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![Western Blot: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk2.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Western-Blot-NBP2-70392-img0002.jpg "Western Blot: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk2.")
![Immunocytochemistry/Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk2.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70392-img0007.jpg)
![Immunohistochemistry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70392-img0008.jpg)
![Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Cdk2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70392-img0005.jpg)
![Western Blot: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Western-Blot-NBP2-70392-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Staining of HeLa cells using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70392-img0006.jpg)
![Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of Hela cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70392-img0003.jpg)
![Flow Cytometry: CDK2 Antibody (OTI2D9) - Azide and BSA Free [NBP2-70392] - Analysis of Jurkat cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CDK2-Antibody-OTI2D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70392-img0004.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Western-Blot-NBP2-70454-img0002.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunofluorescent staining of HT29 cells using AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70454-img0006.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, 0454)](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70454-img0004.jpg)
![Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells transfected with either RC202718 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454), and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70454-img0008.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with AlphaB Crystallin/CRYAB Antibody (OTI6A9).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Western-Blot-NBP2-70454-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70454-img0005.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP2-70454)](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70454-img0003.jpg)
![Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunoprecipitation (IP) of CRYAB by using a monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunoprecipitation-NBP2-70454-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: VE Cadherin Antibody (BV9) [NB600-1409] - Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV9-Immunocytochemistry-Immunofluorescence-NB600-1409-img0008.jpg "Immunocytochemistry/Immunofluorescence: VE Cadherin Antibody (BV9) [NB600-1409] - Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.")
![Flow Cytometry: VE Cadherin Antibody (BV9) [NB600-1409] - HUVEC cells were stained with antibody BV9 in PBS/0.1% saponin for 1h at 4 degrees C.(Black- no stain, Red- isotype control, Blue-HM2032-BV9)](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV9-Flow-Cytometry-NB600-1409-img0007.jpg)
![Western Blot: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of Calmodulin on rat brain lysates using anti-Calmodulin antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Western-Blot-NBP2-67413-img0001.jpg "Western Blot: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of Calmodulin on rat brain lysates using anti-Calmodulin antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunocytochemistry-Immunofluorescence-NBP2-67413-img0005.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunohistochemistry-Paraffin-NBP2-67413-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunocytochemistry-Immunofluorescence-NBP2-67413-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunocytochemistry-Immunofluorescence-NBP2-67413-img0004.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of paraffin-embedded mouse brain tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-SJ16-09-Immunohistochemistry-Paraffin-NBP2-67413-img0006.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0010.jpg "Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0007.jpg)
![Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Flow-Cytometry-NBP2-67390-img0001.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: A431](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0008.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0003.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0004.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0005.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0006.jpg)
![Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of MKP-1/DUSP1 on SK-BR-3 cell tissue lysates with Rabbit anti-MKP-1/DUSP1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size:39 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Western-Blot-NBP2-67909-img0005.jpg "Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of MKP-1/DUSP1 on SK-BR-3 cell tissue lysates with Rabbit anti-MKP-1/DUSP1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size:39 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of paraffin-embedded mouse lung tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Immunohistochemistry-Paraffin-NBP2-67909-img0003.jpg)
![Flow Cytometry: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of HepG2 cells with DUSP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Flow-Cytometry-NBP2-67909-img0001.jpg)
![Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of DUSP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Jurkat cell lysate Lane 3: mouse spleen tissue lysate](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Western-Blot-NBP2-67909-img0004.jpg)
