VSIG1 Antibody (986709) [Unconjugated]

Western blot shows lysates of human stomach tissue and human testis tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VSIG1 Monoclonal Antibody (Catalog # MAB48182) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VSIG1 at approximately 50-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VSIG1 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human VSIG1 Monoclonal Antibody (Catalog # MAB48182) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of human stomach, loaded at 0.2 mg/mL. A specific band was detected for VSIG1 at approximately 56 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human VSIG1 Monoclonal Antibody (Catalog # MAB48182). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clone: 986709
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VSIG1 Antibody (986709) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human VSIG1
  Val22-Gly234
  Accession # Q86XK7
• Specificity: Detects human VSIG1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VSIG1 Antibody (986709) [Unconjugated]

• Cell surface A33 antigen
• dJ889N15.1
• Glycoprotein A34
• GPA34
• MGC44287,1700062D20Rik
• V-set and immunoglobulin domain containing 1
• V-set and immunoglobulin domain-containing protein 1
• VSIG1

Background

VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein A34) is a variably glycosylated 55‑70 kDa member of the JAM family of proteins. It has restricted expression, being limited to testicular germ cells plus pancreatic duct and gastric epithelium. VSIG1 is likely to serve as an adhesion molecule. Mature human VSIG1 is 366 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 211 aa extracellular domain (ECD). The ECD contains one V-type (aa 22‑132) and one C2-type Ig-like domain (aa 140‑227). Over aa 22‑234, human VSIG1 is 83% aa identical to both mouse and canine VSIG1. At least one potential splice variant exists in human. It shows an insertion of 36 aa after Ser72 and a deletion of aa 133‑387.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q86XK7()