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NB500-201

Survivin Antibody - BSA Free

(22 Reviews)

(380 Publications)

0.1 ml

NB500-201

<b>Biological Strategies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Survivin-Antibody-Knockdown-Validated-NB500-201-img0037.jpg

Survivin-Antibody-Immunohistochemistry-NB500-201-img0038.jpg

<b>Genetic Strategies Validation. </b>Survivin-Antibody-Knockdown-Validated-NB500-201-img0033.jpg

Immunocytochemistry/Immunofluorescence: Survivin Antibody [NB500-201] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using [NB500-201].

Western Blot: Survivin Antibody [NB500-201] - Analysis of 30ug of HeLa whole cell lysate [NB800-PC1] using rabbit polyclonal [NB500-201] at 1ug/ml. Detection was performed using ECL method with 1 minute exposure. Band detected at higher molecular weight than the predicted MW (16 kDa).

Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Immunohistochemical staining of Survivin in human rectal cancer using [NB500-201] and DAB with hematoxylin counterstain.

Flow Cytometry: Survivin Antibody [NB500-201] - An intracellular stain was performed on HeLa cells with [NB500-201] and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.

Western Blot: Survivin Antibody [NB500-201] - Analysis of Survivin in human hepatocytes from cancer patient (left) and HeLa cell lysate (right) using [NB500-201]. Image from verified customer review. Note: bands detected at higher molecular weight than predicted (16 kDa)

<b>Biological Strategies Validation. </b>Survivin-Antibody-Western-Blot-NB500-201-img0028.jpg

Immunocytochemistry/Immunofluorescence: Survivin Antibody [NB500-201] - Analysis of HeLa cells using Survivin Antibody ([NB500-201], 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Analysis of Survivin in ovarian cancer tissue using [NB500-201]. Image from verified customer review.

Immunohistochemistry: Survivin Antibody [NB500-201] - HRP conjugated Survivin expression in BIRC5 transfected 293T cells using Survivin Antibody [NB500-201]. Image from verified customer review.

Immunohistochemistry: Survivin Antibody [NB500-201] - Immunohistochemical analysis using [NB500-201]. The top photo is a control stain and the bottom photo is anti-survivin staining of melanoma. Photo courtesy of Dr. Dario Altieri, Yale University.

Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Survivin shows lysates of human neuroblastoma cell line. Polyvinylidene fluoride (PVDF) membrane was probed with 1:200 dilution of 0.5 ug/mL of rabbit polyclonal [NB500-201], followed by 1:2000 dilution of goat anti-rabbit IgG.

Simple Western: Survivin Antibody [NB500-201] - Simple Western analysis using [NB500-201]. Lane view shows a specific band for Survivin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Theoretical molecular weight: 16 kDa.

Radiation-resistant Caveolin-1 (CAV1)-silenced fibroblasts differentially express and secrete the apoptosis inhibiting protein TP53-regulated inhibitor of apoptosis 1 (TRIAP1).

Expression of survivin protein in cell lines after transfection with siRNA.Survivin protein expression in CHS cell lines was evaluated by western blotting at 48 h after transfection with scrambled and survivin siRNA.

<b>Biological Strategies Validation. </b>Western blotting analysis in MDA-MB-231 cells and SUM 149 cells.The cells were treated for 24 h with C. juttae essential oil (EO) (46 and 64 μg/ml, respectively). The data shown are the results of a representative experiment.

(A) Levels of suppression of Survivin and expression of p53 for 2 h, 4 h and 8 h for MBA-MD 231 cells. (B) Light microscopy images of the three cell types using nanoconstruct with the AS1411 aptamer. Cell population had been observed to reduce for the MBA-MD 231 cells and AGS while the non-tumorigenic cells MCF-10a did not exhibit any appreciable loss in cell number as well as cell morphology.

Detection of BCL2, Bcl-xL, XIAP and survivin protein content by western blotting 48 h after transfection with a total of 40 nM siRNA in EJ28 bladder cancer cells. Beta-actin was used for loading control.

