| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74571SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80611-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71565 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61780-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80762-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83036-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49172-25ul | |||
![Western Blot: Septin-12 Antibody [H00124404-B01P] - Analysis of SEPT12 expression in transfected 293T cell line by SEPT12 polyclonal antibody. Lane 1: SEPT12 transfected lysate(39.38 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Western-Blot-H00124404-B01P-img0006.jpg "Western Blot: Septin-12 Antibody [H00124404-B01P] - Analysis of SEPT12 expression in transfected 293T cell line by SEPT12 polyclonal antibody. Lane 1: SEPT12 transfected lysate(39.38 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - An IFA revealed multiple localizations of SEPT12 and LAMINB1 signals. A total of 32 male germ cells were were evaluated as (a.) Round spermatids (n = 9; labeled with anti-SEPT12 and anti-LAMINB1 antibodies as the presented figure,9/9); (b.) elongating and (c.) elongated spermatids (n = 11;11/11); (d.) ejaculated spermatozoa (n = 10;10/10). (a.-d.) Anti-SEPT12 antibody (left panel; green), anti-LAMINB1 antibody (middle panel; red), and a combination of the left and middle panels (right panel). The arrow indicates SEPT12 or LAMINB1 signals. Scale bar: 5 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120722) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunocytochemistry-Immunofluorescence-H00124404-B01P-img0007.jpg)
![Immunohistochemistry-Paraffin: Septin-12 Antibody [H00124404-B01P] - Analysis of purified antibody to SEPT12 on formalin-fixed paraffin-embedded human colon. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunohistochemistry-Paraffin-H00124404-B01P-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - Analysis of purified antibody to SEPT12 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunocytochemistry-Immunofluorescence-H00124404-B01P-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - SEPT12 interacts & colocalizes with LAMINB1 male germ cells.(A) Co-IP of FLAG-SPAG4 & LAIMINB1 with SEPT12-GFP. The cell lysates of the NT2D1 cells, transfected with pEGFP-SEPT12 & a pFLAG-SPAG4 vector, are subjected to IP with an anti-GFP antibody (right panel, Row 3) or a nonspecific control IgG (right panel, Row 2), followed by IB with an anti-GFP, anti-FLAG, or anti-LAMINB1 antibody. An input protein (5%) was used as a control during the IB of the transfected cell lysates (right panel, Row 1). (B) An IFA revealed multiple localizations of SEPT12 & LAMINB1 signals. A total of 32 male germ cells were were evaluated as (A) Round spermatids (n = 9; labeled with anti-SEPT12 & anti-LAMINB1antibodies as the presented figure,9/9); (B) elongating & (C) elongated spermatids (n = 11;11/11); (D) ejaculated spermatozoa (n = 10;10/10). (A–D) Anti-SEPT12 antibody (left panel; green), anti-LAMINB1 antibody (middle panel; red), & a combination of the left & middle panels (right panel). The arrow indicates SEPT12 or LAMINB1 signals. Scale bar: 5 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120722), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00124404-b01p_mouse-polyclonal-septin-12-antibody-310202415483631.jpg)
![Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NB110-74571-img0004.jpg "Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.")
![Immunocytochemistry/Immunofluorescence: Striatin Antibody [NB110-74571] - Immunofluorescent staining of NIH3T3 cells ± blocking peptide using NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74571-img0003.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Analysis in human cerebral cortex and pancreas tissues. Corresponding YWHAB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Analysis in human cerebral cortex and pancreas tissues. Corresponding YWHAB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: 14-3-3 beta/alpha Antibody [NBP1-80611] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-80611-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80611-img0003.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0010.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-80611] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-80611-img0004.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0007.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human skin shows moderate to strong cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0009.jpg)
![Western Blot: DNAJA2 Antibody (3A10) [NBP2-02996] Analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJA2 monoclonal antibody.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Western-Blot-NBP2-02996-img0013.jpg "Western Blot: DNAJA2 Antibody (3A10) [NBP2-02996] Analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJA2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJA2.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Immunocytochemistry-Immunofluorescence-NBP2-02996-img0012.jpg)
![Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Human thyroid tissue using anti-DNAJA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Immunohistochemistry-Paraffin-NBP2-02996-img0011.jpg)
![Flow Cytometry: DNAJA2 Antibody (3A10) [NBP2-02996] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DNAJA2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Flow-Cytometry-NBP2-02996-img0001.jpg)
![Western Blot: DNAJA2 Antibody (3A10) [NBP2-02996] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJA2.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Western-Blot-NBP2-02996-img0002.jpg)
![Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DNAJA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Immunohistochemistry-Paraffin-NBP2-02996-img0003.jpg)
![Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DNAJA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Immunohistochemistry-Paraffin-NBP2-02996-img0004.jpg)
![Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DNAJA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Immunohistochemistry-Paraffin-NBP2-02996-img0005.jpg)
![Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DNAJA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Immunohistochemistry-Paraffin-NBP2-02996-img0006.jpg)
![Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Human breast tissue using anti-DNAJA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Immunohistochemistry-Paraffin-NBP2-02996-img0007.jpg)
![Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Human Kidney tissue using anti-DNAJA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Immunohistochemistry-Paraffin-NBP2-02996-img0008.jpg)
![Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Human lymph node tissue using anti-DNAJA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Immunohistochemistry-Paraffin-NBP2-02996-img0009.jpg)
![Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Human lymphoma tissue using anti-DNAJA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-3A10-Immunohistochemistry-Paraffin-NBP2-02996-img0010.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0009.jpg "Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of HepG2 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0005.jpg)
![Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded endometrial cancer tissues using YWHAB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunohistochemistry-NBP2-61780-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against human YWHAB (AA: 1-246) recombinant protein. (Expected MW is 54 kDa)](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0008.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mouse mAb against Hela (1), NIH/3T3 (2), C6 (3), A431 (4), K562 (5), PC-12 (6), and U937 (7) cell lysate.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of GC-7901 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0004.jpg)
![Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded rectum cancer tissues using YWHAB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunohistochemistry-NBP2-61780-img0006.jpg)
![ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-ELISA-NBP2-61780-img0001.jpg)
![ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-ELISA-NBP2-61780-img0002.jpg)
![Western Blot: Striatin Antibody [NBP1-80762] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NBP1-80762-img0011.jpg "Western Blot: Striatin Antibody [NBP1-80762] - Analysis in human cell line RH-30.")
![Immunocytochemistry/Immunofluorescence: Striatin Antibody [NBP1-80762] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/Striatin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80762-img0010.jpg)
![Immunohistochemistry-Paraffin: Striatin Antibody [NBP1-80762] - Staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Striatin-Antibody-Immunohistochemistry-Paraffin-NBP1-80762-img0009.jpg)
![Western Blot: Striatin Antibody [NBP1-80762] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NBP1-80762-img0005.jpg)
![Western Blot: Striatin Antibody [NBP1-80762] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NBP1-80762-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33454-img0003.jpg "Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Rat cervix. 14-3-3 beta antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-33454-img0005.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33454_rabbit-polyclonal-14-3-3-beta-alpha-antibody-western-blot-2611202518274621.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000 and competitor's antibody (sc-628) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33454_rabbit-polyclonal-14-3-3-beta-alpha-antibody-western-blot-2611202518431912.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-278202415295566.jpg "Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-298202414333627.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunocytochemistry analysis of HeLa cells labeling Casein Kinase 2 alpha with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-2920241192348.jpg)
![Flow Cytometry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Flow cytometric analysis of HeLa cells labeling Casein Kinase 2 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32228, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-29202416453220.jpg)
![Western Blot: DNAJA2 Antibody (2A11-F2) [H00010294-M01] - DNAJA2 monoclonal antibody (M01), clone 2A11-F2 Analysis of DNAJA2 expression in A-431.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-2A11-F2-Western-Blot-H00010294-M01-img0006.jpg "Western Blot: DNAJA2 Antibody (2A11-F2) [H00010294-M01] - DNAJA2 monoclonal antibody (M01), clone 2A11-F2 Analysis of DNAJA2 expression in A-431.")
