| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-502SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44934 | |||
| Azide and BSA Free | NBP2-34656-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56339SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24662SS | |||
![Western Blot: NCOA2 Antibody [NB100-388] - Detection of Mouse NCOA2/ SRC2 by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 antibody NB100-388 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NCOA2-Antibody-Western-Blot-NB100-388-img0002.jpg "Western Blot: NCOA2 Antibody [NB100-388] - Detection of Mouse NCOA2/ SRC2 by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 antibody NB100-388 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: NCOA2 Antibody [NB100-388] - Detection of human NCOA2/ SRC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa(15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 antibody NB100-388 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRC2 was also immunoprecipitated by a previous lot (lot 1) and by rabbit anti-NCOA1/SRC2 antibody NBP2-22162, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/NCOA2-Antibody-Immunoprecipitation-NB100-388-img0003.jpg)
![Western Blot: FANCD2 [p Ser222] Antibody [NB100-502] - Indicated lymphoblasts (PD7, WT: GM1526, AT) were irradiated with 15 Gy (2), and immunoblotted with anti-FANCD2 and anti-FANCD2 (pS-222)](http://images.novusbio.com/fullsize/FANCD2-[p-Ser222]-Antibody-Western-Blot-NB100-502-img0007.jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: FANCD2 [p Ser222] Antibody [NB100-502] - Indicated lymphoblasts (PD7, WT: GM1526, AT) were irradiated with 15 Gy (2), and immunoblotted with anti-FANCD2 and anti-FANCD2 (pS-222)")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: FANCD2 [p Ser222] Antibody [NB100-502] - FA-D2 fibroblasts were stably transfected with either pMMP (empty vector, A), FANCD2 (wt, B), FANCD2 [p Ser222] Antibody (C), FANCD2 (triple mutant, D), or FANCD2 (quadruple mutant, E).](http://images.novusbio.com/fullsize/FANCD2-[p-Ser222]-Antibody-Western-Blot-NB100-502-img0006.jpg)
![Western Blot: Cytokeratin 8 Antibody (K8/383) [NBP2-34266] - Western Blot Analysis of A431 cell Lysate using Cytokeratin 8 Monoclonal Antibody (K8/383).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-383-Western-Blot-NBP2-34266-img0004.jpg "Western Blot: Cytokeratin 8 Antibody (K8/383) [NBP2-34266] - Western Blot Analysis of A431 cell Lysate using Cytokeratin 8 Monoclonal Antibody (K8/383).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (K8/383) [NBP2-34266] - Confocal immunofluorescence image of HeLa cells using Cytokeratin 8 Antibody (K8/383). Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-383-Immunocytochemistry-Immunofluorescence-NBP2-34266-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (K8/383) [NBP2-34266] - Formalin-fixed, paraffin-embedded human colon stained with Cytokeratin 8 MAb (K8/383).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-383-Immunohistochemistry-Paraffin-NBP2-34266-img0001.jpg)
![Flow Cytometry: Cytokeratin 8 Antibody (K8/383) [NBP2-34266] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 8 Antibody (K8/383).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-383-Flow-Cytometry-NBP2-34266-img0005.jpg)
![Simple Western: Cytokeratin 8 Antibody (K8/383) [NBP2-34266] - Simple Western lane view shows a specific band for Cytokeratin 8 in 0.2 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-383-Simple-Western-NBP2-34266-img0002.jpg)
![Simple Western: Cytokeratin 8 Antibody (K8/383) [NBP2-34266] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 8 antibody was used at 10 ug/ml dilution of MCF-7 lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-K8-383-Simple-Western-NBP2-34266-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP2-19860] - Plastin L antibody detects Plastin L protein by immunofluorescent analysis. Sample: A549 cells were fixed in ice-cold MeOH for 5 min. Green: Plastin Lstained by Plastin L antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Plastin-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19860-img0007.jpg "Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP2-19860] - Plastin L antibody detects Plastin L protein by immunofluorescent analysis. Sample: A549 cells were fixed in ice-cold MeOH for 5 min. Green: Plastin Lstained by Plastin L antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.")
![Western Blot: Plastin L Antibody [NBP2-19860] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Plastin L antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Plastin-L-Antibody-Western-Blot-NBP2-19860-img0008.jpg)
![Immunohistochemistry-Paraffin: Plastin L Antibody [NBP2-19860] - Plastin L antibody detects Plastin L protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.Plastin L stained by Plastin L antibody (NBP2-19860) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19860_rabbit-polyclonal-plastin-l-antibody-91020242063320.jpg)
![Western Blot: TET1 Antibody [NBP2-19290] - WB analysis of lysates from 293T cell which were mock transfected (lane1) or with tagged-mTet1 (lane 2) for 24hrs, using either TET1 antibody (diluted at 1:2000) (A) or anti-tag (B) antibody.](http://images.novusbio.com/fullsize/TET1-Antibody-Western-Blot-NBP2-19290-img0003.jpg "Western Blot: TET1 Antibody [NBP2-19290] - WB analysis of lysates from 293T cell which were mock transfected (lane1) or with tagged-mTet1 (lane 2) for 24hrs, using either TET1 antibody (diluted at 1:2000) (A) or anti-tag (B) antibody.")