$Western Blot: Survivin Antibody - BSA Free [NB500-201] - Deficiency in CCAR2 or Hsp60 reduces expression of survivin. SH-SY5Y cells were transfected with Universal (siU), CCAR2 (siC), or Hsp60 (siH) siRNA. Forty-eight hours later, expression of survivin protein was examined by western blotting. (A) Survivin expression was detected in whole cell lysates. The relative level of survivin protein is presented as the mean ± standard error of the mean (SEM) (n = 3). Asterisks (*) denote statistically significant differences (p < 0.05, one-way ANOVA). (B) Cytosolic & mitochondrial fractions were isolated to determine localization & expression of survivin. (C) Two different siRNAs specific for CCAR2 & Hsp60 were used to knock down their expressions. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30609639), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Survivin & BIM expression in response to lapatinibWestern analysis of Survivin & BIM protein levels after 24 hour exposure to 0.1% DMSO (−) or 2 μM lapatinib (+) in lapatinib-resistant cells (A), cells overexpressing t-Darpp (B), or SK/HerR cells transiently transfected with siRNA targeted to GFP (siCtrl) or Darpp-32/t-Darpp (siDp) for 72 hours (C). alpha -Tubulin was used as a loading control. Protein expression was quantified using ImageJ software. Data was normalized to alpha -Tubulin levels & expressed as the fold change in protein level after lapatinib treatment, relative to the DMSO control, for each cell line. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.5311), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Expression of stem cell & differentiation markers in RAD, NRAD & TOT cells from cSCC. (A) Cells were analyzed immediately after separation, & levels of markers were determined by Western blot analysis. beta -actin was used as loading control; (B) Bar graphs show the average densitometry values normalized to actin. *p < 0.05; **p < 0.01. Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/10/19540), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Expression of survivin protein in cell lines after transfection with siRNA.Survivin protein expression in CHS cell lines was evaluated by western blotting at 48 h after transfection with scrambled & survivin siRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260303), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Survivin protein stability is increased in the presence of autophagy inhibitors in serum-free media, but not in serum-containing media. Western blots & protein quantitation graphs showing survivin protein levels over time after addition of CHX (100 μM) to media. One representative western blot out of three repeat experiments was shown for each treatment & condition. The prominent band shown is survivin while the smaller bands are nonspecific, as determined via control experiments utilizing a neutralizing peptide for the survivin antibody. *Indicates the time at which survivin protein is half of the amount at 0 hours (half-life), P < 0.02. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23431290), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - (A) Levels of suppression of Survivin & expression of p53 for 2 h, 4 h & 8 h for MBA-MD 231 cells. (B) Light microscopy images of the three cell types using nanoconstruct with the AS1411 aptamer. Cell population had been observed to reduce for the MBA-MD 231 cells & AGS while the non-tumorigenic cells MCF-10a did not exhibit any appreciable loss in cell number as well as cell morphology. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-00912-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Survivin Antibody - BSA Free [NB500-201] - Deficiency in CCAR2 or Hsp60 reduces expression of survivin. SH-SY5Y cells were transfected with Universal (siU), CCAR2 (siC), or Hsp60 (siH) siRNA. Forty-eight hours later, expression of survivin protein was examined by western blotting. (A) Survivin expression was detected in whole cell lysates. The relative level of survivin protein is presented as the mean ± standard error of the mean (SEM) (n = 3). Asterisks (*) denote statistically significant differences (p < 0.05, one-way ANOVA). (B) Cytosolic & mitochondrial fractions were isolated to determine localization & expression of survivin. (C) Two different siRNAs specific for CCAR2 & Hsp60 were used to knock down their expressions. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30609639), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Immunohistochemistry: Survivin Antibody - BSA Free [NB500-201] - RAD & NRAD-derived tumor characterization. (A) Mitotic Index representing the number of cells undergoing mitosis over total cells were counted in RAD & NRAD-derived tumors **p < 0.01; (B) Survivin & Ki67 expression in RAD & NRAD-derived tumors by immunohistochemistry. Scale bar = 200 μm; (C) K10, E-FABP & involucrin expression in RAD & NRAD-derived tumors by immunohistochemistry. Scale bar = 200 μm. Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/10/19540), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Gene-silencing of Bcl-2, Survivin & XIAP. Western blot showed efficient silencing in transfected MiaPaCa-2 (A) & AsPC-1 cells (B). 50,000 cells/well were single-transfected with carrier solution (lane 1) & siRNA against Luciferase (lane 2) as control. SGS, lowST & ST all effectively silenced the three target genes (lane 3-5). Efficient knock-down was also shown in the lowST group by RT-PCR in MiaPaCa-2 (C) & AsPC-1 cells (D). White bars show controls, grey bars signify transfected cells. All samples were normalized to beta -Actin as a house-keeping gene. SGS = Simultaneous gene silencing; lowST = Low dose siRNA transfection; ST = Standard dose siRNA transfection. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20646298), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Survivin Antibody - BSA Free [NB500-201] - CCAR2 binds Hsp60 & survivin. Interaction between CCAR2 & survivin was examined in SH-SY5Y or HEK293 cells by co-immunoprecipitation with either an anti-CCAR2 or an anti-survivin antibody, followed by western blotting. (A) Interaction between CCAR2 & survivin in whole cell lysates from SH-SY5Y cells was examined. (B) Interaction between CCAR2, survivin, & Hsp60 in cytosolic & mitochondrial fractions isolated from HEK293 cells was examined. (C) SH-SY5Y cells were depleted of Hsp60 & the interaction between CCAR2 & survivin was examined. siU, universal siRNA; siH, Hsp60 siRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30609639), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Radiation-resistant Caveolin-1 (CAV1)-silenced fibroblasts differentially express & secrete the apoptosis inhibiting protein TP53-regulated inhibitor of apoptosis 1 (TRIAP1). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30871022), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Western blotting analysis in MDA-MB-231 cells & SUM 149 cells.The cells were treated for 24 h with C. juttae essential oil (EO) (46 & 64 μg/ml, respectively). The data shown are the results of a representative experiment. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30921428), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Overexpression of Survivin predominantly protects anoikis. B. Caspase-3 activation in EGFP- & EGFP-Survivin-expressing cells after serum starvation under attached or detached culture conditions. Experimental protocol was illustrated in Figure S1. Transfection frequencies checked by using fluorescence microscopy & confirmed to be 80–90%. Cells kept in serum-free medium for 24–72 h, harvested, & lysed in Laemmli SDS-sample buffer for immunoblot analysis with anti-GFP, anti-Survivin, anti-activated caspase-3 antibody, anti-LC3B, & anti-alpha -tubulin.Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0055710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Representative Western blots showing the IAP levels in EV derived from individual European American (EA) (N1-N5) & (N7-N9), *N6 & African American (AA) (N1-N9) patients with prostate cancer (PCa).Specific antibodies against Survivin, XIAP, cIAP-1, cIAP-2, & Lamp1 were used for the Western blotting analysis of total exosomal proteins. The blots from both patient groups were processed under identical conditions; Lamp 1 was used as loading control. (*N6, Hispanic.) (Both blots were done side by side in the same gel running & transferring apparatus, blocking, washing buffers, & antibody incubations were done in the same time, in the same incubating trays under the identical exposure to keep the consistencies.) Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0183122), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Survivin Antibody - BSA Free [NB500-201] - CCAR2 binds Hsp60 & survivin. Interaction between CCAR2 & survivin was examined in SH-SY5Y or HEK293 cells by co-immunoprecipitation with either an anti-CCAR2 or an anti-survivin antibody, followed by western blotting. (A) Interaction between CCAR2 & survivin in whole cell lysates from SH-SY5Y cells was examined. (B) Interaction between CCAR2, survivin, & Hsp60 in cytosolic & mitochondrial fractions isolated from HEK293 cells was examined. (C) SH-SY5Y cells were depleted of Hsp60 & the interaction between CCAR2 & survivin was examined. siU, universal siRNA; siH, Hsp60 siRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30609639), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Induction of cell death & suppression of survivin after L-OHP depends on p53(A) HCT116 wild typeand p53-/- cells were treated with 5 μM L-OHP or 10 μM CPT-11 for 24 hours. Protein levels of survivin, p53 & p21 were detected by Western blot analysis; vinculin serves as loading control. (B) Quantitative real-time PCR was performed to quantify BIRC5 mRNA levels in HCT116 wild type & p53-deficient cells after 24 hours treatment (** p < 0.01, n = 3). (C) Flow cytometric analysis of DNA content was done in HCT116 wild type & p53-/- cells after 24 hours treatment with L-OHP (n = 4). (D) SubG1-populations were detected in both cell lines after 48 hours treatment (*** p < 0.001, n = 4). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29963241), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Detection of BCL2, Bcl-xL, XIAP & survivin protein content by western blotting 48 h after transfection with a total of 40 nM siRNA in EJ28 bladder cancer cells. Beta-actin was used for loading control. Image collected & cropped by CiteAb from the following publication (//www.spandidos-publications.com/10.3892/ijo.2012.1549), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - PARP6 inhibits cell growth & colony formationA. A schematic illustration of the human PARP6 protein. PARP6 consists of 630 amino acid residues & has a PARP catalytic domain in C-terminal region. delta C-PARP6 lacks the PARP catalytic domain. B. Cell proliferation after FL-PARP6 & delta C-PARP6 overexpression in SW480 cells was measured by MTT assays. After transfection with the p-EGFP-empty, p-EGFP-FL-PARP6 or p-EGFP-delta C-PARP6 plasmids, transfectant cells (1500 cells / well) were replated in 96-well plates. The MTT assay was performed to test the cell viability at 24 h, 48 h & 72 h. Values indicate mean ± SD (n=6). C. Colony formation aof FL-PARP6 & delta C-PARP6 transfectant SW480 cells. Cells were plated in 6 cm dishes at a density of 500 cells per dish. After 2 weeks, number of colonies were counted. The data represent the means ± S.D. of three independent experiments **P < 0.01. D. Expression of cell cycle related proteins including Survivin, Cyclin B, Aurora-B, p21 & p27 in empty vector, FL-PARP6 & delta C-PARP6 transfectant SW480 cells was examined by Western blot analysis. beta -actin expression was used as a loading control. E. Expression of apoptosis related proteins including cleaved Caspase-3, Caspase-3, Bax, Bcl-2 & Bcl-XL in empty vector, FL-PARP6 & delta C-PARP6 transfectant SW480 cells was examined by Western blot analysis. beta -actin expression was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26934315), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Screening of epigenetic drugs for upregulation of miRNAs & downregulation of ZEB1Heat map showing the relative expression levels after drug treatment for 48 h in Panc1. Values measured by qRT–PCR were depicted with the software GENE-E. Only mocetinostat upregulated the miRNAs & downregulated ZEB1.Relative expression of indicated genes in Panc1 measured by qRT–PCR after treatment with different HDAC inhibitors. Note the downregulation of ZEB1 & upregulation of miR-203, miR-200, & E-cadherin by mocetinostat. n = 3, mean ± SEM; unpaired Student's t-test. For significance, see Supplementary Table S1.Immunoblot & immunofluorescence showing that mocetinostat treatment (1 μM, 48 h) reduced ZEB1 expression & induced E-cadherin in Panc1. Expression of histone deacetylases was not altered by mocetinostat, but histone acetylation was induced. Scale bar 10 μm.Chromatin immunoprecipitation analysis validated mocetinostat-induced (1 μM, 48 h) enrichment of the active histone marks H3ac, H4ac, H3K9ac, & H3K4me3 at ZEB1 target gene loci in Panc1. n = 3, mean ± SEM; unpaired Student's t-test.Mocetinostat treatment reduced expression of the anti-apoptotic miR-203 target survivin & sphere-forming capacity in Panc1 when pre-treated with mocetinostat for 48 h. n = 3, mean ± SEM; Mann–Whitney U-test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25872941), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Survivin is highly enriched in exosomes from PTX-treated cancer cells. (A) Western blot analysis using Survivin, flotillin, I kappa B alpha , & CD-63 antibodies was performed on lysates of MDAMB231 cells treated with either DMSO or PTX (lanes labeled WCL), as well as the exosomes (lanes labeled Exos) & MVs (lanes labeled MVs) generated by the cells. (B) The relative amounts of Survivin detected in exosomes generated by DMSO- & PTX-treated MDAMB231 cells. (C) Western blot analysis using Survivin & actin antibodies was performed on lysates of MDAMB231 cells treated with PTX for increasing lengths of time. (D) Immunofluorescence using a Survivin antibody was performed on MDAMB231 cells treated with either DMSO or PTX (top images). The cells were also stained with DAPI to label nuclei (bottom images). Arrows indicate areas where Survivin is detected as puncta in the cytosol of cells treated with PTX. Scale bar = 5 µm. (E) Western blot analysis was performed using a Survivin antibody on lysates of MDAMB231 cells, U87 glioblastoma cells, & SKBR3 cells that had been treated with DMSO or PTX (lanes labeled WCL), & on the exosomes these cells generated (lanes labeled Exos). (F) Western blot analysis was performed on lysates of exosomes from MDAMB231 cells that had been treated with the indicated chemotherapeutic agents & inhibitors. The experiments in B were performed a minimum of three separate times, with each experiment yielding similar results. Student t-tests were performed. *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/8/12/111), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - NPC-derived EBNA1 is compromised for interaction with SurvivinA. LC-MS analysis of proteins associated with FLAG-EBNA1 (F-EBNA1) from B95-8 or with the NPC DNA binding domain. Unique peptides & spectral counts are shown for USP7, BIRC5 (Survivin), & Tankyrase 1 (TNKS). B. Western blot analysis of FLAG IP from HeLa cells with stable expression of FLAG-vector, F-EBNA1 (B95-8) or F-EBNA1 (NPC) probed with antibody to USP7, Tankyrase 1, Survivin, or FLAG. C. Extracts from stable HeLa cells shown in panel B, were subject to IP with antibody to Survivin probed with antibody to FLAG or Survivin (top panel). Extracts from stable HeLa cells shown in panel B were subject to IP with antibody to Tankyrase 1 probed with antibody to FLAG or Tankyrase1 (lower panel). D. MutuI cell (top panels) or C666-1 cell (lower panels) extracts were subject to IP with antibody to EBNA1 or control IgG, & then assayed by Western blot with antibody to Survivin or EBNA1, as indicated. E. Same as in panel D, except IP with Suvivin or control IgG. F. In situ Proximity Ligation Assay (in situ PLA) is shown for interphase cells from either MUTU-I or C666-1 using mouse anti-EBNA1 & rabbit anti-Survivin. G. Quantification of in situ PLA for n > 100. ** p-value < .01 using student t-test. H. PFGE analysis of EBV episomes & linears at 7 days post-transduction with lentivirus shRNA for shControl or shSurvivin in MUTU I or RAJI cell lines. Human alpha satellite DNA is used for DNA loading control Western blot of Survivin & Actin are shown below for each cell at 7 days post-transduction. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28077791), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Anti-tumor effects & functional evidence of sCA-survivin-siRNA in HCT116 & HT29 solid tumor models.(A) For western blot analysis, HCT116 cells were seeded into 6-well plates & transfected with 100 pmol/well of either control or survivin siRNA by Lp (Lipofectamine 2000) or sCA. Actin blots served as loading controls. (B) For proliferation assays, cells were uniformly seeded into 96-well plates (1 × 104 cells/well), & 5 pmol/well siRNA was used. Cell viability was examined at 48 & 72 h by WST-8 assay. Data represent mean ± SEM. *P = 0.0294, **P = 0.0304 (n = 4, Wilcoxon rank test). (C) In vivo tumor growth. Each vehicle, carrying 15 μg of control siRNA or survivin siRNA, was administered by intravenous injection to mice with HCT116 tumors. Data represent the mean ± SEM (n = 10 tumors, Wilcoxon rank test). (D) Immunostaining of survivin in the tumor tissues on day 19. Scale bar, 50 μm. (E) In vivo live imaging of sCA-siRNA (6-FAM labeled) in HT29 tumor by multiphoton microscopy at 90 min. (F) Fluorescent detection of naked-siRNA (6-FAM labeled) or sCA-siRNA (6-FAM labeled) in the HT29 tumor at 4 h. Green: 6-FAM labeled siRNA; Red: microvasculature; Blue: DAPI stained nuclei. Scale bar, 50 μm. (G) Mice were administered with 40 μg of naked-survivin-siRNA or sCA-survivin-siRNA on days 0, 1, & 2. Tumors were removed on day 3, & western blot analysis for survivin was performed. (H) Many tumor cells treated with sCA-survivin-siRNA (6-FAM labeled) had condensed nuclei & were positive for TUNEL assay. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25738937), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Survivin regulates autophagic flux. (A) U2OS cells stably expressing survivinWTGFP or GFP alone were treated with RAP (200 nM) & CQ (120 µM) for 2 h, then lysed & immunoblotted with anti-LC3, anti-survivin & anti-tubulin antibodies. Immunoblot shown is representative of four independent experiments. (B) ImageJ quantitation of LC3II signals in (A), normalised against tubulin control & expressed as band intensity relative to untreated GFP cells. (C) Data from (B) expressed as a percentage increase in LC3II between CQ treated & untreated cells to indicate autophagic flux. (D) The above cell lines were treated with CQ (50 µM) for 8 h & p62 levels assessed by immunoblotting at 2 h intervals. Blot shown is representative of three independent experiments. (E) ImageJ quantitation of p62 signals in (D) normalised against tubulin & expressed as band intensity relative to untreated GFP cells. Error bars indicate s.e.m., N=3. *P<0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30348810), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Cdh1 depletion induces early DNA replication. (A) Fzr1+/+ & Fzr1−/− MEFs were synchronized at G1-S phase by double thymidine block (DTB, see diagram). After release from G1-S arrest, cells were collected at the indicated times. Cell extracts were immunoblotted for the indicated proteins. M phase is evaluated by detection of FoxM1 p-T600. beta -actin is shown as a loading control. Asterisk shows the position of Aurora A p-T288. (B) Subcellular colocalization of Aurora B & histone H3 p-S10 in Fzr1+/+ & Fzr1−/− MEFs. Cells were fixed & stained using anti-H3 p-S10 (green) & anti-Aurora B (red) antibodies. Nuclei of the cells were stained using DAPI (blue). Arrowheads show H3 p-S10 in mitotic cells, & arrows show H3 p-S10 in interphase cells. Scale bar: 20 µm. (C) Fzr1+/+ & Fzr1−/− MEFs were fixed & stained using anti-H3 p-S10 (green) & anti-cyclin A (red) antibodies. Nuclei of the cells were stained with DAPI (blue). Cyclin A expression was used as a marker of G2 cells. Scale bar: 10 µm. (D) Fzr1−/− MEFs were treated with the Aurora B specific inhibitor barasertib (10 nM) for 24 h. Cells were fixed & stained using an anti-H3 p-S10 antibody (green). Nuclei of the cells were stained using DAPI (blue). Scale bar: 10 µm. (E) Fzr1+/+ & Fzr1−/− MEFs were serum starved for 72 h, forced to enter the cell cycle after stimulation with 15% FBS & pulsed with EdU. The percentage of EdU-positive cells was scored at different time points. Three independent experiments were used to calculate the mean±s.d. Blots & images in A–D are representative of at least two experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32934012), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Borealin is degraded at G1 phase via APC/CCdh1-mediated polyubiquitylation. (A) 293T cells co-transfected w/ FLAG–Borealin & empty vector (EV), HA–Cdh1, HA–Cdc20, Myc–CUL1, Myc–CUL2, Myc–CUL3, Myc–CUL4, or Myc–CUL5. Then, cells treated w/ 10 µM MG132 for 5 h. Cell extracts immunoprecipitated (IP) w/ an affinity-purified pAb against HA or Myc, & analyzed by immunoblotting as indicated. beta -actin expression used as a loading control. WCE: whole cell extracts. Asterisk indicates non-specific bands. (B) GST-tagged wild-type (WT) borealin bound to glutathione sepharose 4B resin incubated w/ in vitro translated (IVT) Cdh1 for 2 h at 4C. After washing, these proteins eluted & analyzed by immunoblotting using anti-Cdh1 antibody. GST-tagged borealin & GST visualized by Coomassie Blue (CBB) staining. IB, immunoblot. (C) HA-tagged Cdh1 (0, 0.5, 1.0, 1.5 & 2.0 μg) co-transfected w/ FLAG-tagged borealin (0.5 μg) in 293T cells. Cells then collected & lysed for immunoblotting as indicated. beta -actin expression used as a loading control. (D) HeLa cells transfected w/ control or Cdh1 siRNA & synchronized at prometaphase arrest using nocodazole (Noc). After mitotic shake-off, cells released & collected at indicated times. Cell extracts immunoblotted for the indicated proteins. beta -actin expression used as a loading control. (E) An in vivo ubiquitylation assay performed. FLAG-tagged borealin & HA-tagged ubiquitin co-transfected w/ either control, Cdc20 or Cdh1 siRNA in 293T cells. Cell extracts immunoprecipitated using an anti-FLAG antibody, & the precipitates blotted w/ anti-HA & anti-FLAG antibodies. Expression of endogenous Cdc20 & Cdh1 also examined. beta -actin expression used as a loading control. Blots shown representative of two experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32934012), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Borealin is degraded at G1 phase via the ubiquitin–proteasome pathway. (A) HeLa cells were released from a prometaphase arrest with nocodazole (Noc) & collected at the indicated times (left panel). In addition, HeLa cells were synchronized at the G1-S border using a double thymidine block (DTB). After release, cells were collected at the indicated time points (right panel). Cells were then lysed for immunoblotting as indicated. beta -actin expression was used as a loading control. As; asynchronous. (B) The schematic graph shows protein expression level of borealin & APC/C activity during cell-cycle progression, based on the results shown in A. (C) HeLa cells were synchronized in M phase by mitotic shake-off with nocodazole (M). After 2 h release from mitotic arrest (G1), cells were treated with or without 10 μM MG132 or 10 μM lactacystin for 5 h. Asynchronous cells (As) were also treated with or without 10 μM MG132 or 10 μM lactacystin for 5 h. Cells were then collected & lysed for immunoblotting as indicated. beta -actin expression was used as a loading control. Blots shown in A & C are representative of two experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32934012), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Pro-apoptotic activity of SAB-13 & SAB-14. (A–C,E,F), FACS analysis of the cells after 48 h treatment. Analysis of apoptosis induction in 22Rv1 (A) & PC-3 cells (B) using Annexin-V-FITC/propidium iodide (PI) double staining. PC-3 cells were pre-treated with 100 µM of pan-caspase inhibitor z-VAD(OMe)-fmk (zVAD) for 1 h & then treated with indicated concentrations of the drugs for 48 h (B). Viable cells (Annexin-V-FITC(–)/PI(–), LL quadrant) or early apoptotic cells (Annexin-V-FITC(+)/PI(–), LR quadrant) were quantified using the Cell Quest Pro software (C) (mean ± SEM; n = 3; * p < 0.05, Student′s t-test). (D) Western blotting analysis of the protein expression in 22Rv1 cells after 48 h of treatment. beta -actin was used as a loading control (mean ± SEM; n = 3; one-way ANOVA test). Anisomycin (Aniso; treatment with 10 µM for 48 h) was used as a positive control. (E,F) Cell cycle analysis of 22Rv1 cells using PI staining, apoptotic cells were detected as sub-G1 population (E) (mean ± SEM; n = 3; * p < 0.05, one-way ANOVA test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32403427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Survivin Antibody - BSA Free [NB500-201] - Protein expression pattern of Survivin, XIAP & XAF1 in HCC tissues & non-neoplastic liver parenchyma. IAP members immunoreactivity was estimated by tissue microarray in a subset of HCC patients (n = 40). A-D, Representative Survivin cytoplasmatic immunostaining in a tumor core (A), in a tumor proximal to cirrhosis (C, N: cirrhosis, K: HCC), & in adjacent & long-distance non-neoplastic parenchyma (B & D, respectively). XIAP marked (score 12) & moderate (score 8) immunoreactivity is shown for HCC (E & I, respectively) as well as for cirrhosis (F & L, respectively). G & H, Nuclear XAF1 staining is shown for tumor & non-neoplastic liver whereas XAF1 cytoplasmatic expression in HCC & cirrhosis is shown in panels M & N, respectively. Original magnification ×50 & ×250, for tissue cores & insets, respectively. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-9-125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Survivin Antibody - BSA Free [NB500-201] - Survivin affects cellular susceptibility to chemotherapeutic drugs(A) siRNA-mediated knockdown of survivin was performed in HCT116 cells for 24 hours (scrambled siRNA (siCtrl) transfection serves as control). Thereafter, cells were treated with 10 μM CPT-11 for 24 hours. Western blot analysis detected protein levels of survivin, p53, as well as cleavage products of caspase-3 & PARP1; vinculin serves as loading control. (B) HCT116 cells were transfected with 0.1 μg & 0.25 μg survivin-MYC plasmid for 24 hours & were treated 5 μM L-OHP for additional 24 & 48 hours. Western blot analysis detected MYC-tag, cleavage of caspase-3 & PARP1; vinculin serves as loading control (n = 2). (C) HCT116 cells were treated with 3 μM ETP-46464 for 1 hour, after which 10 μM CPT-11 were added for additional 24 hours. Western blot was carried out as indicated, with vinculin as loading control (n = 2). (D) HCT116 cells were treated as described in C, but for 48 hours total incubation time. Cells were harvested & analyzed for the occurrence of cells in the subG1 fraction (n=3). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29963241), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Deficiency of CCAR2 or Hsp60 reduces expression of survivin mRNA. SH-SY5Y (A–E) or BE(2)-M17 cells (E) were transfected with Universal (siU), CCAR2 (siC), or Hsp60 (siH) siRNA. (A,B) Forty-eight hours later, the level of survivin protein was examined by western blotting. Cells deficient in CCAR2 & Hsp60 were treated with 25 μM MG132 (A) or 100 μM chloroquine (B) 4 h or 24 h prior to cell lysis, respectively. (C,D) The level of survivin mRNA in each group of siRNA-transfected cells was measured by RT-PCR. (C) Levels were normalized against beta -actin & quantified using ImageJ software. The relative level of survivin mRNA is expressed as the mean ± standard error of the mean (SEM) (n = 3). Asterisks (*) denote statistically significant differences (p < 0.05, one-way ANOVA). (D) Two different siRNAs targeting CCAR2 & Hsp60 were used to knock down their expressions. (E) The level of each protein was examined by western blotting. The relative level is expressed as the mean ± standard error of the mean (SEM) (n = 3). Indicators (*, #) denote statistically significant differences from the corresponding control cells (p < 0.05, one-way ANOVA). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30609639), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Correlation between PARP6 & Survivin expression in CRCA. Expression of PAPR6 & Survivin in 238 CRC cases was examined by immunohistochemistry. Representative images of PARP6, Ki-67 & Survivin in normal colonic mucosa, well differentiated adenocarcinoma & poorly differentiated adenocarcinoma cases. B. Correlation between PARP6 & proliferation marker, Ki-67 in CRC cases by immunohistochemistry. Graph shows Ki-67 index in CRC cases with low or high expression of PARP6. C. Correlation between PARP6 & Survivin in CRC cases by immunohistochemistry. Graph shows Ki-67 index in CRC cases with low or high expression of Survivin. D. The expression of PAPR6 & Survivin were determined in 4 CRC tissues & normal adjacent colorectal tissues by Western blot analysis. beta -actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26934315), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Proximity Ligation Assay: Survivin Antibody - BSA Free [NB500-201] - NPC-derived EBNA1 is compromised for interaction with SurvivinA. LC-MS analysis of proteins associated with FLAG-EBNA1 (F-EBNA1) from B95-8 or with the NPC DNA binding domain. Unique peptides & spectral counts are shown for USP7, BIRC5 (Survivin), & Tankyrase 1 (TNKS). B. Western blot analysis of FLAG IP from HeLa cells with stable expression of FLAG-vector, F-EBNA1 (B95-8) or F-EBNA1 (NPC) probed with antibody to USP7, Tankyrase 1, Survivin, or FLAG. C. Extracts from stable HeLa cells shown in panel B, were subject to IP with antibody to Survivin probed with antibody to FLAG or Survivin (top panel). Extracts from stable HeLa cells shown in panel B were subject to IP with antibody to Tankyrase 1 probed with antibody to FLAG or Tankyrase1 (lower panel). D. MutuI cell (top panels) or C666-1 cell (lower panels) extracts were subject to IP with antibody to EBNA1 or control IgG, & then assayed by Western blot with antibody to Survivin or EBNA1, as indicated. E. Same as in panel D, except IP with Suvivin or control IgG. F. In situ Proximity Ligation Assay (in situ PLA) is shown for interphase cells from either MUTU-I or C666-1 using mouse anti-EBNA1 & rabbit anti-Survivin. G. Quantification of in situ PLA for n > 100. ** p-value < .01 using student t-test. H. PFGE analysis of EBV episomes & linears at 7 days post-transduction with lentivirus shRNA for shControl or shSurvivin in MUTU I or RAJI cell lines. Human alpha satellite DNA is used for DNA loading control Western blot of Survivin & Actin are shown below for each cell at 7 days post-transduction. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28077791), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - The p53-p21 axis facilitates downregulation of survivin via cell cycle(A) HCT116 wild typeand p21-deficient (p21-/-) cells were treated with 5 μM L-OHP for 24 hours. Whole cell lysates were analyzed with antibodies against p53, p21, & survivin; vinculin serves as loading control. (B) Cell cycle distribution was analyzed after 24 hours treatment by flow cytometry analysis (n = 3). (C) To induce p21, RKO p21ind cells were treated with 3 nM Muristerone A for 24 hours & tested for the levels of p21 & survivin; vinculin, loading control. (D)BIRC5 mRNA levels were analyzed by quantitative real-time PCR after 24 hours treatment with MurA in RKO p21ind cells (n = 3). (E) Cell cycle distribution was measured by flow cytometry analyses of cellular DNA content (n = 3). (F) Scheme summarizing the supposed mechanisms of survivin regulation after L-OHP & CPT-11 treatment. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29963241), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Apoptosis & survival signaling after L-OHP & CPT-11(A) Western blot analysis using antibodies against p53 & pro-apoptotic BAX & PIG-3 after treatment with 5 μM L-OHP or 10 μM CPT-11. (B) Immunodetection of NF-kappa B p65, RELB & anti-apoptotic survivin, XIAP, BCL-XL & MCL1; vinculin serves as loading control. (C) Effects of increasing doses L-OHP & CPT-11 on caspase-3 & PARP1 cleavage after 24 hours treatment; alpha -tubulin serves as loading control. (D) Cells were treated with a combination of L-OHP & the caspase-inhibitor Z-VAD-FMK (50 μM). Immunodetection of survivin, p53 & full-length caspase-3 was conducted. Detection of apoptosis was determined by cleavage products of caspase-3 & PARP1; beta -actin serves as loading control. Please note: Figure 4A & 4B, as well as Supplementary Figure 2A show signals acquired by different detection methods, but originate from the same Western blots. This is due to a switch in the immunoblot chemiluminescence detection system from X-ray films (darker background) to a CCD camera system (Fusion Solo S, Vilber Lourmat; lighter background). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29963241), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Survivin Antibody - BSA Free [NB500-201] - Survivin is essential for the salinomycin-induced apoptosis in UM cells. a UM cells were treated with salinomycin for 24 h & the protein levels of apoptosis-related proteins were detected by Western blot. b & c UM cells were transduced with lentiviral pTSB-Survivin cDNA (b), Survivin-shRNA constructs (c), or their corresponding empty vectors, & then incubated in the presence of puromycin (1 μg/mL) for 5 days to reach stable clones. Such survivin-manipulated cells were then exposed to salinomycin for 24 h, & subjected to trypan blue exclusion assay (left) & Western blot assay (right). Data represent mean ± SD. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001, Student’s t test. d qRT-PCR analysis of BIRC5 mRNA level was done in the 92.1 & Mel270 cells treated with salinomycin for 24 h. **, P < 0.01; ***, P < 0.001, one-way ANOVA, post hoc comparisons, Tukey’s test Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31718679), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Survivin Antibody - BSA Free [NB500-201] - Anti-tumor effects & functional evidence of sCA-survivin-siRNA in HCT116 & HT29 solid tumor models.(A) For western blot analysis, HCT116 cells were seeded into 6-well plates & transfected with 100 pmol/well of either control or survivin siRNA by Lp (Lipofectamine 2000) or sCA. Actin blots served as loading controls. (B) For proliferation assays, cells were uniformly seeded into 96-well plates (1 × 104 cells/well), & 5 pmol/well siRNA was used. Cell viability was examined at 48 & 72 h by WST-8 assay. Data represent mean ± SEM. *P = 0.0294, **P = 0.0304 (n = 4, Wilcoxon rank test). (C) In vivo tumor growth. Each vehicle, carrying 15 μg of control siRNA or survivin siRNA, was administered by intravenous injection to mice with HCT116 tumors. Data represent the mean ± SEM (n = 10 tumors, Wilcoxon rank test). (D) Immunostaining of survivin in the tumor tissues on day 19. Scale bar, 50 μm. (E) In vivo live imaging of sCA-siRNA (6-FAM labeled) in HT29 tumor by multiphoton microscopy at 90 min. (F) Fluorescent detection of naked-siRNA (6-FAM labeled) or sCA-siRNA (6-FAM labeled) in the HT29 tumor at 4 h. Green: 6-FAM labeled siRNA; Red: microvasculature; Blue: DAPI stained nuclei. Scale bar, 50 μm. (G) Mice were administered with 40 μg of naked-survivin-siRNA or sCA-survivin-siRNA on days 0, 1, & 2. Tumors were removed on day 3, & western blot analysis for survivin was performed. (H) Many tumor cells treated with sCA-survivin-siRNA (6-FAM labeled) had condensed nuclei & were positive for TUNEL assay. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25738937), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Survivin was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Rabbit anti-Survivin Antigen Affinity Purified Polyclonal Antibody (Catalog # NB500-201) at 2.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells in various stages of mitosis were imaged using a 100X objective and digitally deconvolved.

