| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24709SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4324-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41308-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41307-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4324-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89044-25ul | |||
![Western Blot: ATG4B Antibody [NBP2-24709] - Analysis of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24709 at 2 ug/ml.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Western-Blot-NBP2-24709-img0001.jpg "Western Blot: ATG4B Antibody [NBP2-24709] - Analysis of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24709 at 2 ug/ml.")
![Immunohistochemistry-Paraffin: ATG4B Antibody [NBP2-24709] - Analysis of human heart using NBP2-24709 at 10 ug/ml.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Immunohistochemistry-Paraffin-NBP2-24709-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Goat Polyclonal ATG4D Antibody [NBP1-36939] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/antibody/nbp1-36939_goat-polyclonal-atg4d-antibody-immunocytochemistry-immunofluorescence-283202416418..jpg "Immunocytochemistry/Immunofluorescence: Goat Polyclonal ATG4D Antibody [NBP1-36939] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-36939] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunohistochemistry-Paraffin-NBP1-36939-img0001.jpg)
![Flow Cytometry: Goat Polyclonal ATG4D Antibody [NBP1-36939] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-36939_goat-polyclonal-atg4d-antibody-flow-cytometry-283202416538..jpg)
![Western Blot: ATG4C Antibody (JE59-99) [NBP3-32059] - Western blot analysis of ATG4C on different lysates with Rabbit anti-ATG4C antibody (NBP3-32059) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: Raji cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32059) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32059_rabbit-atg4c-mab-je59-99-278202416331241.jpg "Western Blot: ATG4C Antibody (JE59-99) [NBP3-32059] - Western blot analysis of ATG4C on different lysates with Rabbit anti-ATG4C antibody (NBP3-32059) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: Raji cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32059) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ATG4C Antibody (JE59-99) [NBP3-32059] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ATG4C antibody (NBP3-32059) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32059) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32059_rabbit-atg4c-mab-je59-99-298202414161840.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG4C Antibody (JE59-99) [NBP3-32059] - Immunocytochemistry analysis of Jurkat cells labeling ATG4C with Rabbit anti-ATG4C antibody (NBP3-32059) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATG4C antibody (NBP3-32059) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32059_rabbit-atg4c-mab-je59-99-29202410334039.jpg)
![Flow Cytometry: ATG4C Antibody (JE59-99) [NBP3-32059] - Flow cytometric analysis of Jurkat cells labeling ATG4C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32059, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32059_rabbit-atg4c-mab-je59-99-392024884633.jpg)
![Western Blot: ATG4D Antibody [NBP2-41308] - Analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Western-Blot-NBP2-41308-img0001.jpg "Western Blot: ATG4D Antibody [NBP2-41308] - Analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: ATG4D Antibody - BSA Free [NBP2-41308] - Immunofluorescence of ATG4D in human testis tissue with ATG4D antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp2-41308_rabbit-polyclonal-atg4d-antibody-2711202420115547.jpg)
![Immunohistochemistry: ATG4D Antibody - BSA Free [NBP2-41308] - Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41308_rabbit-polyclonal-atg4d-antibody-2711202419592031.jpg)
![Western Blot: ATG4B Antibody [NBP2-41307] - Analysis of ATG4B in (A) Ramos and (B) Jurkat cell lysate with ATG4B antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Western-Blot-NBP2-41307-img0003.jpg "Western Blot: ATG4B Antibody [NBP2-41307] - Analysis of ATG4B in (A) Ramos and (B) Jurkat cell lysate with ATG4B antibody at 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: ATG4B Antibody - BSA Free [NBP2-41307] - Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp2-41307_rabbit-polyclonal-atg4b-antibody-271120242016974.jpg)
![Immunohistochemistry: ATG4B Antibody - BSA Free [NBP2-41307] - Immunohistochemistry of ATG4B in human spleen tissue with ATG4B antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41307_rabbit-polyclonal-atg4b-antibody-2711202420272418.jpg)
