| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56341SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-330SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47769-0.1mg | |||
| IHC-Prediluted | NBP2-48399 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-153SS | |||
| Azide and BSA Free | NBP2-80870 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40823SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48318 | |||
| Azide and BSA Free | NBP3-11498 | |||
![Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-24533-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Analysis of HSP27 in human skeletal muscle lysate using 1) preimmune sera at 1:5000 and 2) peptide affinity purified HSP27 antibody at 2.0 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP2-24533-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP2-24533] - FFPE human small intestine probed with HSP27 antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-Paraffin-NBP2-24533-img0002.jpg)
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0012.jpg "Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Immunofluorescence Microscopy of HDAC1 antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum.Primary antibody: HDAC1 antibody at 4 ug/mL for 1 h at RT.Secondary antibody: rabbit secondary antibody at 0.2 ug/mL for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0010.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 10 ug/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as brown color indicates presence of protein, blue color shows cell nuclei.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0014.jpg)
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: 293 whole cell lysate. Load: 35 ug per lane.Primary antibody: HDAC1 antibody at 1:3,500 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Rabbit IgG 611-151-122 (shown in red) for to detect HDAC-1 by immunofluorescence. Anti Keratin monoclonal antibody was used with Dylight 488 goat anti mouse (shown in green) to detect Keratin](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0004.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 1:500 for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 precipitated purple with blue counterstain.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0013.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NB100-56341] - Analysis of HDAC-4 in Jurkat cell lysate with this antibody.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NB100-56341-img0003.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NB100-56341] - Analysis of HDAC-4 in Jurkat cell lysate with this antibody.")
![Immunocytochemistry/Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330] - Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using NB 100-330, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.](http://images.novusbio.com/fullsize/Separase-Antibody-XJ11-4D7-Immunocytochemistry-Immunofluorescence-NB100-330-img0003.jpg "Immunocytochemistry/Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330] - Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using NB 100-330, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.")
![Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (KIP1/769) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Immunocytochemistry-Immunofluorescence-NBP2-44495-img0007.jpg "Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (KIP1/769) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.")
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Rat Colon stained with p27 Monoclonal Antibody (KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Immunohistochemistry-Paraffin-NBP2-44495-img0005.jpg)
![Flow Cytometry: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Human p27 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (KIP1/769).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Flow-Cytometry-NBP2-44495-img0001.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Human Cervical cancer stained with p27 Monoclonal Antibody (KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Immunohistochemistry-Paraffin-NBP2-44495-img0002.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Human Colon Carcinoma stained with p27 Monoclonal Antibody (KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Immunohistochemistry-Paraffin-NBP2-44495-img0003.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Human Prostate cancer stained with p27 Monoclonal Antibody (KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Immunohistochemistry-Paraffin-NBP2-44495-img0004.jpg)
![Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Western-Blot-NB300-153-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of Neurofibromin 1was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Immunohistochemistry-Paraffin-NB300-153-img0005.jpg)
![Western Blot: APRIN Antibody [NB100-755] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPds5b antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds](http://images.novusbio.com/fullsize/APRIN-Antibody-Western-Blot-NB100-755-img0006.jpg "Western Blot: APRIN Antibody [NB100-755] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPds5b antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds")
![Immunohistochemistry-Paraffin: APRIN Antibody [NB100-755] - Section of human lung small cell carcinoma. Antibody: Affinity purified rabbit anti- Pds5B used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/APRIN-Antibody-Immunohistochemistry-Paraffin-NB100-755-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: APRIN Antibody [NB100-755] - Detection of human Pds5b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pds5b antibody NB100-755 used for IP at 3 ug per reaction. Pds5b was also immunoprecipitated by another rabbit anti-Pds5b antibody. For blotting immunoprecipitated Pds5b, NB100-755 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time time of 75 seconds.](http://images.novusbio.com/fullsize/APRIN-Antibody-Immunoprecipitation-NB100-755-img0007.jpg)
![Western Blot: Hexim 1 Antibody [NBP1-71867] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71868, which recognize other epitopes.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Western-Blot-NBP1-71867-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Hexim 1 Antibody [NBP1-71867] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71868, which recognize other epitopes.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71867-img0009.jpg)
![Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71867-img0008.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NB100-40844] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- KIFC1/MKLP2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-NB100-40844-img0005.jpg "Immunohistochemistry-Paraffin: KIFC1 Antibody [NB100-40844] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- KIFC1/MKLP2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Immunoprecipitation: KIFC1 Antibody [NB100-40844] - Detection of Human KIFC1/MKLP2 on HeLa whole cell lysate using NB100-40844. KIFC1/MKLP2 was also immunoprecipitated by other rabbit anti-KIFC1/MKLP2 antibodies and NB100-40843.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunoprecipitation-NB100-40844-img0003.jpg)
![Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle](http://images.novusbio.com/fullsize/Bmi1-Antibody-Western-Blot-NB110-40823-img0002.jpg "Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle")
![Immunocytochemistry/Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Bmi1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40823-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0006.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (SPM614) [NBP2-44611] - Human Tonsil stained with Nucleolin Monoclonal Antibody (SPM614).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunohistochemistry-Paraffin-NBP2-44611-img0001.jpg)
![Flow Cytometry: Nucleolin Antibody (SPM614) [NBP2-44611] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (SPM614).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Flow-Cytometry-NBP2-44611-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of HeLa cells stained with CF647R labeled Nucleolin Antibody (SPM614)(Green) Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0005.jpg)
![Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-30888-img0008.jpg "Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SET Antibody [NBP1-30888] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SET protein stained by SET antibody, isoform 2-specific diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.](http://images.novusbio.com/fullsize/SET-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30888-img0007.jpg)
![Immunohistochemistry-Paraffin: SET Antibody [NBP1-30888] - A549 xenograft, using SET antibody, isoform 2 specific at 1:100 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SET-Antibody-Immunohistochemistry-Paraffin-NBP1-30888-img0003.jpg)