NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="CD55/DAF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (left panel, positive staining) and MOLT-4 human acute lymphoblastic leukemia cell line (right panel, negative staining) using Goat Anti-Human CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2009) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF005). A specific band was detected for CD55/DAF at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for CD55/DAF at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse splenocytes. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse CD55/DAF Monoclonal Antibody (Catalog # MAB5376) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD55/DAF at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF005). A specific band was detected for CD55/DAF at approximately 75 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1. No bands were observed when using reducing conditions." class="big_lightbox"> HAF005). A specific band was detected for CD55/DAF at approximately 75 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1. No bands were observed when using reducing conditions." class="big_thumb" />

MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B)." class="big_lightbox"> MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B)." class="big_thumb" />

HAF016). A specific band was detected for CD55/DAF at approximately 75 kDa (as indicated). This experiment was conducted under reducing and non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for CD55/DAF at approximately 75 kDa (as indicated). This experiment was conducted under reducing and non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse spleen tissue and mouse splenocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5376) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD55/DAF at approximately 75 kDa (as indicated). This experiment was conducted under reducing and non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011)." class="big_lightbox"> 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011)." class="big_thumb" />

2529-CD), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human CD97 (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD55/DAF Monoclonal Antibody (Catalog # MAB2009). The ND₅₀ is typically 0.5-2 µg/mL." class="big_lightbox"> 2529-CD), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human CD97 (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD55/DAF Monoclonal Antibody (Catalog # MAB2009). The ND₅₀ is typically 0.5-2 µg/mL." alt="Recombinant Human CD97 (Catalog # 2529-CD), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human CD97 (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD55/DAF Monoclonal Antibody (Catalog # MAB2009). The ND₅₀ is typically 0.5-2 µg/mL." class="big_thumb" />