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![Western Blot: beta-Actin Antibody (RM112)[NBP3-18847]Western blot of A431 cells, using anti-beta -Actin RevMAb Clone RM112 at a 1/1000 dilution, showed a band of beta -Actin at the predicted MW (42 kDa).](http://images.novusbio.com/fullsize/nbp3-18847_rabbit-beta-actin-mab-rm112-161120231301818.png "Western Blot: beta-Actin Antibody (RM112)[NBP3-18847]Western blot of A431 cells, using anti-beta -Actin RevMAb Clone RM112 at a 1/1000 dilution, showed a band of beta -Actin at the predicted MW (42 kDa).")
![Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (RM112) [NBP3-18847] - Immunocytochemical staining of HeLa cells, using Clone RM112 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-18847_rabbit-beta-actin-mab-rm112-immunocytochemistry-immunofluorescence-189202523474818.png)
![Immunohistochemistry-Paraffin: beta-Actin Antibody (RM112) [NBP3-18847] - Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue using Clone RM112 at a 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-18847_rabbit-beta-actin-mab-rm112-immunohistochemistry-paraffin-18920252348536.png)
![Immunohistochemistry-Paraffin: RhoB Antibody [NBP2-20154] - detects RHOB protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft.](http://images.novusbio.com/fullsize/RhoB-Antibody-Immunohistochemistry-Paraffin-NBP2-20154-img0004.jpg "Immunohistochemistry-Paraffin: RhoB Antibody [NBP2-20154] - detects RHOB protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft.")
![Western Blot: RhoB Antibody [NBP2-20154] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/RhoB-Antibody-Western-Blot-NBP2-20154-img0001.jpg)
![Western Blot: RhoB Antibody [NBP2-20154] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RhoB-Antibody-Western-Blot-NBP2-20154-img0002.jpg)
![Western Blot: CTGF/CCN2 Antibody [NBP2-16026] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Western-Blot-NBP2-16026-img0007.jpg "Western Blot: CTGF/CCN2 Antibody [NBP2-16026] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CTGF Antibody [NBP2-16026] - Sample (30 ug of whole cell lysate) A: HUVEC 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CTGF-Antibody-Western-Blot-NBP2-16026-img0001.jpg)
![Block/Neutralize: Recombinant Human HIF-1 alpha Protein [NBC1-18422] - Images of normal mouse kidney showing (A) staining with HIF-1 alpha antibody NB100-479 and (B) staining blocking with NBC1-18422 used at 10-fold excess concentration vs HIF-1 alpha antibody. Images provided by Dr Yves Heremans and please see the product review for additional details.](http://images.novusbio.com/fullsize/Recombinant-Human-HIF-1-alpha-Protein-Antibody-Competition-NBC1-18422-img0007.jpg "Block/Neutralize: Recombinant Human HIF-1 alpha Protein [NBC1-18422] - Images of normal mouse kidney showing (A) staining with HIF-1 alpha antibody NB100-479 and (B) staining blocking with NBC1-18422 used at 10-fold excess concentration vs HIF-1 alpha antibody. Images provided by Dr Yves Heremans and please see the product review for additional details.")
