| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78783SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90103-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87592-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89480-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87757-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86928-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59451-50ul | |||
![Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP2-78783] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti-Tensin 1 at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved. Note the localization of Tensin1 to focal adhesion sites.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78783-img0004.jpg "Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP2-78783] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti-Tensin 1 at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved. Note the localization of Tensin1 to focal adhesion sites.")
![Western Blot: Tensin 1 Antibody [NBP2-78783] - Total protein from human U2OS and HeLa cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Tensin1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Western-Blot-NBP2-78783-img0003.jpg)
![Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP2-78783] - Analysis of a FFPE tissue section of the human kidney using 1:200 dilution of Tensin1 antibody (NBP2-78783). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly strong cytoplasmic staining in all cells in glomeruli and tubules.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-78783-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP2-78783] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tensin1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78783-img0001.jpg)
![Western Blot: Aurora A Antibody [NBP1-90103] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP1-90103-img0007.jpg "Western Blot: Aurora A Antibody [NBP1-90103] - Analysis in human cell line SK-BR-3.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-90103] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, cytosol & centrosome.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90103-img0009.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0014.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human duodenum shows weak to moderate cytoplasmic positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0011.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0012.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0013.jpg)
![Western Blot: Cyclin T2 Antibody [NBP1-87592] - Cyclin T1 (Ccnt1) gene is required for ER stress-mediated CHOP induction and vascular calcification. Immunoblot analysis of CDK9, cyclin T1, cyclin T2, cyclin K, ATF4, CHOP, and GAPDH proteins in BSA (Veh) or 500 um C18:0 treated scrambled (Scramb.) sg or Ccnt1 KO VSMCs for 6 h. Image collected and cropped by CiteAb from the following publication (//www.jbc.org/lookup/doi/10.1074/jbc.RA118.004706), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cyclin-T2-Antibody-Western-Blot-NBP1-87592-img0007.jpg "Western Blot: Cyclin T2 Antibody [NBP1-87592] - Cyclin T1 (Ccnt1) gene is required for ER stress-mediated CHOP induction and vascular calcification. Immunoblot analysis of CDK9, cyclin T1, cyclin T2, cyclin K, ATF4, CHOP, and GAPDH proteins in BSA (Veh) or 500 um C18:0 treated scrambled (Scramb.) sg or Ccnt1 KO VSMCs for 6 h. Image collected and cropped by CiteAb from the following publication (//www.jbc.org/lookup/doi/10.1074/jbc.RA118.004706), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Cyclin T2 Antibody [NBP1-87592] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.](http://images.novusbio.com/fullsize/Cyclin-T2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87592-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin T2 Antibody [NBP1-87592] - Staining of human duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclin-T2-Antibody-Immunohistochemistry-Paraffin-NBP1-87592-img0005.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Cyclin T2 Antibody [NBP1-87592] - Cyclin T2 (Ccnt2) and cyclin K (Ccnk) deficiency induces CHOP expression and calcification, opposite of cyclin T1 deficiency. Immunoblot analysis of CDK9, cyclin T1, cyclin T2, cyclin K, ATF4, CHOP, and GAPDH proteins in BSA (Veh) or 500 um C18:0 treated scrambled (Scramb.) sg or Ccnt2 KO VSMCs for 6 h. Image collected and cropped by CiteAb from the following publication (//www.jbc.org/lookup/doi/10.1074/jbc.RA118.004706), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cyclin-T2-Antibody-Knockout-Validated-NBP1-87592-img0006.jpg)
![Western Blot: Cyclin T2 Antibody [NBP1-87592] - Cyclin T2 (Ccnt2) & cyclin K (Ccnk) deficiency induces CHOP expression & calcification, opposite of cyclin T1 deficiency.A, immunoblot analysis of CDK9, cyclin T1, cyclin T2, cyclin K, ATF4, CHOP, & GAPDH proteins in BSA (Veh) or 500 μm C18:0 treated scrambled (Scramb.) sg or Ccnt2 KO VSMCs for 6 h. B, qRT-PCR analysis of CHOP in BSA (Veh) or 250 μm C18:0 treated Scramb. sg or Ccnt2 KO VSMCs (n = 4). C, immunoblot analysis of CDK9, cyclin T1, cyclin T2, cyclin K, ATF4, CHOP, & GAPDH proteins in BSA (Veh) or 500 μm C18:0 treated Scramb. sg or Ccnk heterozygous KO VSMCs for 6 h. D, qRT-PCR analysis of CHOP in BSA (Veh) or 250 μm C18:0 treated Scramb. sg or Ccnk heterozygous KO VSMCs (n = 4). E, mineralization of Ccnt2 KO VSMCs. VSMCs were incubated with 2.0 mm phosphate with or without 250 μm C18:0 for 7 days (n = 6). F, mineralization of Ccnk hetero-KO VSMCs. VSMCs were incubated with 2.0 mm phosphate with or without 250 μm C18:0 for 7 days (n = 6). One-way ANOVA with a Student-Newman post hoc test was used for statistical analysis. *, p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30209133), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87592_rabbit-polyclonal-cyclin-t2-antibody-310202416114781.jpg)
![Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Analysis in human testis and pancreas tissues. Corresponding PPHLN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PPHLN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89480-img0030.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Analysis in human testis and pancreas tissues. Corresponding PPHLN1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human cerebral cortex, colon, liver and testis using Anti-MAPRE2 antibody (A) NBP1-89480 shows similar protein distribution across tissues to independent antibody NBP2-38431 (B).](http://images.novusbio.com/fullsize/PPHLN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89480-img0028.jpg)
![Western Blot: PPHLN1 Antibody [NBP1-89480] - Human foreskin fibroblasts whole cell lysates. Antibody at 1:1000. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PPHLN1-Antibody-Western-Blot-NBP1-89480-img0031.jpg)
![Immunocytochemistry/Immunofluorescence: PPHLN1 Antibody [NBP1-89480] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PPHLN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89480-img0012.jpg)
![Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/PPHLN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89480-img0023.jpg)
![Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PPHLN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89480-img0024.jpg)
![Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human liver shows weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/PPHLN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89480-img0025.jpg)
![Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human pancreas shows negative to very weak positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/PPHLN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89480-img0026.jpg)
![Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PPHLN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89480-img0027.jpg)
![Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Western-Blot-NBP1-87757-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: NF2/Merlin Antibody [NBP1-87757] - Whole cell lysates from SUM159, MDA-MB-231 and MCF-7 cells were loaded with 50 ug/lane. 10% SDS-PAGE. NF2/Merlin Antibody (NBP1-87757) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Western-Blot-NBP1-87757-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NF2/Merlin Antibody [NBP1-87757] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87757-img0008.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - Staining of human fallopian tube shows moderate cytoplasmic and moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0011.jpg)
![Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Western-Blot-NBP1-87757-img0007.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0001.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0009.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0010.jpg)
![Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Western-Blot-NBP1-86928-img0016.jpg "Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Immunohistochemistry-Paraffin-NBP1-86928-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NFIX Antibody [NBP2-15039] - Nfix is mainly expressed by anti-inflammatory MPs. Percentage of F4/80+ MPs positive for Nfix in Tibialis Anterior muscles (TA) of WT mice injected by CTX at D2, D4 and D7, post-injury. Immunostaining for F4/80 (green), Nfix (red) and DAPI (blue) at D4 and D7 after CTX injection. Results are means +/- SEM of at least three independent experiments. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/9/3/708) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NFIX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15039-img0010.jpg "Immunocytochemistry/Immunofluorescence: NFIX Antibody [NBP2-15039] - Nfix is mainly expressed by anti-inflammatory MPs. Percentage of F4/80+ MPs positive for Nfix in Tibialis Anterior muscles (TA) of WT mice injected by CTX at D2, D4 and D7, post-injury. Immunostaining for F4/80 (green), Nfix (red) and DAPI (blue) at D4 and D7 after CTX injection. Results are means +/- SEM of at least three independent experiments. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/9/3/708) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] - Mouse hippocampus.NFIX stained by NFIX antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-15039_rabbit-polyclonal-nfix-antibody-immunohistochemistry-paraffin-282023113015..jpg)
![Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] - Mouse hippocampus.NFIX stained by NFIX antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-15039_rabbit-polyclonal-nfix-antibody-immunohistochemistry-paraffin-282023113118..jpg)
![Western Blot: p27/Kip1 Antibody [NBP1-32213] - Wild-type (WT) and p27 Kip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Western-Blot-NBP1-32213-img0030.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p27/Kip1 Antibody [NBP1-32213] - Wild-type (WT) and p27 Kip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody [NBP1-32213] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p27 Kip1 protein stained by p27 Kip1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32213-img0016.jpg)
![Immunohistochemistry-Frozen: p27/Kip1 Antibody [NBP1-32213] - Frozen sectioned adult mouse retina. Green: p27 Kip1 protein stained by p27 Kip1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Immunohistochemistry-Frozen-NBP1-32213-img0019.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: p27/Kip1 Antibody [NBP1-32213] - Wild-type (WT) and p27 Kip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Western-Blot-NBP1-32213-img0029.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NBP1-32213] - Staining of human ulcerative colitis tissue using anti-p27 Kip1 antibody.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Immunohistochemistry-Frozen-NBP1-32213-img0011.jpg)
![Immunoprecipitation: p27/Kip1 Antibody [NBP1-32213] - Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using 5 ug of p27 Kip1 antibody. Western blot analysis was performed using p27 Kip1 antibody.EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Immunoprecipitation-NBP1-32213-img0023.jpg)
