| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7258APC | |||
| Biotin | NB7258B | |||
| DyLight 350 | NB7258UV | |||
| DyLight 405 | NB7258V | |||
| DyLight 488 | NB7258G | |||
| DyLight 550 | NB7258R | |||
| DyLight 594 | NB7258DL594 | |||
| DyLight 650 | NB7258C | |||
| DyLight 680 | NB7258FR | |||
| DyLight 755 | NB7258IR | |||
| FITC | NB7259 | |||
| HRP | NB7260 | |||
| PE | NB7258PE | |||
| PerCP | NB7258PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7100APC | |||
| Biotin | NB7100B | |||
| DyLight 350 | NB7100UV | |||
| DyLight 405 | NB7100V | |||
| DyLight 488 | NB7100G | |||
| DyLight 550 | NB7100R | |||
| DyLight 594 | NB7100DL594 | |||
| DyLight 650 | NB7100C | |||
| DyLight 680 | NB7100FR | |||
| DyLight 755 | NB7100IR | |||
| FITC | NB7102 | |||
| HRP | NB7103 | |||
| PE | NB7100PE | |||
| PerCP | NB7100PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47911-0.1mg | |||
| IHC-Prediluted | NBP2-48363 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90051-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54473-100ug | |||
![Western Blot: CD19 Antibody [NBP2-15782] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CD19-Antibody-Western-Blot-NBP2-15782-img0016.jpg "Western Blot: CD19 Antibody [NBP2-15782] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CD19 Antibody [NBP2-15782] - Human tonsil. CD19 stained by CD19 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD19-Antibody-Immunohistochemistry-Paraffin-NBP2-15782-img0011.jpg)
![Flow Cytometry: CD19 Antibody [NBP2-15782] - Mouse splenocytes cell fixed in 4% paraformaldehyde at 4-C for 15 min. Black: Unlabelled sample was used as a control. Red: CD19 antibody [C1C3] dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/CD19-Antibody-Flow-Cytometry-NBP2-15782-img0006.jpg)
![Western Blot: CD19 Antibody [NBP2-15782] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/CD19-Antibody-Western-Blot-NBP2-15782-img0007.jpg)
![Immunohistochemistry-Paraffin: CD19 Antibody [NBP2-15782] - Human tonsil. Green: CD19 stained by CD19 antibody [C1C3] diluted at 1:250.Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD19-Antibody-Immunohistochemistry-Paraffin-NBP2-15782-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody [NBP2-15782] -Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD19 stained by CD19 antibody [C1C3] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-15782_rabbit-polyclonal-cd19-antibody-immunocytochemistry-immunofluorescence-35202413518..jpg)
![Flow Cytometry: Goat anti-Feline IgM Heavy Chain Secondary Antibody [NB7258] - The cat cells were washed and Incubated with the IgM antibody. It was used to detect Bcells. This image was submitted via customer review. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Feline-IgM-Secondary-Antibody-Flow-Cytometry-NB7258-img0001.jpg "Flow Cytometry: Goat anti-Feline IgM Heavy Chain Secondary Antibody [NB7258] - The cat cells were washed and Incubated with the IgM antibody. It was used to detect Bcells. This image was submitted via customer review. Image from the FITC version of this antibody.")
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)")
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - KG-1 cells using CD34 Monoclonal Antibody (HPCA1/1171) (red) & isotype control (green).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Flow-Cytometry-NBP2-44569-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0002.jpg)
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Percent of EPCs (CD133 + CD34 +) reduced in the XYK group detected by flow cytometry. Data are mean +/- SD analyzed by one-way analysis of variance. **P < 0.01. CSDH, chronic subdural hematoma; EPC, endothelial progenitor cell; XYK, Xiaoyukang Jiaonang. PE conjugated CD34 antibody used (NBP2-47911PE). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32328124/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-44569_mouse-monoclonal-cd34-antibody-hpca1-1171-177202495947.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Secondary Antibody [DyLight 488] [NBP1-72873] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB120-9167-img0001.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Secondary Antibody [DyLight 488] [NBP1-72873] Image from the DyLight 488 version of this antibody.")
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0002.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0007.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Analysis in human spleen and cerebral cortex tissues. Corresponding MS4A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90051-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Analysis in human spleen and cerebral cortex tissues. Corresponding MS4A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human cerebral cortex, lymph node, spleen and testis using Anti-MS4A1 antibody NBP1-90051 (A) shows similar protein distribution across tissues to independent antibody NBP1-90052 (B).](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90051-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CD20 Antibody [NBP1-90051] - Analysis using Anti-MS4A1 antibody NBP1-90051 (A) shows similar pattern to independent antibody NBP1-90052 (B).](http://images.novusbio.com/fullsize/CD20-Antibody-Western-Blot-NBP1-90051-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: CD20 Antibody [NBP1-90051] - Staining of human cell line REH shows localization to nucleus & plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90051-img0009.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human spleen using Anti-MS4A1 antibody NBP1-90051.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90051-img0021.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human testis.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90051-img0012.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human spleen shows strong membranous positivity in cells in white pulp.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90051-img0013.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human gastrointestinal shows strong membranous positivity in lymphocytes.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90051-img0014.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90051-img0015.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human tonsil shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90051-img0016.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90051-img0017.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human cerebral cortex using Anti-MS4A1 antibody NBP1-90051.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90051-img0019.jpg)
![Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human lymph node using Anti-MS4A1 antibody NBP1-90051.](http://images.novusbio.com/fullsize/CD20-Antibody-Immunohistochemistry-Paraffin-NBP1-90051-img0020.jpg)
![Immunohistochemistry-Paraffin: Rabbit Polyclonal CD20 Antibody [NBP1-90051] - CD20 immunoreactivity in an FFPE section of human tonsil. NBP1-90051 was diluted to 250ng per mL and was left on tissue sections for 30m at room temperature. Secondary was horse anti rabbit HRP polymer. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-90051_rabbit-polyclonal-cd20-antibody-immunohistochemistry-paraffin-1552024163744..png)
![Immunohistochemistry-Paraffin: Rabbit Polyclonal CD20 Antibody [NBP1-90051] - CD20 immunoreactivity in an FFPE section of mouse spleen. NBP1-90051 was diluted to 250ng per mL and was left on tissue sections for 30m at room temperature. Secondary was equine anti rabbit HRP polymer. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-90051_rabbit-polyclonal-cd20-antibody-immunohistochemistry-paraffin-285202412207..png)
![Immunohistochemistry-Paraffin: Rabbit Polyclonal CD20 Antibody [NBP1-90051] - CD20 immunoreactivity in an FFPE section of horse spleen. NBP1-90051 was diluted to 250ng per mL and was left on tissue sections for 30m at room temperature. Secondary was equine anti rabbit HRP polymer. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-90051_rabbit-polyclonal-cd20-antibody-immunohistochemistry-paraffin-285202412228..png)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0005.jpg "Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PE-CyTM7.")
