Product Search for erk: Novus Biologicals

AF1018

ERK1/ERK2 [p Thr185, p Tyr187, p Thr202, p Tyr204] Antibody [Unconjugated]

(1 Review)

100 μg

AF1018

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus of the neuron. Protocol available in COMET™ Panel Builder." class="big_lightbox">

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) was detected in immersion fixed paraffin-embedded sections of human Cortex using Rabbit Anti-Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) , Polyclonal Antibody (Catalog #AF1018) at 25ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus of the neuron. Protocol available in COMET™ Panel Builder." alt="Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) was detected in immersion fixed paraffin-embedded sections of human Cortex using Rabbit Anti-Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) , Polyclonal Antibody (Catalog #AF1018) at 25ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus of the neuron. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm of the neuronal processes. Protocol available in COMET™ Panel Builder." class="big_lightbox">

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) was detected in immersion fixed paraffin-embedded sections of mouse Cerebellum using Rabbit Anti-Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187), Polyclonal Antibody (Catalog #AF1018) at 25ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm of the neuronal processes. Protocol available in COMET™ Panel Builder." class="big_thumb" />

HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-105-C, blue line open histogram), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with ice-cold methanol." class="big_lightbox">

<b>Biological Strategies Validation. </b>Jurkat human acute T cell leukemia cell line were untreated (yellow line open histogram) or treated with 200 ng/mL PMA for 15 minutes (filled histogram) and stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) or control antibody (Catalog # <a class=

AB-105-C, blue line open histogram), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with ice-cold methanol." class="big_thumb" />

CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

ERK1/ERK2 was detected in perfusion fixed frozen sections of rat brain (cortex) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. A specific band was detected for ERK1 (T202/Y204)/ERK2 (T185/Y187) at approximately 44 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Biochemical analysis of ErbB signaling.Immunoblot analysis performed on P90 kidneys from Cdh16Cre::Tfebfs mice (A) and P90 Cdh16CreErt2::Tfebfs animals induced with tamoxifen at P14 (B) and at P30 (C), respectively. Each replicate is a different biological sample. ErbB was analyzed by quantifying phosphoAKT (Ser473) to total AKT, and phosphoERK1 (T202/Y204)/ERK2(T185/Y187) to total ERK; graphs are the densitometry quantifications of Western blot bands normalized to wild-type line and are shown as an average (± SEM) (*p<0.05, **p<0.01, ***p<0.001, two-sided Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.011 Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/17047), licensed under a CC-BY license. Not internally tested by R&D Systems.

Activation of ErbB and WNT signaling pathways in kidneys from Cdh16Cre::Tfebfs mice.Transcriptional and biochemical analyses were performed on Cdh16Cre and Cdh16Cre::Tfebfs mice. (A,B) Tables show the relative increase of genes related to the ErbB (A) and WNT (B) pathways in the microarray analyses performed on kidneys from P0 Cdh16Cre::Tfebfs mice. Graphs show real-time PCR validations performed on kidneys from Cdh16Cre::Tfebfs mice at different stages (P0, P12, P30). Data are shown as the average (± SEM) of at least three Cdh16Cre::Tfebfs mice normalized versus wild-type mice. (C,D) Immunoblot analyses performed on (C) P30 kidney tissues and (D) primary kidney cells isolated from Cdh16Cre::Tfebfs mice to evaluate ErbB and WNT activation status. Each replicate is a distinct biological sample. ErbB signaling was assessed by looking at phosphoAKT (Ser473) to total AKT ratio, and phosphoERK1 (T202/Y204)/ERK2(T185/Y187) to total ERK ratio; WNT signaling was assessed by quantifying beta -catenin and CCND1 (Cyclin D1) protein levels. Graphs represent the densitometry quantification of Western blot bands. Values are normalized to actin when not specified and are shown as an average (± SEM) (*p<0.05, **p<0.01, ***p<0.001, two-sided, Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.00710.7554/eLife.17047.008Figure 3—source data 1.Complete list of 294 genes (represented by 361 probesets) significantly induced (FDR≤0.05) in the KSP_P0 microarray dataset (GSE62977).The genes are ranked by decreasing signed ratio (KSP_P0/CTL).DOI://dx.doi.org/10.7554/eLife.17047.00810.7554/eLife.17047.009Figure 3—source data 2.Complete list of 628 genes (represented by 729 probesets) significantly induced (FDR≤0.05) in the KSP_P14 microarray dataset (GSE63376).The genes are ranked by decreasing signed ratio (KSP_P14/CTL).DOI://dx.doi.org/10.7554/eLife.17047.009Complete list of 294 genes (represented by 361 probesets) significantly induced (FDR≤0.05) in the KSP_P0 microarray