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of paraffin-embedded human lung cancer tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Immunohistochemistry-Paraffin-NBP2-67909-img0002.jpg)
![Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Western blot analysis of E2F-1 on NIH/3T3 cell lysates with Rabbit anti-E2F1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 47 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Western-Blot-NBP2-67899-img0011.jpg "Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Western blot analysis of E2F-1 on NIH/3T3 cell lysates with Rabbit anti-E2F1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 47 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0004.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0012.jpg)
![Flow Cytometry: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Flow-Cytometry-NBP2-67899-img0001.jpg)
![Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of E2F1 on HepG2 cells lysates using anti-E2F1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Western-Blot-NBP2-67899-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0003.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0005.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0006.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0008.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0009.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0010.jpg)
![Western Blot: STIM1 Antibody (CDN3H4) [NB110-55300] - (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06)](http://images.novusbio.com/fullsize/STIM1-Antibody-CDN3H4-Western-Blot-NB110-55300-img0003.jpg "Western Blot: STIM1 Antibody (CDN3H4) [NB110-55300] - (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06)")
![Immunocytochemistry/Immunofluorescence: STIM1 Antibody (CDN3H4) [NB110-55300] - Staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor(R) 598; red). Nuclei were stained with DAPI (blue).](http://images.novusbio.com/fullsize/STIM1-Antibody-CDN3H4-Immunocytochemistry-Immunofluorescence-NB110-55300-img0004.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G expression in transfected 293T cell line by EEF1G monoclonal antibody (M01), clone 3F11-1A10.Lane 1: EEF1G transfected lysate(50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0016.jpg "Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G expression in transfected 293T cell line by EEF1G monoclonal antibody (M01), clone 3F11-1A10.Lane 1: EEF1G transfected lysate(50 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of monoclonal antibody to EEF1G on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Immunocytochemistry-Immunofluorescence-H00001937-M01-img0008.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G over-expressed 293 cell line, cotransfected with EEF1G Validated Chimera RNAi ( Cat # H00001937-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EEF1G monoclonal antibody (M01), clone 3F11-1A10 (Cat # H00001937-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0009.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in PC-12.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0010.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in HepG2.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0011.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in 293.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0012.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10 Analysis of EEF1G expression in HL-60.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0013.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in Raw 264.7.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0014.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in NIH/3T3.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0015.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - Depletion of TPX2 causes defects in 53BP1 ionizing radiation-induced foci formation. The protein level of 53BP1 is not affected by miRNA-mediated depletion of TPX2. Levels of actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0005.jpg "Western Blot: TPX2 Antibody [NB500-184] - Depletion of TPX2 causes defects in 53BP1 ionizing radiation-induced foci formation. The protein level of 53BP1 is not affected by miRNA-mediated depletion of TPX2. Levels of actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TPX2 Antibody [NB500-184] - Detection of Human TPX2 on HeLa whole cell lysate using NB500-184. TPX2 was also immunoprecipitated using NB500-183 at 0.4 ug/mg lysate.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin and impacts the DAPI staining pattern and H4K16ac levels. TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin and impacts the DAPI staining pattern and H4K16ac levels Depletion of TPX2 by siRNA causes a decrease in H4K16ac levels whereas the levels of H3K9ac and H3K56ac remain unchanged. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Knockdown-Validated-NB500-184-img0004.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin & impacts the DAPI staining pattern & H4K16ac levels.(A) Although the majority of TPX2 is found in the soluble fraction (see Material & Methods), a small but clearly detectable sub-population of TPX2 constitutively associates with stringent chromatin fractions obtained from MCF7 cells (left panel) or HeLa cells (right panel). These chromatin fractions contain histones but not nuclear LaminB. Upon expression of an inducible TPX2 targeting miRNA (or upon transfection with siRNA; see D) the protein was depleted from chromatin fractions. Ctrl: control cells with no induction of TPX2 miRNA. (B) TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. (C) Overexpression of GFP-TPX2 or His-TPX2 causes abnormal DAPI staining in MCF7 cells compared to surrounding non-transfected cells or cells transfected with GFP. This is indicative of changes in chromatin structure. Enlargements of white frames are shown. In agreement with previous reports, overexpressed TPX2 is mostly found in the nucleus but also associates with the cytoskeleton [2]. (D-F) Depletion of TPX2 by siRNA (D) or miRNA (F) causes a decrease in H4K16ac levels whereas the levels of H3K9ac & H3K56ac remain unchanged. (E) Quantification of H3K9ac & H3K56ac levels from MCF7 cells transfected with control or TPX2 siRNA (n = 4 independent experiments each; p(t test)>0.05; NS: non significant; Error bars represent SE). Stripping of western blots & re-development with antibodies specific for H3 & H4 ensured equal loading. See text for details. