Urotensin-II R/GPR14 Antibody (443707) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) mouse Urotensin-II R or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse Urotensin-II R Monoclonal Antibody (Catalog # MAB9245) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 443707
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Urotensin-II R/GPR14 Antibody (443707) [Unconjugated] Summary

• Immunogen: Mouse Urotensin-II R synthetic peptide
• Specificity: Detects mouse Urotensin-II R. Stains mouse Urotensin-II R transfectants but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Urotensin-II R/GPR14 Antibody (443707) [Unconjugated]

• G protein-coupled receptor 14
• GPR14
• G-protein coupled receptor 14
• UR-2-R
• UR-II-R
• urotensin 2 receptor
• urotensin II receptor
• urotensin-2 receptor
• Urotensin-II R
• UrotensinIIR
• Urotensin-IIR
• UTR
• UTR2
• UTS2R

Background

Urotensin-II R (UR2R) belongs to the G protein-coupled receptor 1 family. UR2R is a high affinity receptor for urotensin-2 and urotensin-2B. The activity of UR2R is mediated by a G protein that activates a phosphatidylinositol-calcium second messenger system. It is most abundantly expressed in the heart and pancreas. Human UR2R has been correlated with cortico-adrenal carcinoma proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.