Recombinant Human THG1L His Protein

SDS-Page: THG1L Protein [NBP2-22890]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human THG1L His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 30-298 of Human THG1L
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAKSKFE YVRDFEADDT CLAHCWVVVR LDGRNFHRFA EKHNFAKPND SRALQLMTKC AQTVMEELED IVIAYGQSDE YSFVFKRKTN WFKRRASKFM THVASQFASS YVFYWRDYFE DQPLLYPPGF DGRVVVYPSN QTLKDYLSWR QADCHINNLY NTVFWALIQQ SGLTPVQAQG RLQGTLAADK NEILFSEFNI NYNNELPMYR KGTVLIWQKV DEVMTKEIKL PTEMEGKKMA VTRTRTKPVP LHCDIIGDAF WKEHPEILDE DS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: THG1L
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human THG1L His Protein

• EC 2.7.7
• EC 2.7.7.6
• FLJ11601
• FLJ20546
• ICF45EC 2.7.7.-
• Interphase cytoplasmic foci protein 45
• probable tRNA(His) guanylyltransferase
• tRNA-histidine guanylyltransferase 1-like (S. cerevisiae)
• tRNA-histidine guanylyltransferase

Background

Probable tRNA (His) guanylyltransferase, also known as THG1L, belongs to the tRNA (His) gyanylytransferase family. Localized to the cytoplasm, THG1L is also found near the nuclear membrane and is expressed in many tissues, including liver and lung. Upon DNA damage, THG1L is phosphorylated, most likely by ATM or ATR. It is expressed in a cell cycle-dependent manner and may be involved in cell cycle progression and cell proliferation. Recombinant human THG1L protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: THG1L