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416182636.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - SIRT1 modulates the levels of H4K16ac & gamma -H2AX & is in complex with TPX2.(A) siRNA-mediated loss of SIRT1 in HeLa cells increases H4K16ac levels & decreases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (B) Overexpression of SIRT1 in MCF7 cells decreases H4K16ac levels & increases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (C) Co-immunoprecipitations with TPX2 antibodies from HeLa cells with & without ionizing radiation treatment as indicated & in the absence or presence of ethidium bromide (EtBr). (The Input for TPX2 is from a longer exposure of the same blot.) (D) Co-immunoprecipitations with SIRT1 antibodies from HeLa cells with & without ionizing radiation treatment as indicated. Beads without antibodies (No Ab) were used as controls. Cells were treated with 10 Gy (or left untreated) & harvested after 1h recovery (A-B) or as indicated (C-D). See text for details. Levels of H2AX & H4 were used as loading controls. IR: ionizing radiation. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416161844.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - SIRT1 modulates the levels of H4K16ac & gamma -H2AX & is in complex with TPX2.(A) siRNA-mediated loss of SIRT1 in HeLa cells increases H4K16ac levels & decreases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (B) Overexpression of SIRT1 in MCF7 cells decreases H4K16ac levels & increases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (C) Co-immunoprecipitations with TPX2 antibodies from HeLa cells with & without ionizing radiation treatment as indicated & in the absence or presence of ethidium bromide (EtBr). (The Input for TPX2 is from a longer exposure of the same blot.) (D) Co-immunoprecipitations with SIRT1 antibodies from HeLa cells with & without ionizing radiation treatment as indicated. Beads without antibodies (No Ab) were used as controls. Cells were treated with 10 Gy (or left untreated) & harvested after 1h recovery (A-B) or as indicated (C-D). See text for details. Levels of H2AX & H4 were used as loading controls. IR: ionizing radiation. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416171433.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin & impacts the DAPI staining pattern & H4K16ac levels.(A) Although the majority of TPX2 is found in the soluble fraction (see Material & Methods), a small but clearly detectable sub-population of TPX2 constitutively associates with stringent chromatin fractions obtained from MCF7 cells (left panel) or HeLa cells (right panel). These chromatin fractions contain histones but not nuclear LaminB. Upon expression of an inducible TPX2 targeting miRNA (or upon transfection with siRNA; see D) the protein was depleted from chromatin fractions. Ctrl: control cells with no induction of TPX2 miRNA. (B) TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. (C) Overexpression of GFP-TPX2 or His-TPX2 causes abnormal DAPI staining in MCF7 cells compared to surrounding non-transfected cells or cells transfected with GFP. This is indicative of changes in chromatin structure. Enlargements of white frames are shown. In agreement with previous reports, overexpressed TPX2 is mostly found in the nucleus but also associates with the cytoskeleton [2]. (D-F) Depletion of TPX2 by siRNA (D) or miRNA (F) causes a decrease in H4K16ac levels whereas the levels of H3K9ac & H3K56ac remain unchanged. (E) Quantification of H3K9ac & H3K56ac levels from MCF7 cells transfected with control or TPX2 siRNA (n = 4 independent experiments each; p(t test)>0.05; NS: non significant; Error bars represent SE). Stripping of western blots & re-development with antibodies specific for H3 & H4 ensured equal loading. See text for details. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416173534.jpg)
![Western Blot: Rad50 Antibody [NB100-1487] - Detection of Rad50 using NB100-1487. Lane 1: 25 ng purified hMre11-hRad50 complex Lane 2: 25 ug crude HeLa extract.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NB100-1487-img0003.jpg "Western Blot: Rad50 Antibody [NB100-1487] - Detection of Rad50 using NB100-1487. Lane 1: 25 ng purified hMre11-hRad50 complex Lane 2: 25 ug crude HeLa extract.")
![Simple Western: Rad50 Antibody [NB100-1487] - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Rad50-Antibody-Simple-Western-NB100-1487-img0004.jpg)
![Western Blot: DLL3 Antibody [NBP2-24669] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/mL respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DLL3-Antibody-Western-Blot-NBP2-24669-img0001.jpg "Western Blot: DLL3 Antibody [NBP2-24669] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/mL respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: DLL3 Antibody [NBP2-24669] - Human brain tissue probed with an isotype control (top) and DLL3 antibody (bottom) at 5 ug/mL.](http://images.novusbio.com/fullsize/DLL3-Antibody-Immunohistochemistry-Paraffin-NBP2-24669-img0002.jpg)