| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03522-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94753-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15492-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92493-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61853-0.025ml | |||
![Western Blot: BNIP3 Antibody (7Q7V3) [NBP3-15799] - Western blot analysis of extracts of various cell lines, using BNIP3 antibody (NBP3-15799) at 1:1000 dilution.Hela cells and NIH/3T3 cells were treated by Cobalt chloride (0.1 mM) at 37C for 4 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/BNIP3-Antibody-7Q7V3-Western-Blot-NBP3-15799-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: BNIP3 Antibody (7Q7V3) [NBP3-15799] - Western blot analysis of extracts of various cell lines, using BNIP3 antibody (NBP3-15799) at 1:1000 dilution.Hela cells and NIH/3T3 cells were treated by Cobalt chloride (0.1 mM) at 37C for 4 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BNIP3 Antibody (2P7B8) [BNIP3] - Immunohistochemistry analysis of paraffin-embedded Human liver using BNIP3 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15799_rabbit-bnip3-mab-2p7b8-52202517152742.jpg)
![Immunohistochemistry: BNIP3 Antibody (2P7B8) [BNIP3] - Immunohistochemistry analysis of paraffin-embedded Rat heart using BNIP3 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15799_rabbit-bnip3-mab-2p7b8-5220251726099.jpg)
![Immunohistochemistry: BNIP3 Antibody (2P7B8) [BNIP3] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using BNIP3 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15799_rabbit-bnip3-mab-2p7b8-52202517213599.jpg)
![Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Western-Blot-NBP3-03522-img0001.jpg "Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Rat heart using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516334192.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded human breast using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516293948.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-5220251628067.jpg)
![Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538] - Western blot analysis of extracts of various cell lines, using ATG4B Rabbit mAb (NBP3-16538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ATG4B-Antibody-9H6M6-Western-Blot-NBP3-16538-img0002.jpg "Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538] - Western blot analysis of extracts of various cell lines, using ATG4B Rabbit mAb (NBP3-16538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] - Immunofluorescence analysis of NIH-3T3 cells using ATG4B Rabbit mAb (NBP3-16538) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATG4B-Antibody-9H6M6-Immunocytochemistry-Immunofluorescence-NBP3-16538-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] - Confocal imaging of NIH/3T3 cells using ATG4B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16538_rabbit-atg4b-mab-9h6m6-7220251245390.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] - Confocal imaging of paraffin-embedded Mouse brain using ATG4B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . Objective: 40x. DAPI was used for nuclear staining (Blue). Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16538_rabbit-atg4b-mab-9h6m6-7220251245398.jpg)
![Western Blot: ULK1 Antibody - Azide and BSA Free [NBP2-94753] - Western blot analysis of various lysates, using ULK1 Rabbit pAb (A8529) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-94753_rabbit-polyclonal-ulk1-antibody-52202519302948.jpg "Western Blot: ULK1 Antibody - Azide and BSA Free [NBP2-94753] - Western blot analysis of various lysates, using ULK1 Rabbit pAb (A8529) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.")
![Western Blot: ULK1 Antibody [NBP2-94753] - Western blot analysis of extracts of Rat brain, using ULK1 antibody (NBP2-94753) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ULK1-Antibody-Western-Blot-NBP2-94753-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ULK1 Antibody - Azide and BSA Free [NBP2-94753] - Immunofluorescence analysis of NIH/3T3 cells using ULK1 Rabbit pAb (A8529) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94753_rabbit-polyclonal-ulk1-antibody-52202519302941.jpg)
![Immunocytochemistry/ Immunofluorescence: ULK1 Antibody - Azide and BSA Free [NBP2-94753] - Immunofluorescence analysis of HeLa cells using ULK1 Rabbit pAb (A8529) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94753_rabbit-polyclonal-ulk1-antibody-52202519313437.jpg)
![Western Blot: Huntingtin Antibody [NBP3-15492] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-15492-img0003.jpg "Western Blot: Huntingtin Antibody [NBP3-15492] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-15492] - Immunohistochemistry of paraffin-embedded rat brain using Huntingtin Rabbit pAb (NBP3-15492) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP3-15492-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody - Azide and BSA Free [Huntingtin] - Immunofluorescence analysis of U-251MG cells using Huntingtin Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15492_rabbit-huntingtin-pab-5220251734580.jpg)
![Western Blot: ATG10 Antibody [NBP2-92493] - Analysis of extracts of mouse small intestine, using ATG10 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/ATG10-Antibody-Western-Blot-NBP2-92493-img0001.jpg "Western Blot: ATG10 Antibody [NBP2-92493] - Analysis of extracts of mouse small intestine, using ATG10 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: ATG10 Antibody [NBP2-92493] - Immunohistochemistry of paraffin-embedded Rat testis using ATG10 Rabbit pAb (NBP2-92493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunohistochemistry-Paraffin-NBP2-92493-img0004.jpg)
![Immunohistochemistry-Paraffin: ATG10 Antibody [NBP2-92493] - Immunohistochemistry of paraffin-embedded Human colon using ATG10 Rabbit pAb (NBP2-92493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunohistochemistry-Paraffin-NBP2-92493-img0002.jpg)
![Immunohistochemistry-Paraffin: ATG10 Antibody [NBP2-92493] - Immunohistochemistry of paraffin-embedded Mouse spleen using ATG10 Rabbit pAb (NBP2-92493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunohistochemistry-Paraffin-NBP2-92493-img0003.jpg)
![Western Blot: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis using Beclin 1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-Western-Blot-NBP2-22176-img0004.jpg "Western Blot: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis using Beclin 1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.")
