TSSK2 Antibody (1E12) - Azide and BSA Free

Western Blot: TSSK2 Antibody (1E12) [H00023617-M01] - TSSK2 monoclonal antibody (M01), clone 1E12 Analysis of TSSK2 expression in HL-60.

Western Blot: TSSK2 Antibody (1E12) [H00023617-M01] - Analysis of TSSK2 expression in transfected 293T cell line by TSSK2 monoclonal antibody (M01), clone 1E12.Lane 1: TSSK2 transfected lysate(41 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TSSK2 Antibody (1E12) - Azide and BSA Free Summary

• Immunogen: TSSK2 (AAH37781, 1 a.a. ~ 358 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDDATVLRKKGYIVGINLGKGSYAKVKSAYSERLKFNVAVKIIDRKKTPTDFVERFLPREMDILATVNHGSIIKTYEIFETSDGRIYIIMELGVQGDLLEFIKCQGALHEDVARKMFRQLSSAVKYCHDLDIVHRDLKCENLLLDKDFNIKLSDFGFSKRCLRDSNGRIILSKTFCGSAAYAAPEVLQSIPYQPKVYDIWSLGVILYIMVCGSMPYDDSDIRKMLRIQKEHRVDFPRSKNLTCECKDLIYRMLQPDVSQRLHIDEILSHSWLQPPKPKATSSASFKREGEGKYRAECKLDTKTDLRPDHRPDHKLGAKTQHRLLVVPENENRMEDRLAETSRAKDHHISGAEVGKAST
• Specificity: TSSK2 - testis-specific serine kinase 2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSSK2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA. Per customer report, additional use in immunocytochemistry/immunofluorescence and Immunohistochemistry.

Reactivity Notes

Cross reacts with Mouse sperm (see protduct review). Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TSSK2 Antibody (1E12) - Azide and BSA Free

• DGSG
• DiGeorge syndrome protein G
• EC 2.7.11
• FLJ38613
• serine/threonine kinase 22B (spermiogenesis associated)
• Serine/threonine-protein kinase 22B
• spermiogenesis associated 2
• SPOGA2EC 2.7.11.1
• STK22BDGS-G
• testis specific serine threonine kinase 2
• Testis-specific kinase 2
• testis-specific serine kinase 2
• testis-specific serine/threonine-protein kinase 2
• TSK-2
• TSSK-2

Background

TSSK2 belongs to a family of serine/threonine kinases highly expressed in testis (Hao et al., 2004 [PubMed 15044604]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSSK2 Antibody (H00023617-M01)(2)

Publications using H00023617-M01

• Jha KN, Coleman AR, Wong L et al. HSP90 functions to stabilize and activate the testis-specific serine/threonine kinases, a family of kinases essential for male fertility. J Biol Chem 2013-04-18 [PMID: 23599433] (WB, Human, Mouse)
• Li Y, Sosnik J, Brassard L et al. Expression and Localization of Five Members of the Testis-Specific Serine Kinase (Tssk) Family in Mouse and Human Sperm and Testis. Mol Hum Reprod 2010-08-20 [PMID: 20729278] (IF/IHC, ICC/IF, WB, Mouse, Human)

Review for TSSK2 Antibody (H00023617-M01) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Ana Salicioni WB Mouse 10/19/2010

Summary

• Application: Western Blot
• Sample Tested: mouse and human sperm, Sample Amount: 50 ug
• Species: Mouse

Bioinformatics

• Gene Symbol: TSSK2
• Entrez:
  • 23617(Human)
• Uniprot:
  • AAH37781(Human)