| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54576-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59619 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31146SS | |||
| Azide and BSA Free | NBP2-80886 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB500-182SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25269 | |||
![Western Blot: NDUFB5 Antibody [NBP1-31684] - A. 30 ug 293T whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Western-Blot-NBP1-31684-img0005.jpg "Western Blot: NDUFB5 Antibody [NBP1-31684] - A. 30 ug 293T whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: NDUFB5 Antibody [NBP1-31684] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31684-img0002.jpg)
![Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684] - NDUFB6 antibody detects NDUFB6 protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. NDUFB6 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunohistochemistry-Paraffin-NBP1-31684-img0007.jpg)
![Western Blot: NDUFB5 Antibody [NBP1-31684] - 50 ug mouse brain lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Western-Blot-NBP1-31684-img0004.jpg)
![Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684] - Paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunohistochemistry-Paraffin-NBP1-31684-img0003.jpg)
![Immunohistochemistry-Frozen (Negative): NDUFB5 Antibody [NBP1-31684] - NDUFB5 antibody detects NDUFB5 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NDUFB5 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunohistochemistry-Frozen-Negative-NBP1-31684-img0006.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded mouse brain; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0012.jpg "Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded mouse brain; antibody dilution 1:500.")
![Immunohistochemistry: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded U87 xenograft. Antibody at dilution of 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-NBP1-32505-img0009.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0010.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded RT2 xenograft; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0011.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-91020242014696.jpg)
![Immunocytochemistry/ Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-32505] - Amphiphysin/AMPH antibody [N1N2], N-term detects Amphiphysin/AMPH protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Amphiphysin/AMPH stained by Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-9102024204124.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-91020242001078.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser529] Antibody-Western Blot-NBP1-77808-img0005.jpg "Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.")
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0003.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 (NBP1-77808) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0004.jpg)
![Western Blot: HNF-3 alpha/FoxA1 Antibody (FOXA1/1512) [NBP2-53337] - Western Blot Analysis of human HeLa cell lysate using HNF-3 alpha/FoxA1 Antibody (FOXA1/1512).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-FOXA1-1512-Western-Blot-NBP2-53337-img0005.jpg "Western Blot: HNF-3 alpha/FoxA1 Antibody (FOXA1/1512) [NBP2-53337] - Western Blot Analysis of human HeLa cell lysate using HNF-3 alpha/FoxA1 Antibody (FOXA1/1512).")
![Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (FOXA1/1512) [NBP2-53337] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeled with HNF-3 alpha/FoxA1 Mouse Monoclonal Antibody (HNF-3 alpha/FoxA1/1512) followed by goat anti- mouse IgG-CF488. Counterstain is Phalloiden(Red).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-FOXA1-1512-Immunocytochemistry-Immunofluorescence-NBP2-53337-img0006.jpg)
![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1512) [NBP2-53337] - Formalin-fixed, paraffin-embedded human Prostate stained with FOXA1 Monoclonal Antibody (FOXA1/1512).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-FOXA1-1512-Immunohistochemistry-Paraffin-NBP2-53337-img0002.jpg)
![Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (FOXA1/1512) [NBP2-53337] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HNF-3 alpha/FoxA1 Mouse Monoclonal Antibody (HNF-3 alpha/FoxA1/1512) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-FOXA1-1512-Flow-Cytometry-NBP2-53337-img0007.jpg)
![Western Blot: HNF-3 alpha/FoxA1 Antibody (FOXA1/1512) [NBP2-53337] - Western Blot: HNF-3 alpha/FoxA1 Antibody (FOXA1/1512) [NBP2-54576] - (A) MCF-7 (B) PC3 Cell lysate Using FOXA1 Monoclonal Antibody (FOXA1/1512)](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-FOXA1-1512-Western-Blot-NBP2-53337-img0003.jpg)
![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1512) [NBP2-53337] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1512).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-FOXA1-1512-Immunohistochemistry-Paraffin-NBP2-53337-img0001.jpg)
![Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP2-53088-img0001.jpg "Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP2-53088] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Bim at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53088-img0003.jpg)
![Immunohistochemistry-Paraffin: Bim Antibody [NBP2-53088] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human endometrial cancer xenograft using Bim antibody at 1:50 dilution. The signal was detected using HRP labeled secondary antibody and DAB staining method which followed counter-staining with hematoxylin. The antibody generated a specific cytoplasmic staining with punctate appearance, signifying Bim/Bcl2L11 presence on mitochondria.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunohistochemistry-Paraffin-NBP2-53088-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0007.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)")
![Protein Array: S100B Antibody (S100B/1012) [NBP2-59618] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Protein-Array-NBP2-59618-img0001.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed. Paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Antibody (S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0006.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed, paraffin-embedded human Schwanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30877-img0008.jpg "Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Human cervical carcinoma. FOXA1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-Immunohistochemistry-Paraffin-NBP1-30877-img0007.jpg)
![Chromatin Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-30877-img0012.jpg)
![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Human breast carcinoma. FOXA1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-Immunohistochemistry-Paraffin-NBP1-30877-img0006.jpg)
![Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 ug whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 ug of FOXA1 antibody 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-Immunoprecipitation-NBP1-30877-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-30877_rabbit-polyclonal-hnf-3-alpha-foxa1-antibody-1610202419405736.jpg)
![Western Blot: NUT Antibody (46B3D2.1G6) [NBP2-31146] - WB analysis of NUT/ NUTM1 protein in (A) human testis lysate and on (B) partial recombinant NUT protein (expected molecular weight ~17kda) with NUT antibody (clone 46B3D2.1G6) at a concentration of 2 ug/ml. In human testis, this antibody detected Isoform 1 and Isoform 2 at ~120 kDa and ~46kDa respectively.](http://images.novusbio.com/fullsize/NUT-Antibody-46B3D2-1G6-Western-Blot-NBP2-31146-img0002.jpg "Western Blot: NUT Antibody (46B3D2.1G6) [NBP2-31146] - WB analysis of NUT/ NUTM1 protein in (A) human testis lysate and on (B) partial recombinant NUT protein (expected molecular weight ~17kda) with NUT antibody (clone 46B3D2.1G6) at a concentration of 2 ug/ml. In human testis, this antibody detected Isoform 1 and Isoform 2 at ~120 kDa and ~46kDa respectively.")
