TUFM Antibody (900)

• Reactivity: Ec
• Applications: WB, ELISA, ICC/IF
• Clone: 900
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

TUFM Antibody (900) Summary

• Description: Novus Biologicals Mouse TUFM Antibody (900) (NBP3-11261) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: E. coli K-12 C600 Rif ( pKT146) bacteria
• Epitope: The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUFM
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for TUFM Antibody (900)

• EFTu
• EF-Tu
• EFTUCOXPD4
• EF-TuMT
• elongation factor Tu, mitochondrial
• P43
• Tu translation elongation factor, mitochondrial

Background

The monoclonal antibody 900 recognizes elongation factor Tu (EF-Tu), a 43 kDa multifunctional protein present in Escherichia coli. EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins. Monoclonal antibody 900 recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for diagnostic purposes. The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUFM