| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73271 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37822-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71351 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70314 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72106 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71650 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73481 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71240 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73857 | |||
![Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA.](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Western-Blot-NBP2-46342-img0022.jpg "Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA.")
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0021.jpg)
![Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of extracts (35ug) from 9 different cell lines by using PCNA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:2000](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Western-Blot-NBP2-46342-img0023.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0001.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0002.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0003.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0004.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0005.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0006.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0007.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0008.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0009.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0010.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0011.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0012.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0013.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0014.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0015.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0016.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0017.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0018.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0019.jpg)
![Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PCNA-Antibody-3D6-Immunohistochemistry-NBP2-46342-img0020.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the HRP conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0011.jpg "Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the HRP conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico.")
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0009.jpg)
![Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Flow-Cytometry-NBP2-37822-img0013.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis of 1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2)](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0005.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 488 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 550 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0008.jpg)
![Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on HepG2 cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Flow-Cytometry-NBP2-37822-img0012.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Western-Blot-NBP1-47757-img0019.jpg "Western Blot: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.")
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Carcinoma of bladder tissue.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0020.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0011.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0012.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0013.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0014.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded endometrium tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0015.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Kidney tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0016.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0017.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded prostate tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0018.jpg)
![Western Blot: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Western-Blot-NBP1-47680-img0002.jpg "Western Blot: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (OTI6E5) [NBP1-47680] - BubR1 Antibody (6E5) [NBP1-47680] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunocytochemistry-Immunofluorescence-NBP1-47680-img0012.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded prostate tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0009.jpg)
![Flow Cytometry: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells transfected with either pCMV6-ENTRY BubR1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-BubR1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Flow-Cytometry-NBP1-47680-img0011.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0003.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0004.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0005.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0006.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded endometrium tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0007.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded pancreas tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0008.jpg)
![Western Blot: CCNB1IP1 Antibody (4H3) [NBP2-00600] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNB1IP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNB1IP1.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Western-Blot-NBP2-00600-img0003.jpg "Western Blot: CCNB1IP1 Antibody (4H3) [NBP2-00600] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNB1IP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNB1IP1.")
![Immunohistochemistry-Paraffin: CCNB1IP1 Antibody (4H3) [NBP2-00600] Staining of paraffin-embedded Human prostate tissue using anti-CCNB1IP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Immunohistochemistry-Paraffin-NBP2-00600-img0008.jpg)
![Flow Cytometry: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Analysis of Jurkat cells, using anti-CCNB1IP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Flow-Cytometry-NBP2-00600-img0002.jpg)
![Immunohistochemistry-Paraffin: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CCNB1IP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Immunohistochemistry-Paraffin-NBP2-00600-img0004.jpg)
![Immunohistochemistry-Paraffin: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CCNB1IP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Immunohistochemistry-Paraffin-NBP2-00600-img0005.jpg)
![Immunohistochemistry-Paraffin: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Staining of paraffin-embedded Human lymph node tissue using anti-CCNB1IP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Immunohistochemistry-Paraffin-NBP2-00600-img0006.jpg)
![Immunohistochemistry-Paraffin: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Staining of paraffin-embedded Human pancreas tissue using anti-CCNB1IP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Immunohistochemistry-Paraffin-NBP2-00600-img0007.jpg)
![Flow Cytometry: CCNB1IP1 Antibody (4H3) [NBP2-00600] - Analysis of Hela cells, using anti-CCNB1IP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CCNB1IP1-Antibody-4H3-Flow-Cytometry-NBP2-00600-img0001.jpg)
![Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Western-Blot-NB100-74502-img0004.jpg "Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0010.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (13E8) [NB100-74502] - Both normal and cancer biopsies of deparaffinized human Colon tissue.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunohistochemistry-Paraffin-NB100-74502-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0009.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0008.jpg "Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.")
