DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="BCL2 was detected in immersion fixed paraffin-embedded sections of human Non-Hodgkin’s Lymphoma using Mouse Anti-Human BCL2, Monoclonal Antibody (Catalog #NBP2-15200) at a 1:500 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). TTissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33315 | |||
| IHC-Prediluted | NBP2-44777 | |||
.png "BCL2 was detected in immersion fixed paraffin-embedded sections of human Non-Hodgkin’s Lymphoma using Mouse Anti-Human BCL2, Monoclonal Antibody (Catalog #NBP2-34443) at a 1:500 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). TTissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=")
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="BCL2 was detected in immersion fixed paraffin-embedded sections of human Non-Hodgkin’s Lymphoma using Mouse Anti-Human BCL2, Monoclonal Antibody (Catalog #NBP2-34443) at a 1:500 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). TTissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44780 | |||
| Azide and BSA Free | NBP2-34513-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24538SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34419-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-296SS | |||
| Azide and BSA Free | NBP2-80763 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37828-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47738-0.1mg | |||
| IHC-Prediluted | NBP2-48082 | |||
![Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (100/D5).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Western-Blot-NBP2-15200-img0006.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (100/D5) [NBP2-15200] - Formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 Ab (100/D5). Note nuclear membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Immunohistochemistry-Paraffin-NBP2-15200-img0005.jpg)
![Flow Cytometry: Bcl-2 Antibody (100/D5) [NBP2-15200] - Intracellular staining of Bcl-2 in fixed and permeabilized Jurkat cells using Bcl-2 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Flow-Cytometry-NBP2-15200-img0002.jpg)
![Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200] - analysis of Bcl-2 in human skin using Bcl-2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Western-Blot-NBP2-15200-img0004.jpg)
![Western Blot: c-Myc Antibody [NB600-334] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-c-myc antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-334-img0005.jpg "Western Blot: c-Myc Antibody [NB600-334] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-c-myc antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: Bcl-2 Antibody (124) [NBP2-34443] - Western Blot Analysis of MCF-7 cells using Bcl-2 Antibody (124).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-124-Western-Blot-NBP2-34443-img0005.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) [NBP2-34443] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Mouse Monoclonal Antibody (124).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-124-Immunohistochemistry-Paraffin-NBP2-34443-img0006.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) [NBP2-34443] - Formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with Bcl-2 MAb (124). Note nuclear membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-124-Immunohistochemistry-Paraffin-NBP2-34443-img0001.jpg)
![Western Blot: USP9x Antibody (1C4) [H00008239-M01] - USP9x in mouse ovarian cancer cell lysate using anti-USP9x antibody. The primary antibody was used at a dilution of 1:500 and incubated for overnight at 4C in 5% milk with PBST. Image from verified customer review.](http://images.novusbio.com/fullsize/USP9x-Antibody-1C4-Western-Blot-H00008239-M01-img0004.jpg "Western Blot: USP9x Antibody (1C4) [H00008239-M01] - USP9x in mouse ovarian cancer cell lysate using anti-USP9x antibody. The primary antibody was used at a dilution of 1:500 and incubated for overnight at 4C in 5% milk with PBST. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (1C4) [H00008239-M01] - Analysis of monoclonal antibody to USP9X on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/USP9x-Antibody-1C4-Immunocytochemistry-Immunofluorescence-H00008239-M01-img0006.jpg)
![Western Blot: USP9x Antibody (1C4) [H00008239-M01] - USP9X expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/USP9x-Antibody-1C4-Western-Blot-H00008239-M01-img0001.jpg)
![ELISA: USP9x Antibody (1C4) [H00008239-M01] - Detection limit for recombinant GST tagged USP9X is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-1C4-ELISA-H00008239-M01-img0008.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0027.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94877] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0028.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG antibody (NB110-53818) used.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0031.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0025.jpg)
![Simple Western: Autophagy Antibody Pack [NB910-94877] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Simple-Western-NB910-94877-img0037.jpg)
![Flow Cytometry: Autophagy Antibody Pack [NB910-94877] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Flow-Cytometry-NB910-94877-img0035.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0038.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0036.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0040.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody (NB110-60928) at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0041.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0017.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0032.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A (NB110-56893) in mouse intestine.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0018.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody [NBP2-24538] - Human prostate stained with TLR4 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-Paraffin-NBP2-24538-img0002.jpg)
![Western Blot: TLR4 Antibody [NBP2-24538] - Analysis of TLR4 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide using TLR4 antibody at 0.1 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP2-24538-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (SPM543) [NBP2-34372] - Immunofluorescence staining of HepG2 cells using TNF-alpha Mouse Monoclonal Antibody (SPM543) followed by goat anti-mouse IgG-CF488 (green). Counterstain is RedDot.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-SPM543-Immunocytochemistry-Immunofluorescence-NBP2-34372-img0003.jpg "Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (SPM543) [NBP2-34372] - Immunofluorescence staining of HepG2 cells using TNF-alpha Mouse Monoclonal Antibody (SPM543) followed by goat anti-mouse IgG-CF488 (green). Counterstain is RedDot.")
