| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36567SS | |||
| Azide and BSA Free | NBP2-80653 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33240 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33245 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33176 | |||
| IHC-Prediluted | NBP2-44945 | |||
![Immunoprecipitation: CD9 Antibody (IVA50) [NB500-494] - Fig. 1. Immunoprecipitation of human CD9 from the biotin-labeled human platelets lysates. Lane 1: immunoprecipitation with anti-CD9 (IVA50) Lane 2: immunoprecipitation with anti-human CD9 control antibody Lane 3: immunoprecipitation with negative control.](http://images.novusbio.com/fullsize/CD9-Antibody-IVA50-Immunoprecipitation-NB500-494-img0001.jpg "Immunoprecipitation: CD9 Antibody (IVA50) [NB500-494] - Fig. 1. Immunoprecipitation of human CD9 from the biotin-labeled human platelets lysates. Lane 1: immunoprecipitation with anti-CD9 (IVA50) Lane 2: immunoprecipitation with anti-human CD9 control antibody Lane 3: immunoprecipitation with negative control.")
![Western Blot: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of MMP13 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysate Lane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Western-Blot-NBP2-66954-img0009.jpg "Western Blot: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of MMP13 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysate Lane 2: MCF-7 cell lysate")
![Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954] - Staining MMP13 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunocytochemistry-Immunofluorescence-NBP2-66954-img0008.jpg)
![Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of paraffin-embedded human lung cancer tissue using anti-MMP13 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunohistochemistry-Paraffin-NBP2-66954-img0006.jpg)
![Flow Cytometry: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of Hela cells with MMP13 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Flow-Cytometry-NBP2-66954-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954] - Staining MMP13 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunocytochemistry-Immunofluorescence-NBP2-66954-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954] - Staining MMP13 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunocytochemistry-Immunofluorescence-NBP2-66954-img0003.jpg)
![Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP13 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunohistochemistry-Paraffin-NBP2-66954-img0004.jpg)
![Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of paraffin-embedded human breast tissue using anti-MMP13 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-13-Antibody-JF0893-Immunohistochemistry-Paraffin-NBP2-66954-img0005.jpg)
![Western Blot: CD63 Antibody (7G4.2E8) [NBP2-36567] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate.](http://images.novusbio.com/fullsize/CD63-Antibody-7G4-2E8-Western-Blot-NBP2-36567-img0002.jpg "Western Blot: CD63 Antibody (7G4.2E8) [NBP2-36567] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate.")
![Flow Cytometry: CD63 Antibody (7G4.2E8) [NBP2-36567] - Intracellular staining of mouse CD63 in Flow cytometry using 2.0 ug of antibody per 1 million cells. Isotype control was rat IgG2a lambda.](http://images.novusbio.com/fullsize/CD63-Antibody-7G4-2E8-Flow-Cytometry-NBP2-36567-img0003.jpg)
![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0005.jpg "Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16182-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - Human ovarian cancer, using DNA ligase IV antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP2-16182-img0002.jpg)
![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0001.jpg)
![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus and nucleolus by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-91020242041277.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-Western-Blot-NBP2-15975-img0015.jpg "Western Blot: COX4 Antibody [NBP2-15975] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: COX4 Antibody [NBP2-15975] - COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: COX4 stained by COX4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/COX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15975-img0014.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody [NBP2-15975] - COX4 antibody detects COX4 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. COX4 stained by COX4 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/COX4-Antibody-Immunohistochemistry-Paraffin-NBP2-15975-img0016.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Sample (50 ug of whole cell lysate) A: rat muscle, 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/COX-IV-isoform-1-Antibody-Western-Blot-NBP2-15975-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody [NBP2-15975] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-Western-Blot-NBP2-15975-img0006.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-Western-Blot-NBP2-15975-img0013.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (NBP2-15975) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15975_rabbit-polyclonal-cox4-antibody-9102024204120.jpg)
![Immunocytochemistry/ Immunofluorescence: COX4 Antibody [NBP2-15975] - COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: COX4 stained by COX4 antibody (NBP2-15975) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp2-15975_rabbit-polyclonal-cox4-antibody-91020242063363.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. CRISPR knockout cell lines generated by Edigene](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-30883] - Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. CRISPR knockout cell lines generated by Edigene")
![<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-30883] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (NBP1-30883). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-30883] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 protein stained by Stathmin 1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30883-img0007.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:H129912% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0003.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE Stathmin 1 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0009.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12 % SDS-PAGE Stathmin 1 antibody dilution: 1:2000](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-30883] - Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 protein stained by Stathmin 1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30883-img0006.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP1-30883] - Sample: Paraffin-embedded human breast carcinoma. Stathmin 1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30883-img0005.jpg)
![Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/RB1-Antibody-172C1094-cleaved-Knockdown-Validated-NB100-56598-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in HL60 cells were treated with camptothecin for 24 hours.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Western-Blot-NB100-56598-img0005.jpg)
![Immunohistochemistry: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, and The University of Chicago.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Immunohistochemistry-NB100-56598-img0004.jpg)
![Western Blot: MTA1 Antibody [NB600-260] - Detection of Human and Mouse MTA1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTA1 antibody NB600-260 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MTA1-Antibody-Western-Blot-NB600-260-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: MTA1 Antibody [NB600-260] - Detection of Human and Mouse MTA1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTA1 antibody NB600-260 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MTA1 Antibody [NB600-260] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- MTA1 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MTA1-Antibody-Immunohistochemistry-Paraffin-NB600-260-img0005.jpg)
![Immunoprecipitation: MTA1 Antibody [NB600-260] - Detection of human MTA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTA1 antibody NB600-260 (lot NB600-260-2) used for IP at 6 ug per reaction. MTA1 was also immunoprecipitated by rabbit anti-MTA1 antibody NB600-260-1. For blotting immunoprecipitated MTA1, NB600-260 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MTA1-Antibody-Immunoprecipitation-NB600-260-img0008.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NBP1-31258-img0009.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.")
