Working with the V5 Tag Antibody

Fri, 06/10/2011 - 07:39

The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, with an extensive range of V5 tag antibody products on our antibody database.

An epitope tag is a short peptide sequence, which is bioengineered onto the N- or C- terminus of a protein so it can be recognized by the equivalent antibody, or antibody paratope (i.e. the area of the immunoglobulin receptive to that tag).

Western Blot: V5-TAG Antibody

Epitope tags are created using recombinant DNA technology, which allows the fusion of a short sequence of foreign peptides (such as from a virus) with the target gene’s DNA. When this protein is introduced into cells, the protein is expressed in its tagged form. The V5 tag antibody acts as a universal reagent for any recombinant protein carrying the V5 tag, thus avoiding the need to generate individual antibodies for specific proteins. V5 epitope tags are useful as their small size does not interfere with the biochemical properties of the proteins being studied.

The V5 epitope tag represents the amino acid residues 95-108, gly lys pro ile pro asn pro leu leu gly leu asp thr (GKPIPNPLLGLDST), of the RNA polymerase alpha subunit of the SV5 (simian virus 5) paramyxovirus. We have a number of conjugated and non-conjugated V5 tag antibody products on our antibody database. Our SV5-Pk V5 tag antibody products are specific to the small Pk epitope of the P and V proteins of the SV5 virus, and are used to identify membrane-bound and secreted proteins.

Novus Biologicals offers many V5 tag reagents for your research needs including:

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