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Staining of human peripheral blood with anti-human CD38 (HIT2) PE-CyTM5.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0006.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Analysis (surface staining) of human peripheral blood with anti-human CD38 (HIT2) Pacific Blue(TM).](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0012.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Analysis using the PE conjugate of NB500-510. Surface staining of PHA stimulated human peripheral blood lymphocytes with anti-human CD38 (HIT2) PE.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0002.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) Alexa Fluor(R) 700.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0003.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) FITC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0004.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0007.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PerCP.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0008.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) APC-CyTM7.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0009.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) APC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0010.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) Pacific Orange(TM).](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0011.jpg)
![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Western Blot Analysis of Raji cell lysate using Siglec-2/CD22 Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Western-Blot-NBP2-53239-img0008.jpg "Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Western Blot Analysis of Raji cell lysate using Siglec-2/CD22 Antibody (BLCAM/1795).")
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunocytochemistry-Immunofluorescence-NBP2-53239-img0005.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Formalin-fixed, paraffin-embedded human Spleen stained with Siglec-2/CD22 Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunohistochemistry-Paraffin-NBP2-53239-img0006.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Flow Cytometric Analysis of paraformaldehyde-fixed MOLT4 cells. Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Flow-Cytometry-NBP2-53239-img0004.jpg)
![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Analysis of Raji and Ramos Cell Lysates using CD22 Monoclonal Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Western-Blot-NBP2-53239-img0001.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD22 Monoclonal Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunohistochemistry-Paraffin-NBP2-53239-img0002.jpg)
![Protein Array: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Siglec-2/CD22 Antibody (BLCAM/1795) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Protein-Array-NBP2-53239-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: CD19 Antibody (JF100-06) [NBP2-67229] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD19 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunohistochemistry-Paraffin-NBP2-67229-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0002.jpg)
![Immunohistochemistry-Paraffin: CD19 Antibody (JF100-06) [NBP2-67229] - Analysis of paraffin-embedded human spleen tissue using anti-CD19 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunohistochemistry-Paraffin-NBP2-67229-img0003.jpg)
![Western Blot: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of CD19 on different lysates with Rabbit anti-CD19 antibody at 1/1,000 dilution.Lane 1: Raji cell lysateLane 2: Daudi cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 61 kDaObserved band size: 90 kDaExposure time: 2 minutes;8% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-western-blot-1382024114350..jpg)
![Flow Cytometry: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of Daudi cells labeling CD19.Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-flow-cytometry-138202411462..jpg)
![Flow Cytometry: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of Raji cells labeling CD19.Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-flow-cytometry-1382024114721..jpg)
![Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-western-blot-1311202411142..png "Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.")
![Flow Cytometry: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Antibody: Rabbit anti-CD86 recombinantmonoclonal [BLR030F] or isotype control(unshaded). Secondary: DyLight® 488-conjugated goatanti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-flow-cytometry-1311202411333..png)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -FFPE section of tonsil. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunohistochemistry-paraffin-131120241151..png)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -Section of HDLM-2 cells. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunocytochemistry-immunofluorescence-1311202411712..png)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: NBP2-76417 Lot 4 used for immunoprecipitation at 6 ul per reaction. B7-2/CD86 was also immunoprecipitated by a previous lot of this antibody (NBP2-76417 Lot 1) and a second antibody, NBP2-76416, against a different epitope of B7-2/CD86 (BLR029F). For blotting immunoprecipitated CD86, NBP2-76417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-1312025145029..jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417) and rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416).](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-131202515750..jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) biotin, streptavidin.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0012.jpg "Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) biotin, streptavidin.")
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PE.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0001.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) FITC.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0002.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood (lymphocyte gate) with anti-CD20 (2H7) PE-CyTM7.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0003.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood (lymphocyte gate) with anti-CD20 (2H7) PE-CyTM5.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0004.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) Alexa Fluor(R) 700.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0005.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) APC-CyTM7.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0006.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0007.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0008.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PE-DyLigt(R) 594.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0009.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PerCP.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0010.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) APC.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0011.jpg)
![Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP2-60672-img0011.jpg "Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher. Image using the Biotin form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc). Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60672-img0007.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0008.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0012.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Goat anti-Rat IgM Heavy Chain Secondary Antibody-Fluorophore-linked immunosorbent assay-NBP2-60672-img0014.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0013.jpg)