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0007.jpg "Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.")
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Ponceau S staining of HeLa whole cell lysate using NB800-PC1.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0003.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Analysis of STIM-1 antibody (NB110-60547) using HeLa whole cell lysate [NB800-PC1].](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0004.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of 30ug of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal Survivin antibody (NB 500-201) at 1ug/ml concentration. Detection was performed using ECL based method with 1 minute exposure time.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0005.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal NBS1 antibody (NB100-143) with detection employing ECL method.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0006.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Immunohistochemistry-Paraffin-NB100-296-img0001.jpg "Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.")
![Western Blot: HIF-1 alpha Antibody (HA111) [NB100-296] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Western-Blot-NB100-296-img0004.jpg)
![Western Blot: TFEB Antibody (S1) [H00007942-M01] - TFEB monoclonal antibody (M01), clone S1. Analysis of TFEB expression in rat brain.](http://images.novusbio.com/fullsize/TFEB-Antibody-S1-Western-Blot-H00007942-M01-img0009.jpg "Western Blot: TFEB Antibody (S1) [H00007942-M01] - TFEB monoclonal antibody (M01), clone S1. Analysis of TFEB expression in rat brain.")
![Immunocytochemistry/Immunofluorescence: TFEB Antibody (S1) [H00007942-M01] - Analysis of monoclonal antibody to TFEB on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/TFEB-Antibody-S1-Immunocytochemistry-Immunofluorescence-H00007942-M01-img0010.jpg)
![Western Blot: TFEB Antibody (S1) [H00007942-M01] - TFEB monoclonal antibody (M01), clone 3E1-G6 Western Blot analysis of TFEB expression in Jurkat ( Cat # L017V1 ).](http://images.novusbio.com/fullsize/TFEB-Antibody-S1-Western-Blot-H00007942-M01-img0011.jpg)
![ELISA: TFEB Antibody (S1) [H00007942-M01] - TFEB Antibody (3E1-G6) [H00007942-M01] - Detection limit for recombinant GST tagged TFEB is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TFEB-Antibody-S1-ELISA-H00007942-M01-img0008.jpg)
![Western Blot: TFEB Antibody (S1) [H00007942-M01] - GDF11 inhibits TFEB activity. (a) AML‐12 cells were cultured for 48 h in presence of GDF11 (100 ng/ml). qRT‐PCR analysis of the mRNA levels of TFEB & its target genes VPS11 & ATP6V1H. (b) AML‐12 cells transient expressing GFP‐TFEB were cultured for 48 h in presence of GDF11 (100 ng/ml). Immunofluorescence confocal microscopy showing TFEB localization with or without GDF11 (original magnification, 400×). (c) Nuclear TFEB fluorescence intensity normalized to vehicle controls. (d) Western blot analysis of total TFEB protein expression. (e) Densitometry analysis of the total TFEB. (f) Western blot analysis of nuclear TFEB protein expression. (g) Densitometry analysis of the nuclear TFEB. The experiment was performed in triplicate with similar results. The data are shown as mean ± SD, *p < 0.05 compared to the vehicle group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34905649), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007942-m01_mouse-monoclonal-tfeb-antibody-s1-310202415482264.jpg)
![Western Blot: TFEB Antibody (S1) [H00007942-M01] - TFEB expression reduces PHF1 levels in the P301S model of tauopathy, & significantly attenuates the loss of neuronal & synaptic markers in the hippocampus. A, Lysates were prepared from the hippocampal regions of WT, P301S, & P301S/flag-TFEB mice, & were subjected to immunoblotting. B, Quantitation by ImageJ revealed PHF1 levels of 436% in the P301S mice compared with WT controls, which were significantly attenuated to 212% in the P301S/flag-TFEB double-transgenic mice. However, TFEB expression did not alter the levels of total tau, which remained similar in the P301S & the double-transgenic mice. NeuN levels were reduced by 25% in the P301S mice, which was significantly reversed to WT levels in the double-transgenic mice. Similarly, spinophilin levels were reversed from 40% reduction to 6% due to TFEB overexpression. TFEB expression also increased LAMP1 levels by 207% & cathepsin D levels by 33% in the P301S/flag-TFEB mice compared with WT controls. Statistical analysis by one-way ANOVA followed by Student–Newman–Keuls post hoc test revealed significant differences. *p < 0.05, **p < 0.01, & ***p < 0.001, compared with WT controls; $$p < 0.01, $$$p < 0.001, compared with P301S mice. The data are reported as the mean ± SEM. n = 4 WT mice; n = 5 P301S & P301S/flag-TFEB mice. Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0042-16.2016), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007942-m01_mouse-monoclonal-tfeb-antibody-s1-310202415482238.jpg)
![Western Blot: TFEB Antibody (S1) [H00007942-M01] - GDF11 inhibits TFEB activity. (a) AML‐12 cells were cultured for 48 h in presence of GDF11 (100 ng/ml). qRT‐PCR analysis of the mRNA levels of TFEB & its target genes VPS11 & ATP6V1H. (b) AML‐12 cells transient expressing GFP‐TFEB were cultured for 48 h in presence of GDF11 (100 ng/ml). Immunofluorescence confocal microscopy showing TFEB localization with or without GDF11 (original magnification, 400×). (c) Nuclear TFEB fluorescence intensity normalized to vehicle controls. (d) Western blot analysis of total TFEB protein expression. (e) Densitometry analysis of the total TFEB. (f) Western blot analysis of nuclear TFEB protein expression. (g) Densitometry analysis of the nuclear TFEB. The experiment was performed in triplicate with similar results. The data are shown as mean ± SD, *p < 0.05 compared to the vehicle group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34905649), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007942-m01_mouse-monoclonal-tfeb-antibody-s1-310202415483630.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0019.jpg "Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Theoretical molecular weight: 36 kDa.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) in HeLa. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a GAPDH monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0012.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of paraffin-embedded human breast carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0015.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in Hela cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0008.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 5ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0018.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 20ug of THP-1 whole cell lysates per well onto an SDS-PAGE gel. GAPDH theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0011.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of mouse colon tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0013.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0014.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in NIH-3T3 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0006.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in MCF-7 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0007.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (9E10.3) [NBP2-45144] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (9E10.3).](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Immunohistochemistry-Paraffin-NBP2-45144-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (9E10.3) [NBP2-45144] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (9E10.3).")
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Simple Western lane view shows a specific band for c-Myc in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Simple-Western-NBP2-45144-img0002.jpg)
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Electropherogram image of the corresponding Simple Western lane. c-Myc antibody was used at 50 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Simple-Western-NBP2-45144-img0005.jpg)
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Lane view shows a specific band for c-Myc in 0.2 mg/mL of HeLa lysate and antibody at 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 74 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/nbp2-45144_mouse-monoclonal-c-myc-antibody-9e10-3-1772024959410.jpg)
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