STK33 Antibody (6G1) - Azide and BSA Free

Western Blot: STK33 Antibody (6G1) [H00065975-M02] - STK33 monoclonal antibody (M02), clone 6G1 Analysis of STK33 expression in HeLa.

Immunocytochemistry/Immunofluorescence: STK33 Antibody (6G1) [H00065975-M02] - Analysis of monoclonal antibody to STK33 on HeLa cell. Antibody concentration 25 ug/ml.

Western Blot: STK33 Antibody (6G1) [H00065975-M02] - Analysis of STK33 over-expressed 293 cell line, cotransfected with STK33 Validated Chimera RNAi ( Cat # H00065975-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STK33 monoclonal antibody (M02) clone 6G1 (Cat # H00065975-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: STK33 Antibody (6G1) [H00065975-M02] - Analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody (M02), clone 6G1.Lane 1: STK33 transfected lysate(57.9 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: STK33 Antibody (6G1) [H00065975-M02] - Analysis of STK33 transfected lysate using anti-STK33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK33 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: STK33 Antibody (6G1) [H00065975-M02] - Detection limit for recombinant GST tagged STK33 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP, Mycoplasma
• Clone: 6G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

STK33 Antibody (6G1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse STK33 Antibody (6G1) - Azide and BSA Free (H00065975-M02) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-STK33 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: STK33 (AAH31231, 1 a.a. ~ 514 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADSGLDKKSTKCPDCSSASQKDVLCVCSSKTRVPPVLVVEMSQTSSIGSAESLISLERKKEKNINRDITSRKDLPSRTSNVERKASQQQWGRGNFTEGKVPHIRIENGAAIEEIYTFGRILGKGSFGIVIEATDKETETKWAIKKVNKEKAGSSAVKLLEREVNILKSVKHEHIIHLEQVFETPKKMYLVMELCEDGELKEILDRKGHFSENETRWIIQSLASAIAYLHNNDIVHRDLKLENIMVKSSLIDDNNEINLNIKVTDFGLAVKKQSRSEAMLQATCGTPIYMAPEVISAHDYSQQCDIWSIGVVMYMLLRGEPPFLASSEGKLFELIRKGELHFENAVWNSISDCAKSVLKQLMKVDPAHRITAKELLDNQWLTGNKLSSVRPTNVLEMMKEWKNNPESVEENTTEEKNKPSTEEKLKSYQPWGNVPETNYTSDEEEEKQSTTYEKQFPATSKDNFDMCSSSFTSSKLLPAEIKGEMEKTPVTPSQGTATKYPAKSGALSRTKKKL
• Specificity: STK33 - serine/threonine kinase 33
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STK33
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, RNAi validation, and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STK33 Antibody (6G1) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.1
• serine/threonine kinase 33
• serine/threonine-protein kinase 33
• STK33

Background

Serine/threonine Kinase 33 (STK33) is expressed at high levels in testis and the fetal lung and heart, and at lower levels in a number of other tissues. However, STK33 is different form the other serine/threonine kinases in that it is not expressed in neural tissues. STK33 is thought to be involved in the phosphorylation of vimentin. Additionally, STK33 has been identified as a synthetic lethal in KRAS mutant lung cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STK33 Antibody (H00065975-M02)(1)

Publication using H00065975-M02

• Scholl C, Frohling S, Dunn IF et al. Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells. Cell. 2009-05-29 [PMID: 19490892]

Bioinformatics

• Gene Symbol: STK33
• Entrez:
  • 65975(Human)
• OMIM:
  • 607670(Human)
• Uniprot:
  • AAH31231(Human)