| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB6000-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34418-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88144-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81406-25ul | |||
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
![Western Blot: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Western blot detection of Cyclin B1 (clone 2061A) catalog number MAB6000 at 0.05 ug/ml in HeLa and Jurkat cell lysates.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Western-Blot-MAB6000-img0002.jpg "Western Blot: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Western blot detection of Cyclin B1 (clone 2061A) catalog number MAB6000 at 0.05 ug/ml in HeLa and Jurkat cell lysates.")
![Immunohistochemistry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Cyclin B1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Cyclin B1 Monoclonal Antibody at 3 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Immunohistochemistry-MAB6000-img0003.jpg)
![Flow Cytometry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - An intracellular stain was performed on HeLa cells with Cyclin B1 Antibody [2061A] MAB6000F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Flow-Cytometry-MAB6000-img0004.jpg)
![Flow Cytometry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - K562 human cell line was stained with Rabbit Anti-Human Cyclin B1 Monoclonal Antibody (Catalog # MAB6000, filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Flow-Cytometry-MAB6000-img0001.jpg)
![Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunoprecipitation-NB100-418-img0006.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-418_rabbit-polyclonal-neurofibromin-1-antibody-western-blot-132202618215117.jpg)
![Neurofibromin loss sensitizes MEF cells to growth factor and matrix stimulation: (a) MEF cells isolated and immortalized from wild-type and knockout mice for Nf1 gene were analyzed by Western blotting with anti-neurofibromin (Nfn) antibodies; (b) focal adhesion kinase FAK binds to Nfn. FAK was immunoprecipitated (IP) from cell lysates of wild-type and knockout MEFs (Nf1+/+, Nf1−/−); following SDS-PAGE electrophoresis, co-immunoprecipitated Nfn were assayed by Western blotting analysis (IB) with anti-FAK and anti-Nfn antibodies. Total protein loaded on the IP is also shown as INPUT ((b), lower panel) and examined by anti-Nfn and anti-actin antibodies; (c) transient phosphorylation kinetics of MEFs stimulated with PDGF-BB (10 ng/mL), [38,39] for increasing time periods. Following SDS-PAGE, cell lysates were incubated with anti-phospho-Erk (1/2) (T202/Y204, T185/Y187) MAP kinase, phospho-FAK (Y397) and phospho-Src (Y416) antibodies and anti-GAPDH; (d,e) colony formation assay of MEFs fully embedded by low growth factor containing Matrigel in absence (upper panel) or presence (lower panel) of insoluble matrix proteins Collagen I/Fibronectin. Cells were seeded in the Matrigel and 24 h later treated or not with PDGF-BB ligand for 8 days (representative microscope pictures (10×)). Where indicated, MEF cells were additionally treated with FAK and MEK inhibitors named Y10 (0.62 μM) and Selumetinib (6.62 μM), respectively; the area of colonies was calculated in five fields for each well, and mean value and SD calculated and plotted in the right histograms. n = 8; * p < 0.05 **; p < 0.001 by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34066061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92100_rabbit-polyclonal-mat2a-antibody-western-blot-13220261739820.jpg)
![Western Blot: TGF-alpha Antibody (SPM542) [NBP2-34371] - analysis of lysate from HEK293 cells (30ug) using anti-TGF alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/TGF-alpha-Antibody-SPM542-Western-Blot-NBP2-34371-img0001.jpg "Western Blot: TGF-alpha Antibody (SPM542) [NBP2-34371] - analysis of lysate from HEK293 cells (30ug) using anti-TGF alpha antibody. Image from verified customer review.")
![Immunohistochemistry-Paraffin: TGF-alpha Antibody (SPM542) [NBP2-34371] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGF alpha Monoclonal Antibody (SPM542)](http://images.novusbio.com/fullsize/TGF-alpha-Antibody-SPM542-Immunohistochemistry-Paraffin-NBP2-34371-img0002.jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0016.jpg "Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of monoclonal antibody to NME2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Immunocytochemistry-Immunofluorescence-H00004831-M06-img0009.jpg)
![Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of monoclonal antibody to NME2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Immunohistochemistry-Paraffin-H00004831-M06-img0011.jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0013.jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0014.jpg)
![Western Blot: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of NME2 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Western-Blot-H00004831-M06-img0015.jpg)
![Sandwich ELISA: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Detection limit for recombinant GST tagged NME2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-1D3-Sandwich-ELISA-H00004831-M06-img0012.jpg)
![Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Western-Blot-H00008850-M04-img0012.jpg "Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in A-431 (Cat # L015V1).")
