| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22450-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27345SS | |||
| Azide and BSA Free | NBP2-80793 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90207-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81796-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43943 | |||
| BSA Free | NBP2-61616-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48733-25ul | |||
![Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts of various cell lines, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Western-Blot-NBP3-15854-img0003.jpg "Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts of various cell lines, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts from normal (control) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Knockout-Validated-NBP3-15854-img0001.jpg)
![Immunoprecipitation: ATG5 Antibody (8L7X6) [NBP3-15854] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG5 antibody (NBP3-15854). Western blot was performed from the immunoprecipitate using ATG5 (NBP3-15854) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Immunoprecipitation-NBP3-15854-img0004.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251726014.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517251919.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251720294.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251726015.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517273524.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Human colon cancer tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517213515.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-522025171896.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517284996.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded rat colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517152727.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517172826.jpg)
![Western Blot: HCN1 Antibody (S70) [NBP1-22450] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Western-Blot-NBP1-22450-img0011.jpg "Western Blot: HCN1 Antibody (S70) [NBP1-22450] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Neurons.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0014.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Human Hippocampus](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0001.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0012.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0013.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0015.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0008.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Intracellular staining of Interleukin-1 beta positive T cells (upper panel) & monocytes (lower panel) from stimulated PBMC (with a cocktail of TLR ligands and PMA/IM for 6h) using Interleukin-1 beta antibody at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Flow-Cytometry-NBP2-27345-img0006.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of full length human IL-1 beta recombinant protein (10ng/well) using IL1 beta Antibody (NBP2-27345) at 1:1000 (left lane) and 1:100 (right lane).](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0009.jpg)
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in human kidney tissue using Interleukin-1 beta antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Immunohistochemistry-Paraffin-NBP2-27345-img0007.jpg)
![SDS-Page: Recombinant Human NLRP3/NALP3 Protein [H00114548-P01] - 12.5% SDS-PAGE stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-NLRP3-NALP3-Protein-SDS-Page-H00114548-P01-img0002.jpg "SDS-Page: Recombinant Human NLRP3/NALP3 Protein [H00114548-P01] - 12.5% SDS-PAGE stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human tonsil shows moderate cytoplasmic positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0011.jpg "Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human tonsil shows moderate cytoplasmic positivity in non - germinal center cells.")
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0008.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0009.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human spleen shows cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0010.jpg)
![Western Blot: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA by WB of Jurkat, MOLT-4, MJ, RPMI-8226, HeLa, KG-1, HEK293T, and SUP-M2 lysate. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406). Secondary: HRP-conjugated goat anti-rabbit IgG.Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Western-Blot-NBP2-76406-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA (red) in FFPE ovarian carcinoma by IHC-IF. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] NBP2-76406. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunocytochemistry-Immunofluorescence-NBP2-76406-img0004.jpg)
![Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunohistochemistry-NBP2-76406-img0002.jpg)
![Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Rabbit anti-VISTA recombinant monoclonal [BLR035F] (right) or isotype control (left). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Flow-Cytometry-NBP2-76406-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA in FFPE NCI-H226 cells by ICC. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunocytochemistry-NBP2-76406-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA by WB of immunoprecipitates from Jurkat lysate. Antibodies: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406) and another rabbit anti-VISTA recombinant monoclonal.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunoprecipitation-NBP2-76406-img0006.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![Western Blot: LANCL1 Antibody [NBP1-81796] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/LANCL1-Antibody-Western-Blot-NBP1-81796-img0013.jpg "Western Blot: LANCL1 Antibody [NBP1-81796] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: LANCL1 Antibody [NBP1-81796] - Immunofluorescent staining of human cell line A-431 shows localization to microtubules.](http://images.novusbio.com/fullsize/LANCL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81796-img0007.jpg)
![Immunohistochemistry-Paraffin: LANCL1 Antibody [NBP1-81796] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons and neuropil.](http://images.novusbio.com/fullsize/LANCL1-Antibody-Immunohistochemistry-Paraffin-NBP1-81796-img0011.jpg)
![Western Blot: LANCL1 Antibody [NBP1-81796] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/LANCL1-Antibody-Western-Blot-NBP1-81796-img0012.jpg)
![Immunohistochemistry-Paraffin: LANCL1 Antibody [NBP1-81796] - Staining of human fallopian tube shows moderate positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/LANCL1-Antibody-Immunohistochemistry-Paraffin-NBP1-81796-img0008.jpg)
![Immunohistochemistry-Paraffin: LANCL1 Antibody [NBP1-81796] - Staining of human kidney shows weak membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/LANCL1-Antibody-Immunohistochemistry-Paraffin-NBP1-81796-img0009.jpg)
![Immunohistochemistry-Paraffin: LANCL1 Antibody [NBP1-81796] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/LANCL1-Antibody-Immunohistochemistry-Paraffin-NBP1-81796-img0010.jpg)
![Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HMGCR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0259-Western-Blot-NBP2-61616-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HMGCR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line MCF-7 Lane 3: Human cell line HepG2](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0259-Western-Blot-NBP2-61616-img0008.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0259-Immunohistochemistry-Paraffin-NBP2-61616-img0007.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human liver shows strong cytoplasmic immunoreactivity.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0259-Immunohistochemistry-Paraffin-NBP2-61616-img0002.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human breast cancer shows moderate cytoplasmic immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0259-Immunohistochemistry-Paraffin-NBP2-61616-img0003.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0259-Immunohistochemistry-Paraffin-NBP2-61616-img0004.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human cervix shows cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0259-Immunohistochemistry-Paraffin-NBP2-61616-img0005.jpg)
![Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP2-61616] - Staining of human liver cancer shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/HMG-CoA-Reductase-HMGCR-Antibody-CL0259-Immunohistochemistry-Paraffin-NBP2-61616-img0006.jpg)
![Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human cerebral cortex, colon, liver and testis using Anti-CYP51A1 antibody NBP2-48733 (A) shows similar protein distribution across tissues to independent antibody NBP2-48793 (B).](http://images.novusbio.com/fullsize/CYP51A1-Antibody-Immunohistochemistry-Paraffin-NBP2-48733-img0008.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human cerebral cortex, colon, liver and testis using Anti-CYP51A1 antibody NBP2-48733 (A) shows similar protein distribution across tissues to independent antibody NBP2-48793 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: CYP51A1 Antibody [NBP2-48733] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CYP51A1-Antibody-Western-Blot-NBP2-48733-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CYP51A1 Antibody [NBP2-48733] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CYP51A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48733-img0004.jpg)
![Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human testis using Anti-CYP51A1 antibody NBP2-48733.](http://images.novusbio.com/fullsize/CYP51A1-Antibody-Immunohistochemistry-Paraffin-NBP2-48733-img0012.jpg)
![Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/CYP51A1-Antibody-Immunohistochemistry-Paraffin-NBP2-48733-img0002.jpg)
![Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/CYP51A1-Antibody-Immunohistochemistry-Paraffin-NBP2-48733-img0007.jpg)
![Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/CYP51A1-Antibody-Immunohistochemistry-Paraffin-NBP2-48733-img0009.jpg)
![Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human liver.](http://images.novusbio.com/fullsize/CYP51A1-Antibody-Immunohistochemistry-Paraffin-NBP2-48733-img0010.jpg)
![Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human colon.](http://images.novusbio.com/fullsize/CYP51A1-Antibody-Immunohistochemistry-Paraffin-NBP2-48733-img0011.jpg)