<b>Biological Strategies Validation. </b> beta -blockers inhibit the proliferation of breast cancer cell lines(A) A dose curve of propranolol was administered to a panel of breast cancer cell lines and normal primary mammary epithelial cells (HMECS) and viability was assessed. (B) Immunofluorescent detection of Ki-67 protein expression in control or propranolol-treated (18 μM) SK-BR-3 breast cancer cells after 24 hours. Hoechst 33342 was used as a nuclear counterstain. (C) Heatmap depicting phospho-MAPK antibody array results testing the status of 24 kinases in SK-BR-3 cells subjected to 24 hours control or propranolol (18 μM). (red = upregulated; green = downregulated; black = no detectable expression). (D) Western blot confirmation of the antibody array results in SK-BR-3 cells subjected to 24 hours control or propranolol (18 μM). (D) Western blot analysis of cell lysates from SK-BR-3 cells subjected to 24 hours control or propranolol (18 μM), confirming the phosphorylation events identified in the antibody array. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.14119), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1018U-100UG
Alexa Fluor 405	AF1018V-100UG
Alexa Fluor 488	AF1018G-100UG
Alexa Fluor 532	AF1018X-100UG
Alexa Fluor 594	AF1018T-100UG
Alexa Fluor 647	AF1018R-100UG
Alexa Fluor 700	AF1018N-100UG
Alexa Fluor 750	AF1018S-100UG
Alexa Fluor Plus 405	AF1018AFP405-100UG
Alexa Fluor Plus 488	AF1018AFP488-100UG
Alexa Fluor Plus 555	AF1018AFP555-100UG
Alexa Fluor Plus 594	AF1018AFP594-100UG
Alexa Fluor Plus 647	AF1018AFP647-100UG
Alexa Fluor Plus 680	AF1018AFP680-100UG
Allophycocyanin	AF1018A
Biotin	AF1018B
CoraFluor 1	AF1018CL1
DyLight 350	AF1018D
DyLight 405	AF1018E
DyLight 488	AF1018K
DyLight 550	AF1018L
DyLight 594	AF1018M
DyLight 650	AF1018W
DyLight 680	AF1018Y
DyLight 755	AF1018Z
HRP	AF1018H
Janelia Fluor 525	AF1018JF525
Janelia Fluor 549	AF1018I
Janelia Fluor 646	AF1018J
Janelia Fluor 669	AF1018JF669
PE	AF1018P
PerCP	AF1018C
mFluor Violet 450 SE	AF1018MFV450
mFluor Violet 500 SE	AF1018MFV500
mFluor Violet 610 SE	AF1018MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1018-SP

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AF1576

ERK1/ERK2 Antibody [Unconjugated]

(1 Review)

(36 Publications)