![Immunocytochemistry/Immunofluorescence: PCAF Antibody (5E11) [H00008850-M04] - Analysis of monoclonal antibody to PCAF on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Immunocytochemistry-Immunofluorescence-H00008850-M04-img0008.jpg)
![Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in LNCaP (Cat # L004V1).](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Western-Blot-H00008850-M04-img0010.jpg)
![Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Western-Blot-H00008850-M04-img0011.jpg)
![Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in human uterus myoma.](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Western-Blot-H00008850-M04-img0013.jpg)
![Sandwich ELISA: PCAF Antibody (5E11) [H00008850-M04] - Detection limit for recombinant GST tagged PCAF is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PCAF-Antibody-5E11-Sandwich-ELISA-H00008850-M04-img0009.jpg)
![Western Blot: SHC1 Antibody (3F4) [H00006464-M01] - SHC1 monoclonal antibody (M01), clone 3F4 Analysis of SHC1 expression in A-431.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Western-Blot-H00006464-M01-img0015.jpg "Western Blot: SHC1 Antibody (3F4) [H00006464-M01] - SHC1 monoclonal antibody (M01), clone 3F4 Analysis of SHC1 expression in A-431.")
![Immunocytochemistry/Immunofluorescence: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of monoclonal antibody to SHC1 on A-431 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Immunocytochemistry-Immunofluorescence-H00006464-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of monoclonal antibody to SHC1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Immunohistochemistry-Paraffin-H00006464-M01-img0013.jpg)
![Proximity Ligation Assay: SHC1 Antibody (3F4) [H00006464-M01] - Protein-protein interactions between STAT5A and SHC1 Mahlavu cells were stained with anti-STAT5A rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Proximity-Ligation-Assay-H00006464-M01-img0007.jpg)
![Proximity Ligation Assay: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of protein-protein interactions between PIK3R1 and SHC1. HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Proximity-Ligation-Assay-H00006464-M01-img0008.jpg)
![Proximity Ligation Assay: SHC1 Antibody (3F4) [H00006464-M01] - Analysis of protein-protein interactions between PIK3R1 and SHC1. Huh7 cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-Proximity-Ligation-Assay-H00006464-M01-img0009.jpg)
![ELISA: SHC1 Antibody (3F4) [H00006464-M01] - Detection limit for recombinant GST tagged SHC1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SHC1-Antibody-3F4-ELISA-H00006464-M01-img0016.jpg)
![Western Blot: CDK5RAP3 Antibody (1G4) [H00080279-M01] - CDK5RAP3 monoclonal antibody (M01), clone 1G4 Analysis of CDK5RAP3 expression in A-431.](http://images.novusbio.com/fullsize/CDK5RAP3-Antibody-1G4-Western-Blot-H00080279-M01-img0010.jpg "Western Blot: CDK5RAP3 Antibody (1G4) [H00080279-M01] - CDK5RAP3 monoclonal antibody (M01), clone 1G4 Analysis of CDK5RAP3 expression in A-431.")
