F0101B). Quadrants were set based on Mouse IgG1 Flow Cytometry Isotype Control (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrants were set based on Mouse IgG1 Flow Cytometry Isotype Control (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with (A) Human GRP12 or (B) irrelevant transfectant, and eGFP was stained with Mouse Anti-Human GPR12 Monoclonal Antibody (Catalog # MAB6738) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrants were set based on Mouse IgG1 Flow Cytometry Isotype Control (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="GPER was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human GPER Monoclonal Antibody (Catalog # MAB5534) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />