| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60506-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42178-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37270-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99548-50ul | |||
![Western Blot: ERK2 Antibody (4C11) [NB110-60506] - Analysis using ERK2 mouse mAb against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.](http://images.novusbio.com/fullsize/ERK2-Antibody-4C11-Western-Blot-NB110-60506-img0010.jpg "Western Blot: ERK2 Antibody (4C11) [NB110-60506] - Analysis using ERK2 mouse mAb against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (4C11) [NB110-60506] - Analysis of Eca-109 cells using ERK2 mouse mAb (green).](http://images.novusbio.com/fullsize/ERK2-Antibody-4C11-Immunocytochemistry-Immunofluorescence-NB110-60506-img0007.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (4C11) [NB110-60506] - Analysis of human lung carcinoma (left) and breast carcinoma (right) showing cytoplasmic localization using ERK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK2-Antibody-4C11-Immunohistochemistry-Paraffin-NB110-60506-img0009.jpg)
![Western Blot: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis using HAS1 mAb against human HAS1 (AA: 74-243) recombinant protein. (Expected MW is 44.4 kDa)](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-3E10-Western-Blot-NBP1-51635-img0007.jpg "Western Blot: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis using HAS1 mAb against human HAS1 (AA: 74-243) recombinant protein. (Expected MW is 44.4 kDa)")
![Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis of U251 cells using HAS1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-3E10-Immunocytochemistry-Immunofluorescence-NBP1-51635-img0005.jpg)
![Flow Cytometry: Aminopeptidase N/CD13 Antibody (22A5) [NBP1-28429] - Analysis using the FITC conjugate of NBP1-28429. Staining of granulocytes.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-22A5-Flow-Cytometry-NBP1-28429-img0002.jpg "Flow Cytometry: Aminopeptidase N/CD13 Antibody (22A5) [NBP1-28429] - Analysis using the FITC conjugate of NBP1-28429. Staining of granulocytes.")
![CyTOF-reported: Aminopeptidase N/CD13 Antibody (22A5) [NBP1-28429] - Human bone marrow underwent RBC lysis and surface staining with Gd-160 conjugate of CD13 (22A5) antibody at 2 ug/mL, followed by gating on standard surface markers. Neutrophils demonstrate characteristic CD13 loss and reacquisition, with partial reactivity on monocytes. Specificity is very good, with minimal reactivity on lymphocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-22A5-CyTOF-reported-NBP1-28429-img0004.jpg)
![Western Blot: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa FGF-13 (pan) protein using Mouse Anti-FGF-13 (pan) Monoclonal Antibody, Clone S235-22 (NBP2-42178). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FGF-13 (pan) Monoclonal Antibody (NBP2-42178) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.](http://images.novusbio.com/fullsize/FGF-13-Antibody-S235-22-Pan-Specific-Western-Blot-NBP2-42178-img0003.jpg "Western Blot: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa FGF-13 (pan) protein using Mouse Anti-FGF-13 (pan) Monoclonal Antibody, Clone S235-22 (NBP2-42178). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FGF-13 (pan) Monoclonal Antibody (NBP2-42178) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.")
![Immunocytochemistry/Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody, Clone S235-22 (NBP2-42178). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody (NBP2-42178) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) FGFA/FHFA (pan) Antibody (D) Composite.](http://images.novusbio.com/fullsize/FGF-13-Antibody-S235-22-Pan-Specific-Immunocytochemistry-Immunofluorescence-NBP2-42178-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cell Projection, Nucleus, Cytoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/FGF-13-Antibody-S235-22-Pan-Specific-Immunocytochemistry-Immunofluorescence-NBP2-42178-img0002.jpg)
![Western Blot: Tie-1 Antibody (8D12B10) [NBP2-37270] - Western blot analysis using TIE1 mouse mAb against HepG2 cell lysate.](http://images.novusbio.com/fullsize/Tie-1-Antibody-8D12B10-Western-Blot-NBP2-37270-img0004.jpg "Western Blot: Tie-1 Antibody (8D12B10) [NBP2-37270] - Western blot analysis using TIE1 mouse mAb against HepG2 cell lysate.")