Survivin was detected in immersion fixed NIH-3T3 Mouse fibroblast cell line using Rabbit anti-Survivin Antigen Affinity Purified Polyclonal Antibody (Catalog # NB500-201) at 2.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rt, Ca, Fe, Gp, Ha

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Fe, Gp, Ha

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-201AF350
Alexa Fluor 405	NB500-201AF405
Alexa Fluor 488	NB500-201AF488
Alexa Fluor 532	NB500-201AF532
Alexa Fluor 594	NB500-201AF594
Alexa Fluor 647	NB500-201AF647
Alexa Fluor 700	NB500-201AF700
Alexa Fluor 750	NB500-201AF750
Alexa Fluor Plus 405	NB500-201AFP405
Alexa Fluor Plus 488	NB500-201AFP488
Alexa Fluor Plus 555	NB500-201AFP555
Alexa Fluor Plus 594	NB500-201AFP594
Alexa Fluor Plus 647	NB500-201AFP647
Alexa Fluor Plus 680	NB500-201AFP680
Allophycocyanin	NB500-201APC
Biotin	NB500-201B-0.1ml
CoraFluor 1	NB500-201CL1
DyLight 350	NB500-201UV
DyLight 405	NB500-201V-0.1ml
DyLight 488	NB500-201G-0.1ml
DyLight 550	NB500-201R-0.1ml
DyLight 594	NB500-201DL594
DyLight 650	NB500-201C-0.1ml
DyLight 680	NB500-201FR
DyLight 755	NB500-201IR
FITC	NB500-201F-0.1ml
HRP	NB500-201H-0.1ml
Janelia Fluor 525	NB500-201JF525
Janelia Fluor 549	NB500-201JF549
Janelia Fluor 646	NB500-201JF646
Janelia Fluor 669	NB500-201JF669
PE	NB500-201PE
PerCP	NB500-201PCP
mFluor Violet 450 SE	NB500-201MFV450
mFluor Violet 500 SE	NB500-201MFV500
mFluor Violet 610 SE	NB500-201MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB500-201SS