![Immunocytochemistry/Immunofluorescence: NUT Antibody (46B3D2.1G6) [NBP2-31146] - NUT antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.](http://images.novusbio.com/fullsize/NUT-Antibody-46B3D2-1G6-Immunocytochemistry-Immunofluorescence-NBP2-31146-img0006.jpg)
![Immunohistochemistry-Paraffin: NUT Antibody (46B3D2.1G6) [NBP2-31146] - IHC-P analysis of NUT protein in normal human skin tissue section using NUT antibody (clone 46B3D2.1G6) at a concentration of 5 ug/ml. This antibody stained the nuclei and cytoplasm of different epidermal cell types such as granulosa cells, keratinocytes, Langerhans' cell and pigmented cells, and dermal blood vessels.](http://images.novusbio.com/fullsize/NUT-Antibody-46B3D2-1G6-Immunohistochemistry-Paraffin-NBP2-31146-img0003.jpg)
![Western Blot: SMC3 Antibody [NBP2-20418] - Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NBP2-20418-img0003.jpg "Western Blot: SMC3 Antibody [NBP2-20418] - Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody [NBP2-20418] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20418-img0005.jpg)
![Immunohistochemistry-Paraffin: SMC3 Antibody [NBP2-20418] - Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunohistochemistry-Paraffin-NBP2-20418-img0004.jpg)
![Western Blot: SMC3 Antibody [NBP2-20418] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NBP2-20418-img0001.jpg)
![Western Blot: SMC3 Antibody [NBP2-20418] - Sample (30 ug of whole cell lysate) A: PC-12 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NBP2-20418-img0002.jpg)
![Immunoprecipitation: SMC3 Antibody [NBP2-20418] - Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 ug of SMC3 antibody. Western blot analysis was performed using SMC3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunoprecipitation-NBP2-20418-img0007.jpg)
![Western Blot: ch-TOG Antibody [NB500-182] - Specificity of polyclonal antibodies against ch-TOGp. Total cell protein (50 mg) from human HeLa cells was immunoblotted with a rabbit polyclonal serum generated against the C-terminal 301 amino acids of the ch-TOGp.](http://images.novusbio.com/fullsize/ch-TOG-Antibody-Western-Blot-NB500-182-img0002.jpg "Western Blot: ch-TOG Antibody [NB500-182] - Specificity of polyclonal antibodies against ch-TOGp. Total cell protein (50 mg) from human HeLa cells was immunoblotted with a rabbit polyclonal serum generated against the C-terminal 301 amino acids of the ch-TOGp.")
![Western Blot: S100A8/A9 Antibody (48M7C7) [NBP2-25256] - Analysis in A) recombinant protein, B) human spleen, C) HL60 and D) PC3 lysate using S1008A antibody at 0.5 ug/ml. Goat anti-Mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/S100A8-A9-Antibody-48M7C7-Western-Blot-NBP2-25256-img0001.jpg "Western Blot: S100A8/A9 Antibody (48M7C7) [NBP2-25256] - Analysis in A) recombinant protein, B) human spleen, C) HL60 and D) PC3 lysate using S1008A antibody at 0.5 ug/ml. Goat anti-Mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: S100A8/A9 Antibody (48M7C7) [NBP2-25256] - FFPE human spleen stained with S100A8 antibody (2 ug/ml), peroxidase-conjugate and DAB chromogen. TMA was used for this test.](http://images.novusbio.com/fullsize/S100A8-A9-Antibody-48M7C7-Immunohistochemistry-Paraffin-NBP2-25256-img0002.jpg)
![Flow Cytometry: S100A8/A9 Antibody (48M7C7) [NBP2-25256] - Analysis of PE conjugate of NBP2-25256. An intracellular stain was performed on SH-SY5Y cells with S100A8/A9 (48M7C7) antibody NBP2-25269PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.](http://images.novusbio.com/fullsize/S100A8-A9-Antibody-48M7C7-Flow-Cytometry-NBP2-25256-img0003.jpg)
![Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NB100-58833-img0002.jpg "Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: eIF4E Antibody [NB100-58833] - Detection of human eIF4E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4E antibody NB100-58833 used for IP at 3 ug per reaction. eIF4E was also immunoprecipitated by another rabbit anti-eIF4E antibody. For blotting immunoprecipitated eIF4E, NB100-58833 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunoprecipitation-NB100-58833-img0003.jpg)