TREML4/TLT-4 Antibody (817914) [Unconjugated]

CD8+ CD11c+ mouse splenocytes were stained with Rat Anti-Mouse TREML4/TLT‑4 Monoclonal Antibody (Catalog # MAB5500, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 817914
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TREML4/TLT-4 Antibody (817914) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TREML4/TLT-4
  Gly25-Pro195
  Accession # Q3LRV9
• Specificity: Detects mouse TREML4/TLT-4 in ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Treml4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREML4/TLT-4 Antibody (817914) [Unconjugated]

• TLT4
• TREML4

Background

TREML4 (Triggering Receptor Expressed on Myeloid cells-Like 4), also known as TLT4, is a 53-55 kDa member of the Ig superfamily.  It is likely expressed by leukocytes, and based on its structure, may act as an activating receptor.  Mature mouse TREML4 is a type I transmembrane (TM) glycoprotein that is 235 amino acids (aa) in length.  It contains a 171 aa extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the TM segment contains one Lys residue.  There is one splice variant that shows a deletion of aa 21-24, a segment that lies in the putative signal sequence.  Rodent and human TREML4 are highly divergent, and thus over aa 25-195, mouse TREML4 shares 78% aa sequence identity with rat TREML4, but only 52% aa sequence identity with human TREML4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Treml4
• Uniprot:
  • Q3LRV9()