100 μg

AF1576

HAF008). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat ERK1/ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat ERK1/ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for ERK1/ERK2 at approximately 44 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK1/ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1576). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>Phospho-proteome profiling results of LX-2 cells co-cultured with C3A–CYP2E1 cells with or without DDC treatment and detection of the effect of DDC on intracellular kinases in LX-2 cells of co-cultures200 μg of total cell lysates from LX-2 cells co-cultured with C3A-2E1 cells with or without 100 μM DDC for 1 h were incubated with membranes of the human phospho-MAPK Array Kit according to the manufacturer's instructions. Phospho MAPK Array data were developed on X-ray films following exposure to chemiluminescent reagents. 20 μg aliquots of total cell lysates from LX-2 cells were subjected to Western blotting analysis. (A) Template showing the location of MAPK antibodies spotted onto the human phospho-MAPK Array Kit. (B) The activation status of ERK1/2 in LX-2 cells co-cultured with C3A-2E1 cells after DDC treatment. (C) The activation status of p38 in LX-2 cells co-cultured with C3A-2E1 cells after DDC treatment. (D) The activation status of Akt in LX-2 cells co-cultured with C3A-2E1 cells after DDC treatment. (E) The activation status of ERK1/2, p38 and Akt in LX-2 cells co-cultured with C3A cells after DDC treatment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>DDC up-regulates MMP-1 through ERK1/2 and Akt activation(A) Co-cultures were treated with 100 μM DDC for the indicated time. Phosphorylation of ERK1/2, p38 and Akt were determined by Western blotting analysis. The corresponding non-phosphorylated ERK1/2, p38, Akt and beta -actin were used for protein loading control. (B) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of ERK1/2 inhibitor (U0126, 10 μM). MMP-1 protein levels were analysed by Western blotting. (C) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of p38 inhibitor (SB203580, 10 μM). MMP-1 protein levels were analysed by Western blotting. (D) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of Akt inhibitor (T3830, 50 μM). MMP-1 protein levels were analysed by Western blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>p38 inhibitor SB203580 improves the up-regulation of MMP-1 by DDC through stimulating ERK1/2Co-cultures were treated with or without 100 μM DDC for 1 h in the presence or absence of p38 inhibitor (SB203580, 10 μM). Phosphorylation of ERK1/2, p38 and Akt in LX-2 cells of co-cultures were determined by Western blotting analysis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Depletion of Rac1b increases constitutive and growth factor-induced ERK1/2 phosphorylation. (A) Panc1 cells were transiently transfected with siRNA specific to Rac1b (R1b) or irrelevant control siRNA (Co), serum-starved for 24 h and treated with TGF-beta 1 as indicated. Cells were subjected to immunoblotting for p-ERK1/2, t-ERK1/2 and HSP90 as well as Rac1b as a control for transfection efficiency. The chart below shows the relative intensities of the p-ERK1/2 bands normalized to those for t-ERK1/2 from three independent experiments (mean ± SD, n = 3). Data are displayed relative to non-stimulated Rac1b siRNA-transfected cells set arbitrarily at 1. Asterisks indicate a significant difference. (B) Colo357 and IMIM-PC1 cells were transiently transfected twice on two consecutive days with 50 nM each of a siRNA specific for Rac1b (R1b), or an irrelevant control siRNA (Co), serum-starved for 24 h and subjected to immunoblotting for phospho-ERK1/2 (p-ERK1/2) and total ERK1/2 (t-ERK1/2) as well al HSP90 as a loading control. The charts below the blots show the relative intensity of the p-ERK1/2 bands normalized to the t-ERK1/2 bands of three independent experiments (mean ± SD, n = 3). Asterisks indicate a significant difference. (C) Panc1 cells stably expressing a dominant negative ALK5 mutant (ALK5KR) or empty vector were transfected twice with 50 nM each of Co siRNA or Rac1b siRNA and subjected to immunoblot analysis of p-ERK1/2. The blot was stripped and reprobed with antibodies against and t-ERK1/2 and Rac1b. The graph below the blots show the relative intensities of p-ERK1/2 normalized to those for t-ERK1/2 from three independent experiments (mean ± SD, n = 3). (D) Panc1 cells were transfected with 50 nM of irrelevant Co siRNA, Rac1b siRNA or ALK5 siRNA, or 25 nM each of Rac1b + ALK5 siRNA. Cells were serum-starved, treated with TGF-beta 1 for 12 h and subjected to immunoblotting for p-ERK1/2, t-ERK1/2 and HSP90 as well as for Rac1b and ALK5 to verify successful depletion. Th

