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![Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody (4C6/4) [NBP3-07234] - Formalin-fixed, paraffin-embedded human Brain stained with p14ARF/CDKN2A Mouse Monoclonal Antibody (4C6/4).](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-4C6-4-Immunohistochemistry-Paraffin-NBP3-07234-img0001.jpg "Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody (4C6/4) [NBP3-07234] - Formalin-fixed, paraffin-embedded human Brain stained with p14ARF/CDKN2A Mouse Monoclonal Antibody (4C6/4).")
![Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody (4C6/4) [NBP3-07234] - Formalin-fixed, paraffin-embedded rat Brain stained with p14ARF/CDKN2A Mouse Monoclonal Antibody (4C6/4).](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-4C6-4-Immunohistochemistry-Paraffin-NBP3-07234-img0002.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Analysis in human cerebral cortex and pancreas tissues. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Analysis in human cerebral cortex and pancreas tissues. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human cerebellum, cerebral cortex, pancreas and testis using Anti-VGLUT1 antibody NBP2-33597 (A) shows similar protein distribution across tissues to independent antibody NBP2-39000 (B).](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0009.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0011.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0006.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0008.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-33597-img0010.jpg)
![Western Blot: MRP2 Antibody [NBP1-69023] - Sample Tissue: Mouse Thymus Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/MRP2-Antibody-Western-Blot-NBP1-69023-img0004.jpg "Western Blot: MRP2 Antibody [NBP1-69023] - Sample Tissue: Mouse Thymus Antibody Dilution: 1.0ug/ml")
![Western Blot: MRP2 Antibody [NBP1-69023] - Mouse Spleen lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/MRP2-Antibody-Western-Blot-NBP1-69023-img0003.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Sample Tissue: Human A549 Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-57622-img0007.jpg "Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Sample Tissue: Human A549 Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry: NKCC2/SLC12A1 Antibody [NBP1-57622] - Paraffin Embedded Tissue: Human Kidney Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-NBP1-57622-img0006.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Jurkat cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-57622-img0002.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody [NBP1-57622] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Western-Blot-NBP1-57622-img0005.jpg)
![Western Blot: VGLUT1 Antibody [NB100-1837] - Cell lysate from rat](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Western-Blot-NB100-1837-img0001.jpg "Western Blot: VGLUT1 Antibody [NB100-1837] - Cell lysate from rat")
![Western Blot: ABCA2 Antibody [NBP2-92339] - Analysis of extracts of various cell lines, using ABCA2 .Exposure time: 3min.](http://images.novusbio.com/fullsize/ABCA2-Antibody-Western-Blot-NBP2-92339-img0003.jpg "Western Blot: ABCA2 Antibody [NBP2-92339] - Analysis of extracts of various cell lines, using ABCA2 .Exposure time: 3min.")
![Immunocytochemistry/ Immunofluorescence: ABCA2 Antibody - Azide and BSA Free [NBP2-92339] - Immunofluorescence analysis of paraffin-embedded mouse brain using ABCA2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92339_rabbit-polyclonal-abca2-antibody-4220251429023.jpg%3Ft=1709177288)
![Immunocytochemistry/ Immunofluorescence: ABCA2 Antibody - Azide and BSA Free [NBP2-92339] - Immunofluorescence analysis of U-251 MG cells using ABCA2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92339_rabbit-polyclonal-abca2-antibody-42202514264481.jpg%3Ft=1709177288)
![Western Blot: MRP1 Antibody (3W1Y4) [NBP3-16096] - Analysis of extracts of A-549 cells, using MRP1 antibody (NBP3-16096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.](http://images.novusbio.com/fullsize/MRP1-Antibody-3W1Y4-Western-Blot-NBP3-16096-img0003.jpg "Western Blot: MRP1 Antibody (3W1Y4) [NBP3-16096] - Analysis of extracts of A-549 cells, using MRP1 antibody (NBP3-16096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.")
