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![Western Blot: ROBO1 Knockout A549 Cell Lysate [NBP3-18631] - Lane 1: Opal Prestained MW Marker. Lane 2: A549 ROBO KO Clone 5. Lane 3: A549 ROBO KO Clone 15. Lane 4: A549 ROBO KO Clone 17. Lane 5: A549 ROBO KO Clone 18. Lane 6: A549 ROBO KO Clone 21. Lane 7: A549 ROBO1 KO Bulk. Lane 8: A549 WCL Parental. Lane 9: MCF-7 WCL. Load: 35ug lysate/lane. Primary Antibody [Blot A] Anti-ROBO1 ~181kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30min at RT. Block: TTBS/Casein at RT. No detection of expected band at ~181kDa is observed in ROBO1 knockout A549 when compared with unmodified A549 cell lysates by Western blot.](http://images.novusbio.com/fullsize/ROBO1-Knockout-A549-Cell-Lysate-Western-Blot-NBP3-18631-img0001.jpg "Western Blot: ROBO1 Knockout A549 Cell Lysate [NBP3-18631] - Lane 1: Opal Prestained MW Marker. Lane 2: A549 ROBO KO Clone 5. Lane 3: A549 ROBO KO Clone 15. Lane 4: A549 ROBO KO Clone 17. Lane 5: A549 ROBO KO Clone 18. Lane 6: A549 ROBO KO Clone 21. Lane 7: A549 ROBO1 KO Bulk. Lane 8: A549 WCL Parental. Lane 9: MCF-7 WCL. Load: 35ug lysate/lane. Primary Antibody [Blot A] Anti-ROBO1 ~181kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30min at RT. Block: TTBS/Casein at RT. No detection of expected band at ~181kDa is observed in ROBO1 knockout A549 when compared with unmodified A549 cell lysates by Western blot.")
![Western Blot: CTGF/CCN2 Antibody (GT28711) [NBP3-13686] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF/CCN2 antibody [GT28711] (NBP3-13686) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-GT28711-Western-Blot-NBP3-13686-img0001.jpg "Western Blot: CTGF/CCN2 Antibody (GT28711) [NBP3-13686] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF/CCN2 antibody [GT28711] (NBP3-13686) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody (GT28711) [NBP3-13686] - CTGF/CCN2 antibody [28711] detects CTGF/CCN2 protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: CTGF/CCN2 stained by CTGF/CCN2 antibody [28711] (NBP3-13686) diluted at 1:500.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-GT28711-Immunocytochemistry-Immunofluorescence-NBP3-13686-img0003.jpg)
![Western Blot: Erythropoietin/EPO Antibody (HL1793) - Azide and BSA Free [NBP3-25454] - Recombinant human EPO protein (CHO cells expressed) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1793] (NBP3-25454) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25454_rabbit-erythropoietin-epo-mab-hl1793-azide-and-bsa-free-2120249413378.jpg "Western Blot: Erythropoietin/EPO Antibody (HL1793) - Azide and BSA Free [NBP3-25454] - Recombinant human EPO protein (CHO cells expressed) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1793] (NBP3-25454) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Erythropoietin/EPO Antibody (HL1793) - Azide and BSA Free [NBP3-25454] - Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1794] (NBP3-25455) (5 ug/mL) Detection: HRP-conjugated EPO antibody [HL1793] (NBP3-25454) (1 ug/mL) Please notice that NBP3-25454 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25455. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25454_rabbit-erythropoietin-epo-mab-hl1793-azide-and-bsa-free-212024941429.jpg)
![ELISA: Erythropoietin/EPO Antibody (HL1793) - Azide and BSA Free [NBP3-25454] - Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1793] (NBP3-25454) (5 ug/mL) Detection: HRP-conjugated EPO antibody [HL1794] (NBP3-25455) (1 ug/mL) Please notice that NBP3-25455 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25454. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25454_rabbit-erythropoietin-epo-mab-hl1793-azide-and-bsa-free-2120249403886.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB4/Her4 Antibody (HFR-1) [NBP3-07160] - Immunofluorescence Analysis of MCF-7 cells labeling HER-4 with ErbB4/Her4 Mouse Monoclonal Antibody (HFR-1) Goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).](http://images.novusbio.com/fullsize/ErbB4-Her4-Antibody-HFR-1-Immunocytochemistry-Immunofluorescence-NBP3-07160-img0002.jpg "Immunocytochemistry/Immunofluorescence: ErbB4/Her4 Antibody (HFR-1) [NBP3-07160] - Immunofluorescence Analysis of MCF-7 cells labeling HER-4 with ErbB4/Her4 Mouse Monoclonal Antibody (HFR-1) Goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).")
![Flow Cytometry: ErbB4/Her4 Antibody (HFR-1) [NBP3-07160] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using ErbB4/Her4 Mouse Monoclonal Antibody (HFR-1) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/ErbB4-Her4-Antibody-HFR-1-Flow-Cytometry-NBP3-07160-img0001.jpg)
![Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2488R) [NBP3-07724] - Formalin-fixed, paraffin-embedded human Placenta stained with TIMP-2 Rabbit Recombinant Monoclonal Antibody (TIMP-2/2488R).](http://images.novusbio.com/fullsize/TIMP-2-Antibody-TIMP2-2488R-Immunohistochemistry-Paraffin-NBP3-07724-img0001.jpg "Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2488R) [NBP3-07724] - Formalin-fixed, paraffin-embedded human Placenta stained with TIMP-2 Rabbit Recombinant Monoclonal Antibody (TIMP-2/2488R).")
![Western Blot: Fibrinogen gamma chain Antibody (HL1395) - Azide and BSA Free [NBP3-25467] - Human plasma (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody [HL1395] (NBP3-25467) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25467_rabbit-fibrinogen-gamma-chain-mab-hl1395-azide-bsa-free-2120249425419.jpg "Western Blot: Fibrinogen gamma chain Antibody (HL1395) - Azide and BSA Free [NBP3-25467] - Human plasma (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody [HL1395] (NBP3-25467) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Fibrinogen gamma chain Antibody (HL1395) - Azide and BSA Free [NBP3-25467] - Fibrinogen gamma antibody [HL1395] detects Fibrinogen gamma protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrinogen gamma stained by Fibrinogen gamma antibody [HL1395] (NBP3-25467) diluted at 1:50. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25467_rabbit-fibrinogen-gamma-chain-mab-hl1395-azide-bsa-free-212024940383.jpg)