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NBP2-00776

CDK2 Antibody (OTI2D9)

(1 Review)

0.1 ml

NBP2-00776

Immunohistochemistry-Paraffin: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.

Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cdk2 antibody, and then analyzed by flow cytometry.

Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk2.

Immunocytochemistry/Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Immunofluorescent staining of HeLa cells using anti-Cdk2 mouse monoclonal antibody.

Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - Analysis of Hela cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - Analysis of Jurkat cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70392AF350
Alexa Fluor 405	NBP2-70392AF405
Alexa Fluor 488	NBP2-70392AF488
Alexa Fluor 532	NBP2-70392AF532
Alexa Fluor 594	NBP2-70392AF594
Alexa Fluor 647	NBP2-70392AF647
Alexa Fluor 700	NBP2-70392AF700
Alexa Fluor 750	NBP2-70392AF750
Alexa Fluor Plus 405	NBP2-70392AFP405
Alexa Fluor Plus 488	NBP2-70392AFP488
Alexa Fluor Plus 555	NBP2-70392AFP555
Alexa Fluor Plus 594	NBP2-70392AFP594
Alexa Fluor Plus 647	NBP2-70392AFP647
Alexa Fluor Plus 680	NBP2-70392AFP680
Allophycocyanin	NBP2-70392APC
Biotin	NBP2-70392B
CoraFluor 1	NBP2-70392CL1
DyLight 350	NBP2-70392UV
DyLight 405	NBP2-70392V
DyLight 488	NBP2-70392G
DyLight 550	NBP2-70392R
DyLight 594	NBP2-70392DL594
DyLight 650	NBP2-70392C
DyLight 680	NBP2-70392FR
DyLight 755	NBP2-70392IR
FITC	NBP2-70392F
HRP	NBP2-70392H
Janelia Fluor 525	NBP2-70392JF525
Janelia Fluor 549	NBP2-70392JF549
Janelia Fluor 646	NBP2-70392JF646
Janelia Fluor 669	NBP2-70392JF669
PE	NBP2-70392PE
PerCP	NBP2-70392PCP
mFluor Violet 450 SE	NBP2-70392MFV450
mFluor Violet 500 SE	NBP2-70392MFV500
mFluor Violet 610 SE	NBP2-70392MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70392

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NBP1-87262

Cdk6 Antibody - BSA Free

(3 Publications)

0.1 ml

NBP1-87262

Immunocytochemistry/Immunofluorescence: Cdk6 Antibody [NBP1-87262] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

<b>Orthogonal Strategies Validation. </b>Western Blot: Cdk6 Antibody [NBP1-87262] - Analysis in human cell lines U2OS and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in germinal center cells.

Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-87262-25ul

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NBP1-31308

CDK4 Antibody - BSA Free

(7 Publications)

100 ul

NBP1-31308

Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Western Blot: CDK4 Antibody [NBP1-31308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.

Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: CDK4 Antibody [NBP1-31308] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Bv, Po, RM, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Po, RM, Sh

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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AF4654

CDK2 Antibody [Unconjugated]

(4 Publications)

100 μg

AF4654

HAF017). For additional reference, recombinant human CDK2, CDK4, and CDK6 (5 ng/lane) were included. A specific band for CDK2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). For additional reference, recombinant human CDK2, CDK4, and CDK6 (5 ng/lane) were included. A specific band for CDK2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). For additional reference, recombinant human CDK2, CDK4, and CDK6 (5 ng/lane) were included. A specific band for CDK2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for CDK2 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for CDK2 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CDK2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

CDK2 was detected in immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line using Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cancer cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CDK2 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cancer cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

LPS suppressed EPCs cell viability via inducing cell pyroptosis. (a, b) NLRP3 was silenced in EPCs cells. (c) MTT assay was used to examine cell viability. (d) The expression status of Cyclin D1 and CDK2 were determined by Western blot analysis. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for CDK2 at approximately 38 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4654U-100UG
Alexa Fluor 405	AF4654V-100UG
Alexa Fluor 488	AF4654G-100UG
Alexa Fluor 532	AF4654X-100UG
Alexa Fluor 594	AF4654T-100UG
Alexa Fluor 647	AF4654R-100UG
Alexa Fluor 700	AF4654N-100UG
Alexa Fluor 750	AF4654S-100UG
Alexa Fluor Plus 405	AF4654AFP405-100UG
Alexa Fluor Plus 488	AF4654AFP488-100UG
Alexa Fluor Plus 555	AF4654AFP555-100UG
Alexa Fluor Plus 594	AF4654AFP594-100UG
Alexa Fluor Plus 647	AF4654AFP647-100UG
Alexa Fluor Plus 680	AF4654AFP680-100UG
Allophycocyanin	AF4654A
Biotin	AF4654B
CoraFluor 1	AF4654CL1
DyLight 350	AF4654D
DyLight 405	AF4654E
DyLight 488	AF4654K
DyLight 550	AF4654L
DyLight 594	AF4654M
DyLight 650	AF4654W
DyLight 680	AF4654Y
DyLight 755	AF4654Z
FITC	AF4654F
HRP	AF4654H
Janelia Fluor 525	AF4654JF525
Janelia Fluor 549	AF4654I
Janelia Fluor 646	AF4654J
Janelia Fluor 669	AF4654JF669
PE	AF4654P
PerCP	AF4654C
mFluor Violet 450 SE	AF4654MFV450
mFluor Violet 500 SE	AF4654MFV500
mFluor Violet 610 SE	AF4654MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4654-SP

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NB120-6315

CDK4 Antibody (DCS-31)

(2 Publications)

0.25 ml

NB120-6315

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-37507

CDK2 Antibody (1A6) - BSA Free

(1 Review)

0.1 ml

NBP2-37507

Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of colon cancer tissues using CDK2 mouse mAb with DAB staining.