![Immunohistochemistry-Paraffin: MRP1 Antibody (3W1Y4) [NBP3-16096] - Human colon carcinoma using MRP1 antibody (NBP3-16096) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MRP1-Antibody-3W1Y4-Immunohistochemistry-Paraffin-NBP3-16096-img0002.jpg)
![Western Blot: MRP1 Antibody (3W1Y4) [NBP3-16096] - Analysis of extracts of various cell lines, using MRP1 antibody (NBP3-16096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MRP1-Antibody-3W1Y4-Western-Blot-NBP3-16096-img0004.jpg)
![Immunohistochemistry-Paraffin: MRP1 Antibody (3W1Y4) [NBP3-16096] - Human colon using MRP1 antibody (NBP3-16096) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MRP1-Antibody-3W1Y4-Immunohistochemistry-Paraffin-NBP3-16096-img0001.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-20871] - Staining of Human Testis with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP1-20871-img0001.jpg "Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-20871] - Staining of Human Testis with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: PMP70 Antibody [NBP1-20871] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PMP70-Antibody-Flow-Cytometry-NBP1-20871-img0003.jpg)
![Western Blot: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Western blot analysis of NKCC2/SLC12A1 on different lysates with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/1,000 dilution. Lane 1: mouse kidney tissue lysate (no heat) Lane 2: mouse kidney tissue lysate Lane 3: rat kidney tissue lysate Lane 4: K-562 cell lysate (no heat) Tissue lysates at 30 ug/Lane. Cell lysates at 20 ug/Lane. Predicted band size: 121 kDa Observed band size: 150 kDa Exposure time: 1 minutes 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32951) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32951_rabbit-nkcc2-slc12a1-mab-je64-26-288202412442980.jpg "Western Blot: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Western blot analysis of NKCC2/SLC12A1 on different lysates with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/1,000 dilution. Lane 1: mouse kidney tissue lysate (no heat) Lane 2: mouse kidney tissue lysate Lane 3: rat kidney tissue lysate Lane 4: K-562 cell lysate (no heat) Tissue lysates at 30 ug/Lane. Cell lysates at 20 ug/Lane. Predicted band size: 121 kDa Observed band size: 150 kDa Exposure time: 1 minutes 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32951) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32951) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32951_rabbit-nkcc2-slc12a1-mab-je64-26-298202414333656.jpg)
![Immunocytochemistry/ Immunofluorescence: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Immunocytochemistry analysis of K562 cells labeling NKCC2/SLC12A1 with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde and 80% methanol for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32951_rabbit-nkcc2-slc12a1-mab-je64-26-2920241192380.jpg)
![Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775] - Western blot of mouse kidney lysate showing specificimmunolabeling of the ~160 kDa NCC protein phosphorylatedat Thr53 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 min)](http://images.novusbio.com/fullsize/SLC12A3-[p-Thr53]-Antibody-Western-Blot-NBP2-60775-img0001.jpg "Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775] - Western blot of mouse kidney lysate showing specificimmunolabeling of the ~160 kDa NCC protein phosphorylatedat Thr53 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 min)")
![Western Blot: ABCB5 Antibody [NB100-93365] - (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ABCB5-Antibody-Western-Blot-NB100-93365-img0002.jpg "Western Blot: ABCB5 Antibody [NB100-93365] - (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: ABCB5 Antibody [NB100-93365] - analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/ABCB5-Antibody-Flow-Cytometry-NB100-93365-img0001.jpg)
![Western Blot: ABCG4 Antibody [NBP3-41376] - Sample: Recombinant protein.](http://images.novusbio.com/fullsize/nbp3-41376_rabbit-abcg4-pab-612202421262933.jpg "Western Blot: ABCG4 Antibody [NBP3-41376] - Sample: Recombinant protein.")
![Immunohistochemistry-Paraffin: ABCG4 Antibody [NBP3-41376] - DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-41376; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41376_rabbit-abcg4-pab-61220242116286.jpg)
![Western Blot: ABCG4 Antibody [NBP3-41376] - Sample: Human Hela cell lysate; Primary Ab: 1ug/ml NBP3-41376; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41376_rabbit-abcg4-pab-612202421175964.jpg)