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![Immunocytochemistry/ Immunofluorescence: PTEN [p Ser380] Antibody (JJ08-81) [NBP3-32791] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32791, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32791_rabbit-pten-p-ser380-mab-jj08-81-2920241192374.jpg "Immunocytochemistry/ Immunofluorescence: PTEN [p Ser380] Antibody (JJ08-81) [NBP3-32791] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32791, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Standard Reference Curve](http://images.novusbio.com/fullsize/Human-Mouse-Rat-VPS35-ELISA-Kit-Colorimetric-ELISA-NBP3-11045-img0001.jpg "ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Standard Reference Curve")
![ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - Linearity was determined by using low (rat kidney) and high (mouse kidney) natural samples, diluted 1/100 and mixed in various proportions.](http://images.novusbio.com/fullsize/Human-Mouse-Rat-VPS35-ELISA-Kit-Colorimetric-ELISA-NBP3-11045-img0002.jpg)
![ELISA: Human, Mouse, Rat VPS35 ELISA Kit (Colorimetric) [NBP3-11045] - VPS35 Determination of WT-A549 vs KN-549 Lysates Graph for the VPS35 Kit. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.](http://images.novusbio.com/fullsize/Human-Mouse-Rat-VPS35-ELISA-Kit-Colorimetric-ELISA-NBP3-11045-img0003.jpg)
![Western Blot: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Positive Western Blot detected in 293 whole cell lysate A549 whole cell lysate (treated with Calyculin A or EGF). All lanes: AMPK alpha 2 [p Thr172] Antibody at 1.95ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa Observed band size: 62 KDa](http://images.novusbio.com/fullsize/nbp3-26400_rabbit-ampk-alpha-2-p-thr-172-mab-4f4-282202411363013.jpg "Western Blot: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Positive Western Blot detected in 293 whole cell lysate A549 whole cell lysate (treated with Calyculin A or EGF). All lanes: AMPK alpha 2 [p Thr172] Antibody at 1.95ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa Observed band size: 62 KDa")
![Immunocytochemistry/Immunofluorescence: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Staining of A549 cells with AMPK alpha 2 [p Thr172] Antibody (4F4) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26400_rabbit-ampk-alpha-2-p-thr-172-mab-4f4-28220241323610.jpg)
![Western Blot: Rubicon Antibody [NBP1-30461] - Detection of Human Rubicon by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rubicon antibody NBP1-30461 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Rubicon-Antibody-Western-Blot-NBP1-30461-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rubicon Antibody [NBP1-30461] - Detection of Human Rubicon by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rubicon antibody NBP1-30461 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Rubicon Antibody [NBP1-30461] - Detection of human Rubicon by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rubicon antibody NBP1-30461 (lot NBP1-30461-2) used for IP at 6 ug per reaction. Rubicon was also immunoprecipitated by a previous lot of this antibody (lot NBP1-30461-1) and another rabbit anti-Rubicon antibody. For blotting immunoprecipitated Rubicon, NBP1-30461 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Rubicon-Antibody-Immunoprecipitation-NBP1-30461-img0004.jpg)