TDRD1 Antibody

Western blot shows lysates of mouse testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse TDRD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7320) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TDRD1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

TDRD1 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse TDRD1
  Asn831-Lys960
  Accession # Q99MV1
• Specificity: Detects mouse TDRD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant human TDRD1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TDRD1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TDRD1 Antibody

• CT41.1
• CT41.1Cancer/testis antigen 41.1
• FLJ21082
• TDRD1
• tudor domain containing 1
• tudor domain containing protein 1
• tudor domain-containing protein 1

Background

TDRD1 (Tudor domain containing protein 1; also MTR-1) is a 130-145 kDa member of the Tudor family of proteins. It is expressed in spermatocytes and spermatids, and forms part of an RNA-silencing complex that regulates gene expression. TDRD1, through its Tudor and MYND domains, binds methylated PIWI (P-element-induced wimpy testis) protein and PIWI-interacting RNA loading factor, respectively. This serves to promote the recruitment of germline-specific small RNAs into a PIWI ribonucleoprotein complex. Mouse TDRD1 is 1172 aa in length. It contains one MYND-type Zn finger region (aa 163-199), and four methyl-binding Tudor domains (aa 307-1032). Over aa 831-960, mouse TDRD1 shares 96% and 66% aa identity with rat and human TDRD1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TDRD1
• Uniprot:
  • Q99MV1(Mouse)