![Western Blot: ATG4C Antibody [NBP1-89043] - Analysis in control (vector only transfected HEK293T lysate) and ATG4C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ATG4C-Antibody-Western-Blot-NBP1-89043-img0009.jpg "Western Blot: ATG4C Antibody [NBP1-89043] - Analysis in control (vector only transfected HEK293T lysate) and ATG4C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: ATG4C Antibody [NBP1-89043] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/ATG4C-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89043-img0008.jpg)
![Immunohistochemistry-Paraffin: ATG4C Antibody [NBP1-89043] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/ATG4C-Antibody-Immunohistochemistry-Paraffin-NBP1-89043-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ATG4D Antibody [NBP1-33488] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG4D protein stained by ATG4D antibody [N3C3] diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33488-img0006.jpg "Immunocytochemistry/Immunofluorescence: ATG4D Antibody [NBP1-33488] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG4D protein stained by ATG4D antibody [N3C3] diluted at 1:200.Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded rat muscle. ATG4D antibody [N3C3] diluted at 1:500.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunohistochemistry-Paraffin-NBP1-33488-img0005.jpg)
![Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded SAS xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunohistochemistry-Paraffin-NBP1-33488-img0003.jpg)
![Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded mouse ovary. ATG4D antibody [N3C3] diluted at 1:500.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunohistochemistry-Paraffin-NBP1-33488-img0004.jpg)
![Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538] - Western blot analysis of extracts of various cell lines, using ATG4B Rabbit mAb (NBP3-16538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ATG4B-Antibody-9H6M6-Western-Blot-NBP3-16538-img0002.jpg "Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538] - Western blot analysis of extracts of various cell lines, using ATG4B Rabbit mAb (NBP3-16538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] - Immunofluorescence analysis of NIH-3T3 cells using ATG4B Rabbit mAb (NBP3-16538) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATG4B-Antibody-9H6M6-Immunocytochemistry-Immunofluorescence-NBP3-16538-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] - Confocal imaging of NIH/3T3 cells using ATG4B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16538_rabbit-atg4b-mab-9h6m6-7220251245390.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] - Confocal imaging of paraffin-embedded Mouse brain using ATG4B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . Objective: 40x. DAPI was used for nuclear staining (Blue). Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16538_rabbit-atg4b-mab-9h6m6-7220251245398.jpg)
![Western Blot: ATG4C Antibody [NBP2-81954] - Analysis of ATG4C in A549 cell lysate with ATG4C antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/ATG4C-Antibody-Western-Blot-NBP2-81954-img0001.jpg "Western Blot: ATG4C Antibody [NBP2-81954] - Analysis of ATG4C in A549 cell lysate with ATG4C antibody at 1 ug/ml.")
![Immunohistochemistry: ATG4C Antibody [NBP2-81954] - Analysis of ATG4C in human lung tissue with ATG4C antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/ATG4C-Antibody-Immunohistochemistry-NBP2-81954-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG4C Antibody - BSA Free [NBP2-81954] - Immunofluorescence of ATG4C in human lung tissue with ATG4C antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81954_rabbit-polyclonal-atg4c-antibody-271120242041457.jpg)
![Western Blot: ATG4A Antibody [NBP1-89044] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-50](http://images.novusbio.com/fullsize/ATG4A-Antibody-Western-Blot-NBP1-89044-img0006.jpg "Western Blot: ATG4A Antibody [NBP1-89044] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-50")
![Immunohistochemistry-Paraffin: ATG4A Antibody [NBP1-89044] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/ATG4A-Antibody-Immunohistochemistry-Paraffin-NBP1-89044-img0002.jpg)
![Western Blot: ATG4A Antibody [NBP1-89044] -Lane 1: NIH-3T3 lysate (mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells.](http://images.novusbio.com/fullsize/antibody/nbp1-89044_rabbit-polyclonal-atg4a-antibody-western-blot-2792023133149..jpg)