<b>Biological Strategies Validation. </b>Effects of ectopic overexpression of Rac1b on TGF-beta 1-induced ERK1/2 activation and gene expression. (A) Panc1 cells stably overexpressing Rac1b from the pCGN vector (two independent clones, #4 and #13) as well as empty-vector control cells (v) were serum-starved, treated with TGF-beta 1 for 12 h and subjected to immunoblotting for p-ERK1/2, t-ERK1/2, and HA-Rac1b. The graph underneath the blot shows results from densitometric analysis of the respective bands for p-ERK1/2 normalized to those for t-ERK1/2 and derived from four independent experiments (mean ± SD, n = 4). Data are displayed relative to TGF-beta 1 stimulated vector control cells set arbitrarily at 1. Asterisks indicate a significant difference of p-ERK1/2 band intensity between the vector control and the two HA-Rac1b expressing clones. Due to the short exposure time, endogenous Rac1b protein is not visible. (B) The same cells as in (A) were stimulated with TGF-beta 1 for 24 h and subjected to qPCR for E-cadherin and PAR2. Data are displayed as TGF-beta 1-treated over control cells and are the mean ± SD of 3 experiments. Asterisks indicate significant differences. Data are from the same blot/gel treated subsequently with antibodies to p-ERK1/2, Rac1b, and HSP90, then stripped and reincubated with an antibody to t-ERK1/2. The vertical lines between lanes 3 and 4 indicate removal of irrelevant lanes from the blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-15170-6), licensed under a CC-BY license. Not internally tested by R&D Systems.

CREG overexpression activates both ERK&PI3K/Akt signaling pathways,&ERK, but not PI3K/Akt, mediates CREG effects on HUVEC proliferation. (A) In the three groups of cells (HUVEC, AdGFP&AdCREG), expression of JNK, p38, ERK, PI3K&Akt signaling molecules&their phosphorylated (p-) forms detected by WB, with beta -tubulin as the loading control. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24018888), licensed under a CC-BY license. Not internally tested by R&D Systems.

ERK, but not PI3K/Akt, mediates the increase of cyclin E induced by CREG overexpression in HUVEC. (A) Expression of p-ERK, ERK, p-Akt, Akt, cyclin E and beta -tubulin was detected by Western blotting in the three groups of cells with LY294002 (50 μM) or PD098059 (20 μM) treatment. Vehicle (DMSO)-treated cells were used as control, and beta -tubulin was used as a loading control; (B,C) Pooled analysis of the levels of Akt (B) and ERK (C) signaling activation accessed by the grayscale ratios of p-Akt/Akt and p-ERK/ERK, respectively. Data are given as the mean ± SD (n = 3). *p < 0.05; **p < 0.01. NS: no significant difference. (D) After normalization to beta -tubulin, the difference in the expression of cyclin E between the HUVEC-AdGFP and HUVEC-AdCREG group was reduced by the treatment of PD098059, but not LY294002. Data are given as the mean ± SD (n = 3). **p < 0.01. NS: no significant difference. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24018888), licensed under a CC-BY license. Not internally tested by R&D Systems.

Image of total ERK in A549 cells. Primary antibody dilution - 1:20 in antibody diluent 2. 60 min of incubation. Image from a verified customer review.

