2-19459) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-19459_rabbit-polyclonal-n-cadherin-antibody-immunocytochemistry-immunofluorescence-261120251825260.jpg)
![N-Cadherin antibody [N1N3] detects N-Cadherin protein by western blot analysis.A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract5% SDS-PAGEN-Cadherin antibody [N1N3] (NBP2-19459) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19459_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251829017.jpg)
![Western Blot: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of Calmodulin on rat brain lysates using anti-Calmodulin antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Western-Blot-NBP2-67413-img0001.jpg "Western Blot: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of Calmodulin on rat brain lysates using anti-Calmodulin antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunocytochemistry-Immunofluorescence-NBP2-67413-img0005.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunohistochemistry-Paraffin-NBP2-67413-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunocytochemistry-Immunofluorescence-NBP2-67413-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunocytochemistry-Immunofluorescence-NBP2-67413-img0004.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of paraffin-embedded mouse brain tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunohistochemistry-Paraffin-NBP2-67413-img0006.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining in human heart muscle and skin tissues.. Corresponding CDH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining in human heart muscle and skin tissues.. Corresponding CDH2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody [NBP2-38856] - Disruption of N-cadherin shortens burst duration without affecting other calcium spike parameters. Immunohistochemistry showing N-cadherin (red, upper left), K-cadherin (red, lower left) and F-actin (green, middle) are abundant in the zG layer (right, merged images). Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15408-4), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38856-img0010.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0009.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0006.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human skin shows no positivity in keratinocytes.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0007.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38856-img0008.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [NBP3-15654] - Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (NBP3-15654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-6F9G8-Knockout-Validated-NBP3-15654-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody (6F9G8) [NBP3-15654] - Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (NBP3-15654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311049.jpg)
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Confocal imaging of paraffin-embedded rat heart using [KO Validated] N-Cadherin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517352755.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Western blot analysis of lysates from C2C12 cells using [KO Validated] N-Cadherin Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517304949.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-522025173458.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517344234.jpg)
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Confocal imaging of paraffin-embedded Mouse heart using [KO Validated] N-Cadherin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311076.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517361268.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Western blot analysis of lysates from C6 cells using [KO Validated] N-Cadherin Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311081.jpg)
![Western Blot: N-Cadherin Antibody (CL3716) [NBP2-61632] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Western-Blot-NBP2-61632-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody (CL3716) [NBP2-61632] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining in human heart muscle and skin tissues. Corresponding CDH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of U-251 cells using the Anti-CDH2 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunocytochemistry-Immunofluorescence-NBP2-61632-img0010.jpg)
![Western Blot: N-Cadherin Antibody (CL3716) [NBP2-61632] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Western-Blot-NBP2-61632-img0009.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human heart muscle shows strong positivity in the intercalated discs of cardiomyocytes.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0002.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0003.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0004.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human liver cancer (hepatocellular carcinoma) shows moderate to strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0005.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human skin shows no positivity in epidermal cells as expected.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0006.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19460] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19460-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody [NBP2-19460] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: N Cadherin Antibody [NBP2-19460] - Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19460-img0002.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19460] - Paraffin-embedded hepatoma. N-Cadherin antibody [N2C1], Internal dilution: 1:500.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19460-img0003.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19460] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-91020242014643.jpg)
![N-Cadherin antibody [N2C1], Internal detects N-Cadherin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-immunohistochemistry-frozen-261120251842318.jpg)
![Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251828100.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19460_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251846100.jpg)