![Immunocytochemistry/Immunofluorescence: DNAJA2 Antibody (2A11-F2) [H00010294-M01] - Analysis of monoclonal antibody to DNAJA2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-2A11-F2-Immunocytochemistry-Immunofluorescence-H00010294-M01-img0005.jpg)
![ELISA: DNAJA2 Antibody (2A11-F2) [H00010294-M01] - Detection limit for recombinant GST tagged DNAJA2 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DNAJA2-Antibody-2A11-F2-ELISA-H00010294-M01-img0003.jpg)
![Western Blot: MORC3 Antibody [NBP1-83036] - Total and purified protein fractions from mock (m) & wt HSV-1 (wt; MOI 10 12 h p.i.) infected HA-HisSUMO2 cells analyzed by WB (uninfected HA-His only total protein & purified fractions as controls; mock infected = m; infected samples = wt). Probable sumoylated species of PML, NACC1, ZBTB10, ZBTB38, ZBTB4 and MORC3 detected in uninfected purified samples (indicated by dashes on the right of panels), & reduced in abundance during HSV-1 infection. ZBTB7A showed increased SUMO2-modification in infected purified fractions. RanGAP1 used as an unchanged control for SUMO2-modification following HSV-1 infection. The predicted unmodified molecular weight of each protein is indicated in each panel; major unmodified form is marked by an asterisk - this refers to the major isoforms PML.I and PML.II. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1005059), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MORC3-Antibody-Western-Blot-NBP1-83036-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: MORC3 Antibody [NBP1-83036] - Total and purified protein fractions from mock (m) & wt HSV-1 (wt; MOI 10 12 h p.i.) infected HA-HisSUMO2 cells analyzed by WB (uninfected HA-His only total protein & purified fractions as controls; mock infected = m; infected samples = wt). Probable sumoylated species of PML, NACC1, ZBTB10, ZBTB38, ZBTB4 and MORC3 detected in uninfected purified samples (indicated by dashes on the right of panels), & reduced in abundance during HSV-1 infection. ZBTB7A showed increased SUMO2-modification in infected purified fractions. RanGAP1 used as an unchanged control for SUMO2-modification following HSV-1 infection. The predicted unmodified molecular weight of each protein is indicated in each panel; major unmodified form is marked by an asterisk - this refers to the major isoforms PML.I and PML.II. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1005059), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MORC3 Antibody [NBP1-83036] - Staining of human esophagus shows nuclear and cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/MORC3-Antibody-Immunohistochemistry-Paraffin-NBP1-83036-img0006.jpg)
![Western Blot: MORC3 Antibody [NBP1-83036] - Analysis in human cell line SiHa.](http://images.novusbio.com/fullsize/MORC3-Antibody-Western-Blot-NBP1-83036-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: MORC3 Antibody [NBP1-83036] - Influence of a SUMO interaction motif within ICP0 on the stability of selected proteins during HSV-1 infection. HepaRG cells were infected with wt or mSLS4 mutant HSV-1 and samples taken at 3, 6 and 10 h after infection were analyzed for the abundance of NACC1, MORC3 and ZBTB38 as indicated. The MOI were adjusted to ensure similar levels of viral gene expression (10 for wt, 20 for mSLS4), as shown in the ICP0, ICP4 and UL42 panels. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1005059), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MORC3-Antibody-Western-Blot-NBP1-83036-img0009.jpg)
![Western Blot: p190RhoGAP Antibody [NBP2-49172] - Analysis in human cell line WM-115.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Western-Blot-NBP2-49172-img0006.jpg "Western Blot: p190RhoGAP Antibody [NBP2-49172] - Analysis in human cell line WM-115.")
![Immunohistochemistry-Paraffin: p190RhoGAP Antibody [NBP2-49172] - Staining of human fallopian tube shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Immunohistochemistry-Paraffin-NBP2-49172-img0005.jpg)
![Immunohistochemistry-Paraffin: p190RhoGAP Antibody [NBP2-49172] - Staining of human breast shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Immunohistochemistry-NBP2-49172-img0001.jpg)
![Immunohistochemistry-Paraffin: p190RhoGAP Antibody [NBP2-49172] - Staining of human testis shows moderate membranous and cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Immunohistochemistry-Paraffin-NBP2-49172-img0002.jpg)
![Immunohistochemistry-Paraffin: p190RhoGAP Antibody [NBP2-49172] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Immunohistochemistry-Paraffin-NBP2-49172-img0003.jpg)
![Immunohistochemistry-Paraffin: p190RhoGAP Antibody [NBP2-49172] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Immunohistochemistry-Paraffin-NBP2-49172-img0004.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - Human Fetal Brain, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0004.jpg "Western Blot: Septin-11 Antibody [NBP1-58144] - Human Fetal Brain, Antibody Dilution: 1.0 ug/ml.")
![Immunohistochemistry-Paraffin: Septin-11 Antibody [NBP1-58144] - Human Spleen Tissue, 5 ug/ml.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Immunohistochemistry-Paraffin-NBP1-58144-img0003.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - 1. Human NT-2 cells (60ug) 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody : IRDye 800CW goat anti-rabbit Secondary Antibody Dilution: 1: 20,000](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0001.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - NT-2 cell line, mouse brain, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0002.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0005.jpg)