![Western Blot: TET1 Antibody [NBP2-19290] - WB validation of TET1 antibody on 30 ug whole cell extracts of non-transfected (-) and mouse TET1-transfected (+, including DDDDK-tag) 293T cells. The proteins were separated on 5% SDS-PAGE, and the membrane was blotted with TET1 antibody at a dilution of 1:20000.](http://images.novusbio.com/fullsize/TET1-Antibody-Western-Blot-NBP2-19290-img0001.jpg)
![TET1 antibody [N1], N-term detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.TET1 stained by TET1 antibody [N1], N-term (NBP2-19290) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19290_rabbit-polyclonal-tet1-antibody-immunohistochemistry-paraffin-261120251838404.jpg)
![Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 103kDa/ 103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms 70 and 40kDa.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Western-Blot-NBP1-44092-img0003.jpg "Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 103kDa/ 103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms 70 and 40kDa.")
![Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15334-img0001.jpg "Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Cal27 xenograft, using ALDH1A1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP2-15334-img0002.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Western-Blot-NBP2-15334-img0003.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE ALDH1A1 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP2-15334-img0006.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15334] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP2-15334) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15334_rabbit-polyclonal-aldehyde-dehydrogenase-1-a1-aldh1a1-antibody-9102024206330.jpg)
![Western Blot: Mre11 Antibody [NB100-56339] - Western blot analysis of 293 lysate blotted with MRE11 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Mre11-Antibody-Western-Blot-NB100-56339-img0003.jpg "Western Blot: Mre11 Antibody [NB100-56339] - Western blot analysis of 293 lysate blotted with MRE11 antibody at 2 ug/ml.")
![Immunohistochemistry-Paraffin: Mre11 Antibody [NB100-56339] - Paraffin-embedded human testis using MRE11 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Mre11-Antibody-Immunohistochemistry-Paraffin-NB100-56339-img0002.jpg)
![Western Blot: GLI-1 Antibody [NBP2-24662] - Analysis of human kidney lysate using 1) pre-immune sera at 1:5000 dilution and 2) GLI-1 antibody at 1 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Western-Blot-NBP2-24662-img0006.jpg "Western Blot: GLI-1 Antibody [NBP2-24662] - Analysis of human kidney lysate using 1) pre-immune sera at 1:5000 dilution and 2) GLI-1 antibody at 1 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody [NBP2-24662] - staining of GLI-1 at 10 ug/ml. Top panel: C666-1 malignant human nasopharyngel carcinoma cell line. Bottom panel: OKF6 tert-1 human oral epithelial cell line. GLI-1 was more highly expressed in the C666-1 than in the OKF6 tert-1 cells. See application notes for protocol details.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24662-img0004.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody [NBP2-24662] - Formalin-fixed, paraffin-embedded human brain tissue stained with an isotype control antibody (top) and GLI-1 antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24662-img0005.jpg)
![Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Section of human prostate carcinoma. Affinity purified rabbit anti-Emsy used at a dilution of 1:100 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0015.jpg)
![Western Blot: EMSY Antibody [NB100-545] - Nuclear extract (NE) from HeLa cells.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0010.jpg)
![Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-EMSY used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: EMSY Antibody [NB100-545] - Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 (lot NB100-545-2) used for IP at 6 ug/mg lysate. EMSY was also immunoprecipitated by a previous lot of this antibody (lot NB100-545-1) and another rabbit anti-EMSY antibody. For blotting immunoprecipitated EMSY, NB100-545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunoprecipitation-NB100-545-img0014.jpg)
![Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP2-36737-img0001.jpg "Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.")
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP2-36737] - Analysis of FFPE section of human testis using Aurora A antibody at 10 ug/ml concentration. Heat-induced antigen retrieval was employed in this IHC-P validation.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP2-36737-img0002.jpg)
![Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP2-24679-img0002.jpg "Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.")
![Immunohistochemistry-Paraffin: IFRD1 Antibody [NBP2-24679] - Immunohistochemical analysis of IFRD1 in formalin-fixed, paraffin-embedded humanrectum tissue using an isotype control (top) and NBP2-24679 (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Immunohistochemistry-Paraffin-NBP2-24679-img0001.jpg)