![Immunohistochemistry: Tie-1 Antibody (8D12B10) [NBP2-37270] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TIE1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Tie-1-Antibody-8D12B10-Immunohistochemistry-NBP2-37270-img0005.jpg)
![Western Blot: Tie-1 Antibody (8D12B10) [NBP2-37270] - Western blot analysis using TIE1 mAb against human TIE1 (AA: 385-607) recombinant protein. (Expected MW is 50.6 kDa)](http://images.novusbio.com/fullsize/Tie-1-Antibody-8D12B10-Western-Blot-NBP2-37270-img0002.jpg)
![Western Blot: Tie-1 Antibody (8D12B10) [NBP2-37270] - Western blot analysis using TIE1 mAb against HEK293 (1) and TIE1 (AA: 385-607)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Tie-1-Antibody-8D12B10-Western-Blot-NBP2-37270-img0003.jpg)
![ELISA: Tie-1 Antibody (8D12B10) [NBP2-37270] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Tie-1-Antibody-8D12B10-ELISA-NBP2-37270-img0001.jpg)
![Western Blot: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163.](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Western-Blot-NBP2-71420-img0002.jpg "Western Blot: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163.")
![Immunocytochemistry/Immunofluorescence: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD163.](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71420-img0004.jpg)
![Immunohistochemistry: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71420-img0003.jpg)
![Western Blot: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of extracts (35ug) from 9 different cell lines by using CD163 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Western-Blot-NBP2-71420-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0005.jpg "Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in rat small intestine with rabbit polyclonal antibody at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in human brain with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0001.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in human small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0002.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in mouse brain with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0003.jpg)
![Immunohistochemistry-Paraffin: FGF-9 Antibody [NBP2-99548] - Staining FGF-9 in mouse small intestine with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/FGF-9-Antibody-Immunohistochemistry-Paraffin-NBP2-99548-img0004.jpg)
![Immunohistochemistry-Paraffin: Endostatin Antibody (1837.46) [NBP1-40051] - Analysis of anti-COL18A1 / Endostatin antibody with human kidney at dilution 1:100.](http://images.novusbio.com/fullsize/Endostatin-Antibody-1837-46-Immunohistochemistry-Paraffin-NBP1-40051-img0001.jpg "Immunohistochemistry-Paraffin: Endostatin Antibody (1837.46) [NBP1-40051] - Analysis of anti-COL18A1 / Endostatin antibody with human kidney at dilution 1:100.")
![Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Striated muscle from human tongue immunostained with 3B3 (1250).](http://images.novusbio.com/fullsize/Actin-Alpha-1-Skeletal-Muscle-Antibody-3B3-Immunohistochemistry-Paraffin-NBP1-97723-img0005.jpg "Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Striated muscle from human tongue immunostained with 3B3 (1250).")
![Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Paraffin sections of human striated skeletal muscle.](http://images.novusbio.com/fullsize/Actin-Alpha-1-Skeletal-Muscle-Antibody-3B3-Immunohistochemistry-Paraffin-NBP1-97723-img0003.jpg)
![Western Blot: pan Actin Antibody (A2E1-R) [NBP3-32712] - Western blot analysis of pan Actin on different lysates with Mouse anti-pan Actin antibody (NBP3-32712) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Mouse smooth muscle tissue lysate (40 ug/Lane) Lane 7: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32712) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-2782024163166.jpg "Western Blot: pan Actin Antibody (A2E1-R) [NBP3-32712] - Western blot analysis of pan Actin on different lysates with Mouse anti-pan Actin antibody (NBP3-32712) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Mouse smooth muscle tissue lysate (40 ug/Lane) Lane 7: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32712) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: pan Actin Antibody (A2E1-R) [NBP3-32712] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Mouse anti-pan Actin antibody (NBP3-32712) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32712) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-29820241492695.jpg)
![Immunocytochemistry/ Immunofluorescence: pan Actin Antibody (A2E1-R) [NBP3-32712] - Immunocytochemistry analysis of HeLa cells labeling pan Actin with Mouse anti-pan Actin antibody (NBP3-32712) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-pan Actin antibody (NBP3-32712) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-29202410334034.jpg)