rpS6 activity is preserved and MAPK1/3 and p90S6K are activated in the striatum of R6/1 mice after prolonged treatment with PPX. Western blot for the total and phosphorylated forms of rpS6 at Ser235/236 (A), MAPK1/3 at Thr222/Tyr204 (B), and p90S6K at Ser380 (C) in WT and R6/1 mice. The densitometric analysis showed that Ser235/236-rpS6 phosphorylation was unaffected (A) and that PPX induced phosphorylations of Thr202/Tyr204-MAPK1/3 (B) and Ser380-p90S6K (C) in R6/1 mice. No changes were detected in WT mice. n = 6–12 mice per experimental group, unpaired t-test; ns = non-significant; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40358175), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1576U-100UG
Alexa Fluor 405	AF1576V-100UG
Alexa Fluor 488	AF1576G-100UG
Alexa Fluor 532	AF1576X-100UG
Alexa Fluor 594	AF1576T-100UG
Alexa Fluor 647	AF1576R-100UG
Alexa Fluor 700	AF1576N-100UG
Alexa Fluor 750	AF1576S-100UG
Alexa Fluor Plus 405	AF1576AFP405-100UG
Alexa Fluor Plus 488	AF1576AFP488-100UG
Alexa Fluor Plus 555	AF1576AFP555-100UG
Alexa Fluor Plus 594	AF1576AFP594-100UG
Alexa Fluor Plus 647	AF1576AFP647-100UG
Alexa Fluor Plus 680	AF1576AFP680-100UG
Allophycocyanin	AF1576A
Biotin	AF1576B
CoraFluor 1	AF1576CL1
DyLight 350	AF1576D
DyLight 405	AF1576E
DyLight 488	AF1576K
DyLight 550	AF1576L
DyLight 594	AF1576M
DyLight 650	AF1576W
DyLight 680	AF1576Y
DyLight 755	AF1576Z
FITC	AF1576F
HRP	AF1576H
Janelia Fluor 525	AF1576JF525
Janelia Fluor 549	AF1576I
Janelia Fluor 646	AF1576J
Janelia Fluor 669	AF1576JF669
PE	AF1576P
PerCP	AF1576C
mFluor Violet 450 SE	AF1576MFV450
mFluor Violet 500 SE	AF1576MFV500
mFluor Violet 610 SE	AF1576MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1576-SP

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(select up to 3 products from this page to compare)

MAB1018

ERK1/ERK2 [p Tyr187, p Tyr204, p Thr185, p Thr202] Antibody (269434) [Unconjugated]

(21 Publications)

100 μg

MAB1018

120-HD) and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta -NGF (Catalog # 556-NG) and HeLa human cervical epithelial carcinoma cell line untreated or treated with 200 nM PMA. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Monoclonal Antibody (MAB1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Rabbit Anti-Human PDGF (Catalog # <a class=

120-HD) and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta -NGF (Catalog # 556-NG) and HeLa human cervical epithelial carcinoma cell line untreated or treated with 200 nM PMA. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Monoclonal Antibody (MAB1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Rabbit Anti-Human PDGF (Catalog # 120-HD) and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta -NGF (Catalog # 556-NG) and HeLa human cervical epithelial carcinoma cell line untreated or treated with 200 nM PMA. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Monoclonal Antibody (MAB1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>ERK1/ERK2 phosphorylated at T202/Y204 (ERK1) and T185/Y187 (ERK2) was detected in immersion fixed Hela human cervical epithelial carcinoma cells, unstimulated (lower panel) and stimulated (upper panel) with PMA using Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Monoclonal Antibody (Catalog # MAB1018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # <a class=

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

AB-105-C, blue open histogram), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol." class="big_lightbox">

<b>Biological Strategies Validation. </b>Jurkat human acute T cell leukemia cell line were unstimulated (light orange open histogram) or treated with 50 ng/mL PMA for 10 minutes (dark orange filled histogram), then stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Monoclonal Antibody (Catalog # MAB1018) or control antibody (Catalog # <a class=

AB-105-C, blue open histogram), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol." class="big_thumb" />

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 200 nM PMA and Ionomycin for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) at approximately 42 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Monoclonal Antibody (Catalog # MAB1018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>Differentiation Characteristics of Individual Stem Cells.(A) Flow cytometric analysis of undifferentiated stem cell markers in the clonal NTERA2.Tom cell line. Black line depicts P3X negative control and green depicts antigen specific expression (OCT4, SOX2, NANOG and SSEA4). (B) Graph depicting the percentage of TUJ1 positive neurons within differentiated colonies derived from individual RA treated NTERA2.Tom stem cells. (C) Images of differentiated NTERA2.Tom colonies derived from individual NTERA2.Tom cells on a background of wildtype NTERA2 cells. Cell were exposed to RA for 21 days (Green - beta III tubulin, Blue - Hoechst) White arrows depict beta III tubulin positive NTERA2.Tom neurons. Scale bar represents 50 µM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20531938), licensed under a CC-BY license. Not internally tested by R&D Systems.

