TWF2 Overexpression Lysate

Images

 
Western Blot: TWF2 Overexpression Lysate (Adult Normal) [NBL1-17459] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TWF2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TWF2 Overexpression Lysate Summary

Description

TWF2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC201791

Accession#: NM_007284

Protein Tag: C-MYC/DDK
Gene
TWF2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
39.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TWF2 Overexpression Lysate

  • A6-related protein
  • A6RPFLJ56277
  • hA6RP
  • protein tyrosine kinase 9-like (A6-related protein)
  • Protein tyrosine kinase 9-like
  • PTK9L protein tyrosine kinase 9-like (A6-related protein)
  • PTK9LA6r
  • twinfilin, actin-binding protein, homolog 2 (Drosophila)
  • Twinfilin-1-like protein
  • twinfilin-2

Background

The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-90297
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
AF3966
Species: Hu
Applications: IHC, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP2-80461
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-49118
Species: Hu
Applications: IHC, IHC-P
NBP1-33177
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP2-82026
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-67895
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IP, WB
236-EG
Species: Hu
Applications: BA
AF2685
Species: Hu
Applications: IHC, Simple Western, WB
NB100-91266
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-00527
Species: Ca, Hu, Pm, Mu, Pm
Applications: Flow, ICC/IF, IHC, IHC-P, WB

Publications for TWF2 Lysate (NBL1-17459) (0)

There are no publications for TWF2 Lysate (NBL1-17459).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TWF2 Lysate (NBL1-17459) (0)

There are no reviews for TWF2 Lysate (NBL1-17459). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TWF2 Lysate (NBL1-17459) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TWF2 Products

Bioinformatics Tool for TWF2 Lysate (NBL1-17459)

Discover related pathways, diseases and genes to TWF2 Lysate (NBL1-17459). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Pathways for TWF2 Lysate (NBL1-17459)

View related products by pathway.

PTMs for TWF2 Lysate (NBL1-17459)

Learn more about PTMs related to TWF2 Lysate (NBL1-17459).
 

Research Areas for TWF2 Lysate (NBL1-17459)

Find related products by research area.

Blogs on TWF2

There are no specific blogs for TWF2, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TWF2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol TWF2