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HepG2 cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0003.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0007.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: Human; HeLa: Human; HT29: Human; A549: Human; COS7: monkey; Jurkat: human; MDCK: Canine; PC12: Rat; MCF7: human)](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0004.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0005.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0006.jpg)
![Western Blot: EB2 Antibody (1F3) [NBP2-00714] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EB2.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Western-Blot-NBP2-00714-img0004.jpg "Western Blot: EB2 Antibody (1F3) [NBP2-00714] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EB2.")
![Immunocytochemistry/Immunofluorescence: EB2 Antibody (1F3) [NBP2-00714] Staining of COS7 cells transiently transfected by pCMV6-ENTRY EB2.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP2-00714-img0009.jpg)
![Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Human pancreas tissue using anti-EB2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Immunohistochemistry-Paraffin-NBP2-00714-img0008.jpg)
![Flow Cytometry: EB2 Antibody (1F3) [NBP2-00714] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EB2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Flow-Cytometry-NBP2-00714-img0003.jpg)
![Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-EB2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Immunohistochemistry-Paraffin-NBP2-00714-img0005.jpg)
![Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EB2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Immunohistochemistry-Paraffin-NBP2-00714-img0006.jpg)
![Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EB2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Immunohistochemistry-Paraffin-NBP2-00714-img0007.jpg)
![Flow Cytometry: EB2 Antibody (1F3) [NBP2-00714] - Analysis of Hela cells, using anti-EB2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Flow-Cytometry-NBP2-00714-img0001.jpg)
![Flow Cytometry: EB2 Antibody (1F3) [NBP2-00714] - Analysis of Jurkat cells, using anti-EB2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Flow-Cytometry-NBP2-00714-img0002.jpg)
![Western Blot: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMS2.](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Western-Blot-NBP2-46459-img0008.jpg "Western Blot: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMS2.")
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0007.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0002.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0003.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0004.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0005.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunohistochemistry-NBP2-46459-img0006.jpg)
![Immunofluorescence: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/PMS2-Antibody-OTI2G5-Immunofluorescence-NBP2-46459-img0001.jpg)
![Western Blot: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK4 .](http://images.novusbio.com/fullsize/ERK4-MAPK4-Antibody-OTI2D7-Western-Blot-NBP2-45743-img0008.jpg "Western Blot: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK4 .")
![Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ERK4-MAPK4-Antibody-OTI2D7-Immunohistochemistry-NBP2-45743-img0007.jpg)
![Western Blot: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of extracts (10ug) from 8 Human tissue (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).](http://images.novusbio.com/fullsize/ERK4-MAPK4-Antibody-OTI2D7-Western-Blot-NBP2-45743-img0009.jpg)
![Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ERK4-MAPK4-Antibody-OTI2D7-Immunohistochemistry-NBP2-45743-img0002.jpg)
![Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ERK4-MAPK4-Antibody-OTI2D7-Immunohistochemistry-NBP2-45743-img0003.jpg)
![Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ERK4-MAPK4-Antibody-OTI2D7-Immunohistochemistry-NBP2-45743-img0004.jpg)
![Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ERK4-MAPK4-Antibody-OTI2D7-Immunohistochemistry-NBP2-45743-img0005.jpg)
![Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ERK4-MAPK4-Antibody-OTI2D7-Immunohistochemistry-NBP2-45743-img0006.jpg)
![Immunofluorescence: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK4/MAPK4.](http://images.novusbio.com/fullsize/ERK4-MAPK4-Antibody-OTI2D7-Immunofluorescence-NBP2-45743-img0001.jpg)
![Western Blot: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Western-Blot-NBP2-45570-img0013.jpg "Western Blot: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.")
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0012.jpg)
![Flow Cytometry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by RFC2 antibody.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Flow-Cytometry-NBP2-45570-img0001.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0002.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0003.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0004.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0005.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0006.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human Ovary tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0007.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0008.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0009.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0010.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/RFC2-Antibody-2B2-Immunohistochemistry-NBP2-45570-img0011.jpg)
![Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Western-Blot-NBP2-22527-img0006.jpg "Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunocytochemistry-Immunofluorescence-NBP2-22527-img0003.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human colon carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0005.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0004.jpg)
![Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunoprecipitation-NBP2-22527-img0002.jpg)