![Western Blot: p27/Kip1 Antibody [NBP1-32213] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (NBP1-32213) diluted at 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp1-32213_rabbit-polyclonal-p27-kip1-antibody-western-blot-512024112649..jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: p27/Kip1 Antibody [NBP1-32213] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32213_rabbit-polyclonal-p27-kip1-antibody-1610202419405788.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrinogen beta chain Antibody [NBP1-33582] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33582-img0005.jpg "Immunocytochemistry/Immunofluorescence: Fibrinogen beta chain Antibody [NBP1-33582] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-33582] - Rat liver. Fibrinogen beta antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Immunohistochemistry-Paraffin-NBP1-33582-img0008.jpg)
![Western Blot: Fibrinogen beta chain Antibody [NBP1-33582] - Mouse tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta chain antibody diluted at 1:50000.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Western-Blot-NBP1-33582-img0009.jpg)
![Western Blot: Fibrinogen beta chain Antibody [NBP1-33582] - Rat tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta chain antibody diluted at 1:50000.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Western-Blot-NBP1-33582-img0010.jpg)
![Immunohistochemistry: Rabbit Polyclonal Fibrinogen beta chain Antibody [NBP1-33582] - Staining of mouse C57BL/6 retina. Fibrinogen beta chain (red) colocalized with BS-1 lectin (cyan) labelled retinal vasculature. Primary antibody dilution: 1:100 dilution. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-33582_rabbit-polyclonal-fibrinogen-beta-chain-antibody-immunohistochemistry-1552024171234.png)
![Western Blot: Fibrinogen beta chain Antibody [NBP1-33582] - Human plasma (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta chain antibody [C1C3] (NBP1-33582) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33582_rabbit-polyclonal-fibrinogen-beta-chain-antibody-91020242091451.jpg)
![Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched 1-PTCH Antibody - BSA Free-Western Blot-NB200-118-img0004.jpg "Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Evaluation of IGF-1, IGF1R and HH pathway expression in fibroblasts and oral squamous carcinoma cells. IGF1 mRNA expression in SCC-4 and MF1 cells, evaluated by RT-qPCR. (C) Evaluation of HH pathway component expression in MF1 by immunocytochemistry. Patched 1/PTCH Antibody (NB200-118); Sonic Hedgehog/Shh Antibody (NBP2-22126). Magnification: 400x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB200-118-img0003.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Increased Ihh mRNA and protein levels and activity in Smad2 and Smad3 mutant growth plates.All images are P0 proximal tibias. Immunostaining for Ptch1, showing increased protein levels throughout the growth plate in mutants, particularly in the resting zone. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006352) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Immunohistochemistry-NB200-118-img0002.jpg)
![Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Western-Blot-NB200-118-img0001.jpg)
![Western Blot: Jak2 Antibody (53B7) [NBP2-59451] - Human breast cancer MCF7 cells were treated with control (Con) or Adenosine (Ado) and the expression of JAK2 was detected. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Jak2-Antibody-53B7-Western-Blot-NBP2-59451-img0004.jpg "Western Blot: Jak2 Antibody (53B7) [NBP2-59451] - Human breast cancer MCF7 cells were treated with control (Con) or Adenosine (Ado) and the expression of JAK2 was detected. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: Jak2 Antibody (53B7) [NBP2-59451] - Analysis of JAK2 in HeLa cells line, stained with DAPI (Blue) and anti-human JAK2 antibody at 1:100 with goat anti-mouse IgG Alexa Fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Jak2-Antibody-53B7-Immunocytochemistry-Immunofluorescence-NBP2-59451-img0002.jpg)
![Flow Cytometry: Jak2 Antibody (53B7) [NBP2-59451] - Analysis of JAK2 in HeLa cell line, antibody at 2 - 5 ug per 10^6cells (red line). Secondary antibody: goat anti-mouse IgG Alexa Fluor 488 conjugate. Isotype control antibody: mouse IgG (black line).](http://images.novusbio.com/fullsize/Jak2-Antibody-53B7-Flow-Cytometry-NBP2-59451-img0003.jpg)
![Western Blot: Jak2 Antibody (53B7) [NBP2-59451] - Lane 1: K-562 cell lysates, Lane 2: TF-1 cell lysates, Lane 3: 293T cell lysates, Lane 4: Jurkat cell lysates, Lane 5: THP-1 cell lysates](http://images.novusbio.com/fullsize/Jak2-Antibody-53B7-Western-Blot-NBP2-59451-img0001.jpg)
![Microarray: Jak2 Antibody (53B7) [NBP2-59451] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/Jak2-Antibody-53B7-Microarray-NBP2-59451-img0005.jpg)
![Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Western-Blot-NB100-59828-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunohistochemistry-NB100-59828-img0010.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human CDC20 on HeLa whole cell lysate using NB100-59828. CDC20 was also immunoprecipitated by rabbit anti-CDC20 antibody NB100-59827.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Western-Blot-NB100-59828-img0006.jpg)
![Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunohistochemistry-NB100-59828-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Cdc20 Antibody [NB100-59828] - Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59828 (lot NB100-59828-2) used for IP at 6 ug per reaction. CDC20 was also immunoprecipitated by a previous lot of this antibody (lot NB100-59828-1) and rabbit anti-CDC20 antibody NB100-59827. For blotting immunoprecipitated CDC20, NB100-59828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunoprecipitation-NB100-59828-img0013.jpg)