Müller cells were pre-incubated with TIMP-3 (100 ng/ml) or dilution medium for 1 h before increasing the sugar content of the cultures [25 mM of mannitol as control or 25 mM of glucose (HG)]. After 24 h levels of the p65 subunit of NF-kappa B [panel (A)], phospho-ERK1/2 [panel (B)], caspase-3 [panel (C)], and ADAM17 [panel (D)] in cell lysates was determined by Western blot analysis. Results are expressed as median (interquartile range) from three different experiments performed in triplicate. Kruskal-Wallis test and Mann-Whitney test were used for comparisons between three groups and two groups, respectively. *p < 0.05 compared with values obtained from cells treated with mannitol. #p < 0.05 compared with values obtained from cells treated with HG. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35082694), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC1018U-100UG
Alexa Fluor 405	IC1018V-100UG
Alexa Fluor 488	IC7806G-100UG
Alexa Fluor 532	FAB1018X
Alexa Fluor 594	IC1018T-100UG
Alexa Fluor 647	IC1018R-100UG
Alexa Fluor 700	IC1018N-100UG
Alexa Fluor 750	IC1018S-100UG
Alexa Fluor Plus 405	FAB1018AFP405-100UG
Alexa Fluor Plus 488	FAB1018AFP488-100UG
Alexa Fluor Plus 555	FAB1018AFP555-100UG
Alexa Fluor Plus 594	FAB1018AFP594-100UG
Alexa Fluor Plus 647	FAB1018AFP647-100UG
Alexa Fluor Plus 680	FAB1018AFP680-100UG
Allophycocyanin	FAB1018A
Biotin	FAB1018B
CoraFluor 1	FAB1018CL1
DyLight 350	FAB1018D
DyLight 405	FAB1018E
DyLight 488	FAB1018K
DyLight 550	FAB1018L
DyLight 594	FAB1018M
DyLight 650	FAB1018W
DyLight 680	FAB1018Y
DyLight 755	FAB1018Z
FITC	FAB1018F
HRP	FAB1018H
Janelia Fluor 525	FAB1018JF525
Janelia Fluor 549	FAB1018I
Janelia Fluor 646	FAB1018J
Janelia Fluor 669	FAB1018JF669
PE	FAB1018P
PerCP	FAB1018C
mFluor Violet 450 SE	FAB1018MFV450
mFluor Violet 500 SE	FAB1018MFV500
mFluor Violet 610 SE	FAB1018MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC7806G-100UG
Carrier Free	MAB1018-SP
Carrier Free	IC1018V-100UG
Carrier Free	IC1018U-100UG
Carrier Free	IC1018T-100UG
Carrier Free	IC1018S-100UG
Carrier Free	IC1018R-100UG
Carrier Free	IC1018N-100UG

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MAB1576

ERK1/ERK2 Antibody (216703) [Unconjugated]

(3 Reviews)

(19 Publications)

100 μg

MAB1576

HAF007). For additional reference, recombinant human ERK1 and ERK2 (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44/42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). For additional reference, recombinant human ERK1 and ERK2 (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44/42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ERK1 and ERK2 (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44/42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