Flow Cytometry: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of Jurkat cells using CDK2 mouse mAb (green) and negative control (red).

Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mAb against human CDK2 (AA: 197-295) recombinant protein. (Expected MW is 36.8 kDa)

Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of cervical cancer tissues using CDK2 mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of CDK2 in colon cancer tissue using anti-CDK2 antibody. Image submitted by a verified customer review.

ELISA: CDK2 Antibody (1A6) [NBP2-37507] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37507-0.025ml

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NBP3-15345

Cdk9 Antibody (1C4E3)

100 ul

NBP3-15345

Immunohistochemistry-Paraffin: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunohistochemistry of paraffin-embedded mouse brain using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Chromatin Immunoprecipitation Sequencing: Cdk9 Antibody (1C4E3) [NBP3-15345] - Chromatin immunoprecipitations were performed with cross-linked chromatin from DLD-1 cells and Cdk9 Rabbit mAb (NBP3-15345). The ChIP sequencing results indicate the enrichment pattern of Cdk9 in selected genomic region and representative gene loci (e.g. RAC1, GAPDH and ACTB), as shown in figure.

Immunocytochemistry/Immunofluorescence: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunofluorescence analysis of HeLa cells using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunohistochemistry of paraffin-embedded rat ovary using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunohistochemistry of paraffin-embedded human esophageal using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Cdk9 antibody (NBP3-15345). Western blot was performed from the immunoprecipitate using Cdk9 antibody (NBP3-15345) at a dilition of 1:1000.

Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

<b>Genetic Strategies Validation. </b>Western Blot: Cdk9 Antibody (1C4E3) [Cdk9] - Western blot analysis of lysates from wild type(WT) and Cdk9 knockout (KO) 293T cells, using [KO Validated] Cdk9 Rabbit mAb at 1:500 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 1s.

Immunocytochemistry/ Immunofluorescence: Cdk9 Antibody (1C4E3) [Cdk9] - Confocal imaging of U-2 OS cells using [KO Validated] Cdk9 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Immunohistochemistry: Cdk9 Antibody (1C4E3) [Cdk9] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KO Validated] Cdk9 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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H00001021-M01

Cdk6 Antibody (8H4) - Azide and BSA Free

(4 Publications)

0.1 mg

H00001021-M01

Immunohistochemistry-Paraffin: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of monoclonal antibody to CDK6 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.

Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - CDK6 monoclonal antibody (M01), clone 8H4. Analysis of CDK6 expression in PC-12.

Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - CDK6 monoclonal antibody (M01), clone 8H4 Analysis of CDK6 expression in Jurkat.

Proximity Ligation Assay: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of protein-protein interactions between CDK2 and CDK6. HeLa cells were stained with anti-CDK2 rabbit purified polyclonal 1:1200 and anti-CDK6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

ELISA: Cdk6 Antibody (8H4) [H00001021-M01] - Detection limit for recombinant GST tagged CDK6 is 0.3 ng/ml as a capture antibody.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-15344

CDK4 Antibody (6N3M6)

100 ul

NBP3-15344

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat ovary using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western Blot: CDK4 Antibody (6N3M6) [CDK4] - Western blot analysis of various lysates, using CDK4 Rabbit mAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 10s.

Immunohistochemistry: CDK4 Antibody (6N3M6) [CDK4] - Immunohistochemistry analysis of paraffin-embedded Human prostate tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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AF5254

CDK4 Antibody [Unconjugated]

(2 Publications)

100 μg

AF5254

HAF017). A specific band was detected for CDK4 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CDK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for CDK4 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CDK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CDK4 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

CDK4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse CDK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5254) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CDK4 was detected in immersion fixed RAW264.7 cells using Goat Anti-Human/Mouse CDK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5254) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cells, and MCF‑7 human breast cancer cells, loaded at 0.2 mg/mL. A specific band was detected for CDK4 at approximately 38 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse CDK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5254). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF5254U-100UG
Alexa Fluor 405	AF5254V-100UG
Alexa Fluor 488	AF5254G-100UG
Alexa Fluor 532	AF5254X-100UG
Alexa Fluor 594	AF5254T-100UG
Alexa Fluor 647	AF5254R-100UG
Alexa Fluor 700	AF5254N-100UG
Alexa Fluor 750	AF5254S-100UG
Alexa Fluor Plus 405	AF5254AFP405-100UG
Alexa Fluor Plus 488	AF5254AFP488-100UG
Alexa Fluor Plus 555	AF5254AFP555-100UG
Alexa Fluor Plus 594	AF5254AFP594-100UG
Alexa Fluor Plus 647	AF5254AFP647-100UG
Alexa Fluor Plus 680	AF5254AFP680-100UG
Allophycocyanin	AF5254A
Biotin	AF5254B
CoraFluor 1	AF5254CL1
DyLight 350	AF5254D
DyLight 405	AF5254E
DyLight 488	AF5254K
DyLight 550	AF5254L
DyLight 594	AF5254M
DyLight 650	AF5254W
DyLight 680	AF5254Y
DyLight 755	AF5254Z
FITC	AF5254F
HRP	AF5254H
Janelia Fluor 525	AF5254JF525
Janelia Fluor 549	AF5254I
Janelia Fluor 646	AF5254J
Janelia Fluor 669	AF5254JF669
PE	AF5254P
PerCP	AF5254C
mFluor Violet 450 SE	AF5254MFV450
mFluor Violet 500 SE	AF5254MFV500
mFluor Violet 610 SE	AF5254MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5254-SP

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NBP2-37602

Cdk5 Antibody (4E4)

(2 Publications)

0.1 ml

NBP2-37602

Immunohistochemistry-Paraffin: Cdk5 Antibody (4E4) [NBP2-37602] - Analysis of cerebellum tissues (left) and brain tissues (right) using CDK5 mouse mAb with DAB staining.

Flow Cytometry: Cdk5 Antibody (4E4) [NBP2-37602] - Analysis of K562 cells using CDK5 mouse mAb (green) and negative control (purple).

ELISA: Cdk5 Antibody (4E4) [NBP2-37602] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu, Rt, Pm

Species Glossary

Reactivity: Hu, Rt, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Lysate Subcellular Fraction

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Survivin Antibody - BSA Free

CDK2 Antibody (OTI2D9)

Cdk6 Antibody - BSA Free

CDK4 Antibody - BSA Free

CDK2 Antibody [Unconjugated]

CDK4 Antibody (DCS-31)

CDK2 Antibody (1A6) - BSA Free

Cdk9 Antibody (1C4E3)

Cdk6 Antibody (8H4) - Azide and BSA Free

CDK4 Antibody (6N3M6)

CDK4 Antibody [Unconjugated]

Cdk5 Antibody (4E4)