![Immunocytochemistry/Immunofluorescence: CDK5RAP3 Antibody (1G4) [H00080279-M01] - Analysis of monoclonal antibody to CDK5RAP3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CDK5RAP3-Antibody-1G4-Immunocytochemistry-Immunofluorescence-H00080279-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK5RAP3 Antibody (1G4) [H00080279-M01] - Analysis of monoclonal antibody to CDK5RAP3 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/CDK5RAP3-Antibody-1G4-Immunohistochemistry-Paraffin-H00080279-M01-img0008.jpg)
![Sandwich ELISA: CDK5RAP3 Antibody (1G4) [H00080279-M01] - Detection limit for recombinant GST tagged CDK5RAP3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDK5RAP3-Antibody-1G4-Sandwich-ELISA-H00080279-M01-img0009.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0016.jpg "Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Immunocytochemistry-Immunofluorescence-H00002965-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Immunohistochemistry-Paraffin-H00002965-M01-img0011.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 over-expressed 293 cell line, cotransfected with GTF2H1 Validated Chimera RNAi ( Cat # H00002965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 (Cat # H00002965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0012.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5. Analysis of GTF2H1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0014.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Analysis of GTF2H1 expression in Jurkat.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0015.jpg)
![Sandwich ELISA: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Detection limit for recombinant GST tagged GTF2H1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Sandwich-ELISA-H00002965-M01-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: TCP1-eta Antibody [NBP1-88144] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TCP1-eta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88144-img0006.jpg "Immunocytochemistry/Immunofluorescence: TCP1-eta Antibody [NBP1-88144] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human cerebral cortex, gastrointestinal, lymphoid tissues and testis using Anti-NFIA antibody (A) NBP1-81406 shows similar protein distribution across tissues to independent antibody NBP1-81407 (B).](http://images.novusbio.com/fullsize/NFIA-Antibody-Immunohistochemistry-Paraffin-NBP1-81406-img0024.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human cerebral cortex, gastrointestinal, lymphoid tissues and testis using Anti-NFIA antibody (A) NBP1-81406 shows similar protein distribution across tissues to independent antibody NBP1-81407 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: NFIA Antibody [NBP1-81406] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NFIA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81406-img0009.jpg)
![Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/NFIA-Antibody-Immunohistochemistry-Paraffin-NBP1-81406-img0020.jpg)
![Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human gastrointestinal shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NFIA-Antibody-Immunohistochemistry-Paraffin-NBP1-81406-img0021.jpg)
![Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human lymphoid tissues shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/NFIA-Antibody-Immunohistochemistry-Paraffin-NBP1-81406-img0022.jpg)
![Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Staining of human testis shows strong nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/NFIA-Antibody-Immunohistochemistry-Paraffin-NBP1-81406-img0023.jpg)
![Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85278] - Analysis using Anti-DCTN2 antibody NBP1-85278 (A) shows similar pattern to independent antibody NBP1-85277 (B).](http://images.novusbio.com/fullsize/Dynactin-Subunit-2-DCTN2-DCTN-50-Antibody-Western-Blot-NBP1-85278-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85278] - Analysis using Anti-DCTN2 antibody NBP1-85278 (A) shows similar pattern to independent antibody NBP1-85277 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85278] - Staining of human cell line A-431 shows localization to cytosol & centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dynactin-Subunit-2-DCTN2-DCTN-50-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85278-img0009.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85278] - Staining of human colon.](http://images.novusbio.com/fullsize/Dynactin-Subunit-2-DCTN2-DCTN-50-Antibody-Immunohistochemistry-Paraffin-NBP1-85278-img0014.jpg)
![Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85278] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Dynactin-Subunit-2-DCTN2-DCTN-50-Antibody-Western-Blot-NBP1-85278-img0008.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85278] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Dynactin-Subunit-2-DCTN2-DCTN-50-Antibody-Immunohistochemistry-Paraffin-NBP1-85278-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85278] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DCTN2 antibody NBP1-85278 (A) shows similar protein distribution across tissues to independent antibody NBP1-85277 (B).](http://images.novusbio.com/fullsize/Dynactin-Subunit-2-DCTN2-DCTN-50-Antibody-Immunohistochemistry-Paraffin-NBP1-85278-img0010.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85278] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Dynactin-Subunit-2-DCTN2-DCTN-50-Antibody-Immunohistochemistry-Paraffin-NBP1-85278-img0012.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85278] - Staining of human testis.](http://images.novusbio.com/fullsize/Dynactin-Subunit-2-DCTN2-DCTN-50-Antibody-Immunohistochemistry-Paraffin-NBP1-85278-img0013.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85278] - Staining of human kidney.](http://images.novusbio.com/fullsize/Dynactin-Subunit-2-DCTN2-DCTN-50-Antibody-Immunohistochemistry-Paraffin-NBP1-85278-img0015.jpg)