ERK1/ERK2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1576U-100UG
Alexa Fluor 405	FAB1576V-100UG
Alexa Fluor 488	FAB1576G-100UG
Alexa Fluor 532	FAB1576X-100UG
Alexa Fluor 594	FAB1576T-100UG
Alexa Fluor 647	FAB1576R-100UG
Alexa Fluor 700	FAB1576N-100UG
Alexa Fluor 750	FAB1576S-100UG
Alexa Fluor Plus 405	FAB1576AFP405-100UG
Alexa Fluor Plus 488	FAB1576AFP488-100UG
Alexa Fluor Plus 555	FAB1576AFP555-100UG
Alexa Fluor Plus 594	FAB1576AFP594-100UG
Alexa Fluor Plus 647	FAB1576AFP647-100UG
Alexa Fluor Plus 680	FAB1576AFP680-100UG
Allophycocyanin	FAB1576A
Biotin	FAB1576B
CoraFluor 1	FAB1576CL1
DyLight 350	FAB1576D
DyLight 405	FAB1576E
DyLight 488	FAB1576K
DyLight 550	FAB1576L
DyLight 594	FAB1576M
DyLight 650	FAB1576W
DyLight 680	FAB1576Y
DyLight 755	FAB1576Z
FITC	FAB1576F
HRP	FAB1576H
Janelia Fluor 525	FAB1576JF525
Janelia Fluor 549	FAB1576I
Janelia Fluor 646	FAB1576J
Janelia Fluor 669	FAB1576JF669
PE	FAB1576P
PerCP	FAB1576C
mFluor Violet 450 SE	FAB1576MFV450
mFluor Violet 500 SE	FAB1576MFV500
mFluor Violet 610 SE	FAB1576MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1576-SP

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MAB15761

ERK1/ERK2 Antibody (631122) [Unconjugated]

(1 Review)

(3 Publications)

100 μg

MAB15761

HAF007). Specific bands were detected for ERK1/ERK2 at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of MCF-7 human breast cancer cell line, BaF3 mouse pro-B cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB15761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). Specific bands were detected for ERK1/ERK2 at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, BaF3 mouse pro-B cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB15761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for ERK1/ERK2 at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB15761U-100UG
Alexa Fluor 405	FAB15761V-100UG
Alexa Fluor 488	FAB15761G-100UG
Alexa Fluor 532	FAB15761X-100UG
Alexa Fluor 594	FAB15761T-100UG
Alexa Fluor 647	FAB15761R-100UG
Alexa Fluor 700	FAB15761N-100UG
Alexa Fluor 750	FAB15761S-100UG
Alexa Fluor Plus 405	FAB15761AFP405-100UG
Alexa Fluor Plus 488	FAB15761AFP488-100UG
Alexa Fluor Plus 555	FAB15761AFP555-100UG
Alexa Fluor Plus 594	FAB15761AFP594-100UG
Alexa Fluor Plus 647	FAB15761AFP647-100UG
Alexa Fluor Plus 680	FAB15761AFP680-100UG
Allophycocyanin	FAB15761A
Biotin	FAB15761B
CoraFluor 1	FAB15761CL1
DyLight 350	FAB15761D
DyLight 405	FAB15761E
DyLight 488	FAB15761K
DyLight 550	FAB15761L
DyLight 594	FAB15761M
DyLight 650	FAB15761W
DyLight 680	FAB15761Y
DyLight 755	FAB15761Z
FITC	FAB15761F
HRP	FAB15761H
Janelia Fluor 525	FAB15761JF525
Janelia Fluor 549	FAB15761I
Janelia Fluor 646	FAB15761J
Janelia Fluor 669	FAB15761JF669
PE	FAB15761P
PerCP	FAB15761C
mFluor Violet 450 SE	FAB15761MFV450
mFluor Violet 500 SE	FAB15761MFV500
mFluor Violet 610 SE	FAB15761MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB15761-SP

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MAB1825

ERK1/ERK2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (798508) [Unconjugated]

(2 Publications)

100 μg

MAB1825

220-BB) for 5 minutes and 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB1825) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Phospho-ERK1 (T202/Y204) and Phospho-ERK2 (T185/Y187) at approximately 43 and 41 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human PDGF-BB (Catalog # <a class=

