TLR Downstream Antibody Pack

Images

 
Western Blot: TLR Downstream Antibody Pack [NBP2-25084] - Analysis of MD-2 in A) human, B) mouse, and C) rat spleen lysate using NBP2-25084 at 2 ug/ml.
Immunohistochemistry-Paraffin: TLR Downstream Antibody Pack [NBP2-25084] - Analysis of MD-2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and NBP2-25084 (bottom left, right) ...read more
Western Blot: TLR Downstream Antibody Pack [NBP2-25084] - Analysis of IRAK-2 in A) human K562 and B) mouse Rawcell lysate using NBP2-25084 at 3 ug/ml.

Product Details

Summary
Applications WB, IHC-P

Order Details

TLR Downstream Antibody Pack Summary

Immunogen
MyD88: 25 ug: WB: H, M, R: Purified
NF-kB (p65): 25 ug: WB, Flow, IHC: H, M, R: Purified
MD-1: 25 ug: WB, IP: H, M: Purified
MD-2: 25 ug: WB, IHC: H, M, R: Purified
IRAK-1: 25 ug: WB, IP: H, M: Purified
IRAK-2: 25 ug: WB: H, M: Purified
IRAK-4: 25 ug: WB, IP: H, M: Purified
WB: Western Blot, IHC: Immunohistochemistry, IP: Immunoprecipitation, H: Human M: Mouse, R: Rat
Purity
Protein G purified

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Purity
Protein G purified

Kit Components

Alternate Names for TLR Downstream Antibody Pack

  • Toll like receptors
  • Toll-like receptors

Background

Detection of PAMPs by TLRs represents the immune recognition step that accounts for self-nonself discrimination, triggers the innate immune response, and act as efficient molecules for priming and optimizing antigen specific adaptive host response. The TLR gene family and their pathways have been evolutionarily well conserved in both invertebrates and vertebrates. Among the various molecules involved in the successful coordination of the TLR pathway the most important is the adapter protein MyD88 that acts via IRAK1, IRAK2 and TRAF6. Stimulation of MyD88 leads to association with the IL-1R associated kinases, IRAK1, IRAK2 and IRAK4, where IRAK4 is regarded as the central mediator of TLR signaling which acts by activating IRAK1. Activation by these molecules leads to phosphorylation and degradation of IkB leading to the consequent release of NF-kB (p65) and NF-kB (p50). Thus stimulation of TLR by its cognate ligands leads to activation of the NF-kappa-B pathway, cytokine secretion and inflammatory response. TLR4 signaling may require additional molecules like MD-1, important for efficient CD180 cell surface expression and MD-2 to respond to LPS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
Supplier Logo

Publications for TLR Downstream Antibody Pack (NBP2-25084) (0)

There are no publications for TLR Downstream Antibody Pack (NBP2-25084).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TLR Downstream Antibody Pack (NBP2-25084) (0)

There are no reviews for TLR Downstream Antibody Pack (NBP2-25084). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TLR Downstream Antibody Pack (NBP2-25084) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TLR Downstream Products

TLR Downstream NBP2-25084

Blogs on TLR Downstream

There are no specific blogs for TLR Downstream, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our TLR Downstream Antibody Pack and receive a gift card or discount.