![Immunohistochemistry-Paraffin: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis of breast cancer tissues (left) and liver cancer tissues (right) using Beclin 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-Immunohistochemistry-Paraffin-NBP2-22176-img0002.jpg)
![Flow Cytometry: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis of RAJI cells using Beclin 1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-Flow-Cytometry-NBP2-22176-img0001.jpg)
![ELISA: Beclin 1 Antibody (2A4) [NBP2-22176] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-ELISA-NBP2-22176-img0003.jpg)
![Western Blot: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis using PRKAA2 mAb against human PRKAA2 (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-5B4G1-Western-Blot-NBP2-61853-img0004.jpg "Western Blot: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis using PRKAA2 mAb against human PRKAA2 (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)")
![Immunohistochemistry: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-5B4G1-Immunohistochemistry-NBP2-61853-img0003.jpg)
![Western Blot: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis using PRKAA2 mAb against HEK293 (1) and PRKAA2 (AA: 453-552)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-5B4G1-Western-Blot-NBP2-61853-img0005.jpg)
![Immunohistochemistry: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis of paraffin-embedded esophageal cancer tissues using PRKAA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-5B4G1-Immunohistochemistry-NBP2-61853-img0002.jpg)
![ELISA: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-5B4G1-ELISA-NBP2-61853-img0001.jpg)
![Western Blot: APE Antibody (12H1-R) [NBP3-32042] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32042) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32042) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32042_mouse-ape-mab-12h1-r-278202415242630.jpg "Western Blot: APE Antibody (12H1-R) [NBP3-32042] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32042) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32042) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: APE Antibody (12H1-R) [NBP3-32042] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-APE antibody (NBP3-32042) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32042) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32042_mouse-ape-mab-12h1-r-298202414333662.jpg)
![Immunocytochemistry/ Immunofluorescence: APE Antibody (12H1-R) [NBP3-32042] - Immunocytochemistry analysis of A549 cells labeling APE with Mouse anti-APE antibody (NBP3-32042) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-APE antibody (NBP3-32042) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32042_mouse-ape-mab-12h1-r-2920241114842.jpg)
![Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32783) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32783_rabbit-p70-s6-kinase-s6k-p-thr389-mab-psh02-23-29820241492679.jpg "Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32783) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunocytochemistry analysis of MCF7 cells treated with or without 20% FBS overnight then add 100nM Calyculin A for 30 minutes labeling p70 S6 Kinase/S6K with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32783_rabbit-p70-s6-kinase-s6k-p-thr389-mab-psh02-23-292024111483.jpg)
![Western Blot: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Western blot analysis of p70 S6 Kinase/S6K on different lysates with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 59 kDa Observed band size: 70/85 kDa Exposure time: Lane 1-4 (left): 1 minute 2 seconds; Lane 1-4 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32783_rabbit-p70-s6-kinase-s6k-p-thr389-mab-psh02-23-3920248453540.jpg)
![Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-288202412461629.jpg "Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTEN Antibody (JE51-96) [NBP3-32873] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32873) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32873) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29820241492644.jpg)
![Flow Cytometry: PTEN Antibody (JE51-96) [NBP3-32873] - Flow cytometric analysis of A431 cells labeling PTEN. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32873, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29202416372137.jpg)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)