220-BB) for 5 minutes and 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB1825) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Phospho-ERK1 (T202/Y204) and Phospho-ERK2 (T185/Y187) at approximately 43 and 41 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 5 minutes and 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB1825) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Phospho-ERK1 (T202/Y204) and Phospho-ERK2 (T185/Y187) at approximately 43 and 41 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1825U-100UG
Alexa Fluor 405	FAB1825V-100UG
Alexa Fluor 488	FAB1825G-100UG
Alexa Fluor 532	FAB1825X-100UG
Alexa Fluor 594	FAB1825T-100UG
Alexa Fluor 647	FAB1825R-100UG
Alexa Fluor 700	FAB1825N-100UG
Alexa Fluor 750	FAB1825S-100UG
Alexa Fluor Plus 405	FAB1825AFP405-100UG
Alexa Fluor Plus 488	FAB1825AFP488-100UG
Alexa Fluor Plus 555	FAB1825AFP555-100UG
Alexa Fluor Plus 594	FAB1825AFP594-100UG
Alexa Fluor Plus 647	FAB1825AFP647-100UG
Alexa Fluor Plus 680	FAB1825AFP680-100UG
Allophycocyanin	FAB1825A
Biotin	FAB1825B
CoraFluor 1	FAB1825CL1
DyLight 350	FAB1825D
DyLight 405	FAB1825E
DyLight 488	FAB1825K
DyLight 550	FAB1825L
DyLight 594	FAB1825M
DyLight 650	FAB1825W
DyLight 680	FAB1825Y
DyLight 755	FAB1825Z
FITC	FAB1825F
HRP	FAB1825H
Janelia Fluor 525	FAB1825JF525
Janelia Fluor 549	FAB1825I
Janelia Fluor 646	FAB1825J
Janelia Fluor 669	FAB1825JF669
PE	FAB1825P
PerCP	FAB1825C
mFluor Violet 450 SE	FAB1825MFV450
mFluor Violet 500 SE	FAB1825MFV500
mFluor Violet 610 SE	FAB1825MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1825-SP

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NBP3-26412

ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) - BSA Free

100 ul

NBP3-26412

Immunocytochemistry/Immunofluorescence: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Staining of Hela cells with ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-26412-50ul

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NBP3-23530

ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free

100 ul

NBP3-23530

<b>Biological Strategies Validation. </b>Western Blot: ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free [NBP3-23530] - Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100nM, 30 min; +), using NBP3-23530 at 1:1000 dilution.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-23530-20ul

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NBP3-21670

ERK1/ERK2 Antibody (SR1700) - BSA Free

100 ul

NBP3-21670

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21670-50ul

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NBP3-21588

ERK1/ERK2 [p Thr202] Antibody (SR1701) - BSA Free

100 ul

NBP3-21588

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21588-50ul

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NBP3-13137

ERK1/ERK2 [p Thr185, p Tyr187] Antibody

100 ul

NBP3-13137

<b>Biological Strategies Validation. </b>Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) / ERK2 (phospho Thr185/Tyr187) antibody (NBP3-13137) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-50357

ERK1/ERK2 [p Thr202] Antibody (23GB4515)

100 ul

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

ERK1/ERK2 [p Thr185, p Tyr187, p Thr202, p Tyr204] Antibody [Unconjugated]

ERK1/ERK2 Antibody [Unconjugated]

ERK1/ERK2 [p Tyr187, p Tyr204, p Thr185, p Thr202] Antibody (269434) [Unconjugated]

ERK1/ERK2 Antibody (216703) [Unconjugated]

ERK1/ERK2 Antibody (631122) [Unconjugated]

ERK1/ERK2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (798508) [Unconjugated]

ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) - BSA Free

ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free

ERK1/ERK2 Antibody (SR1700) - BSA Free

ERK1/ERK2 [p Thr202] Antibody (SR1701) - BSA Free

ERK1/ERK2 [p Thr185, p Tyr187] Antibody

ERK1/ERK2 [p Thr202] Antibody (23GB4515)