![SDS-Page: Recombinant Human HIF-1 alpha Protein [NBC1-18422] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Human-HIF-1-alpha-Protein-SDS-Page-NBC1-18422-img0009.jpg)
![Western Blot: PYK2/FAK2 Antibody (GT858) [NBP3-13592] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody [GT858] (NBP3-13592) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-GT858-Western-Blot-NBP3-13592-img0001.jpg "Western Blot: PYK2/FAK2 Antibody (GT858) [NBP3-13592] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody [GT858] (NBP3-13592) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (GT858) [NBP3-13592] - PYK2/FAK2 antibody [GT858] detects PYK2/FAK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PYK2/FAK2 stained by PYK2/FAK2 antibody [GT858] (NBP3-13592) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-GT858-Immunohistochemistry-Paraffin-NBP3-13592-img0004.jpg)
![Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (GT858) [NBP3-13592] - PYK2/FAK2 antibody [GT858] detects PYK2/FAK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PYK2/FAK2 stained by PYK2/FAK2 antibody [GT858] (NBP3-13592) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-GT858-Immunohistochemistry-Paraffin-NBP3-13592-img0002.jpg)
![Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (GT858) [NBP3-13592] - PYK2/FAK2 antibody [GT858] detects PYK2/FAK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat thymus gland. PYK2/FAK2 stained by PYK2/FAK2 antibody [GT858] (NBP3-13592) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-GT858-Immunohistochemistry-Paraffin-NBP3-13592-img0003.jpg)
![Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FGFR1-[p-Tyr653--p-Tyr654]-Antibody-Western-Blot-NBP3-13251-img0003.jpg "Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Untreated (-) and treated (+) U87-MG whole cell extracts (40 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FGFR1-[p-Tyr653--p-Tyr654]-Antibody-Western-Blot-NBP3-13251-img0001.jpg)
![Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FGFR1-[p-Tyr653--p-Tyr654]-Antibody-Western-Blot-NBP3-13251-img0002.jpg)
![Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-p-Tyr576-Antibody-HL127-Western-Blot-NBP3-13675-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-p-Tyr576-Antibody-HL127-Western-Blot-NBP3-13675-img0002.jpg)
![Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody [HL127] (NBP3-13675) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13675_rabbit-fak-p-tyr-576-mab-hl127-910202420131253.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13675_rabbit-fak-p-tyr-576-mab-hl127-1610202419405780.jpg)
![Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13675_rabbit-fak-p-tyr-576-mab-hl127-western-blot-2611202519361.jpg)
![Western Blot: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa FGF-13 (pan) protein using Mouse Anti-FGF-13 (pan) Monoclonal Antibody, Clone S235-22 (NBP2-42178). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FGF-13 (pan) Monoclonal Antibody (NBP2-42178) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.](http://images.novusbio.com/fullsize/FGF-13-Antibody-S235-22-Pan-Specific-Western-Blot-NBP2-42178-img0003.jpg "Western Blot: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa FGF-13 (pan) protein using Mouse Anti-FGF-13 (pan) Monoclonal Antibody, Clone S235-22 (NBP2-42178). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FGF-13 (pan) Monoclonal Antibody (NBP2-42178) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.")
![Immunocytochemistry/Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody, Clone S235-22 (NBP2-42178). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody (NBP2-42178) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) FGFA/FHFA (pan) Antibody (D) Composite.](http://images.novusbio.com/fullsize/FGF-13-Antibody-S235-22-Pan-Specific-Immunocytochemistry-Immunofluorescence-NBP2-42178-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cell Projection, Nucleus, Cytoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/FGF-13-Antibody-S235-22-Pan-Specific-Immunocytochemistry-Immunofluorescence-NBP2-42178-img0002.jpg)
![Western Blot: Beta Ig-h3/TGFBI Antibody [NBP1-60049] - Titration: 1.25ug/ml Positive Control: Jurkat cell lysate.](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Western-Blot-NBP1-60049-img0001.jpg "Western Blot: Beta Ig-h3/TGFBI Antibody [NBP1-60049] - Titration: 1.25ug/ml Positive Control: Jurkat cell lysate.")
![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-60049] - human corneal tissue (epithelium and stroma) Primary 1:400 Secondary: Fluorescein Isothiocyanate-conjugated anti-rabbit IgG 1:350](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-60049-img0003.jpg)
![Western Blot: Beta Ig-h3/TGFBI Antibody [NBP1-60049] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%](http://images.novusbio.com/fullsize/Beta-Ig-h3-TGFBI-Antibody-Western-Blot-NBP1-60049-img0004.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0004.jpg "Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0001.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0002.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0003.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0005.jpg)