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NBP1-31258-img0008.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-91020242001028.jpg)
![Immunocytochemistry/Immunofluorescence: GPER/GPR30 Antibody [NBP1-31258] - DIV11 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-16122024114732..png)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Mouse tissues.GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunohistochemistry-paraffin-1612202411499..png)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled AsPC-1 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518352213.jpg)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518344912.jpg)
![GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-1411202518434418.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) bolied and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518344921.jpg)
![GPER/GPR30 antibody detects GPER/GPR30 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPER/GPR30 stained by GPER/GPR30 antibody (NBP1-31258) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-141120251841523.jpg)
![Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis of Caspase 14 in mouse spleen lysate using Caspase 14 antibody at 1:1000. Novus's goat anti-mouse Ig HRP secondary antibody (Novus, 20101) and PicoTect ECL substrate solution (Novus, 10087K) were used for this test.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-70A1426-Western-Blot-NB100-56718-img0009.jpg "Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis of Caspase 14 in mouse spleen lysate using Caspase 14 antibody at 1:1000. Novus's goat anti-mouse Ig HRP secondary antibody (Novus, 20101) and PicoTect ECL substrate solution (Novus, 10087K) were used for this test.")
![Flow Cytometry: Caspase-14 Antibody (70A1426) [NB100-56718] - Intracellular analysis of Caspase-14 in 10^6 Jurkat cells probed with 1:500 dilution of Caspase-14 antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; red represents Caspase-14 antibody. This antibody was used for this test, and an anti-mouse IgG FITC conjugated secondary antibody.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-70A1426-Flow-Cytometry-NB100-56718-img0007.jpg)
![Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis of human breast cancer tissue using Caspase 14 antibody at a concentration of 0.1 ug/ml.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-70A1426-Western-Blot-NB100-56718-img0006.jpg)
![Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis for Caspase-14 using NB100-56718 at 2 ug/ml dilution against 10 ug of mouse spleen cell lysate.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-70A1426-Western-Blot-NB100-56718-img0008.jpg)
![Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (DA7).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Western-Blot-NBP2-29409-img0008.jpg "Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (DA7).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Confocal immunofluorescence image of MCF cells using Cytokeratin 18 Antibody (DA7). Green (CF488) and Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Immunocytochemistry-Immunofluorescence-NBP2-29409-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Formalin-paraffin human colon cancer stained with Cytokeratin 18 Ab (DA7). Note cytoplasmic staining of tumor cells.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Immunohistochemistry-Paraffin-NBP2-29409-img0003.jpg)
![Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Analysis of Cytokeratin 18 in HeLa lysate using Cytokeratin 18 (CK18) antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Western-Blot-NBP2-29409-img0002.jpg)
![Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Simple-Western-NBP2-29409-img0004.jpg)
![Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Simple-Western-NBP2-29409-img0005.jpg)
![Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis the level of TRIM25 and GRP78 in HCT116 and Huh7 cells treated with TM (5 ug/ml) or TG (1 uM) for 12 h. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP2-20710-img0006.jpg "Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis the level of TRIM25 and GRP78 in HCT116 and Huh7 cells treated with TM (5 ug/ml) or TG (1 uM) for 12 h. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP2-20710] - Human colon carcinoma, using TRIM25 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunohistochemistry-Paraffin-NBP2-20710-img0002.jpg)
![Western Blot: TRIM25 Antibody [NBP2-20710] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP2-20710-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis of UPR signaling, including phospho-JNK/JNK and phospho-eIF2alpha/eIF2alpha in HCT116 cells after stable knockdown of TRIM25 in HCT116 cells. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Knockdown-Validated-NBP2-20710-img0007.jpg)
![Immunohistochemistry: TRIM25 Antibody [NBP2-20710] - Knockdown of TRIM25 in HCC suppresses tumor growth in nude mice.a–c Huh7 cells stably expressing the indicated shRNAs (shNC, shTRIM25-1 & shTRIM25-2) were subcutaneously injected in nude mice respectively. Shown are average tumor volumes over time (n = 8) (a) & representative image (b) & weights (c) of tumors at day 25. d Kaplan–Meier analysis of overall survival percentage in nude mice inoculated with Huh7-shNC or Huh7-shTRIM25. The statistical significance was assessed using two-sided log-rank test according to mice with depletion of control or TRIM25. e, f Representative images (e) of IHC staining & the relative IHC scores (n = 8) (f) of TRIM25, Ki67, Keap1 & Nrf2 in HCC tissues of mice inoculated with Huh7-shNC or Huh7-shTRIM25-1&-2. Scale bar, 50 μm. g, h Representative IHC staining images (g) & statistical data (h) of TRIM25, Keap1 & Nrf2 expression in HCC tissues (TRIM25 low expression group, n = 45 & TRIM25 high expression group, n = 45). Scale bar, 50 μm. For a, c, f, & h, data represent the mean ± SEM. Statistical significance was assessed using two-tailed Student’s t-tests. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31953436), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-20710_rabbit-polyclonal-trim25-antibody-310202416121958.jpg)
