SRCRB4D Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SRCRB4D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5654) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SRCRB4D at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SRCRB4D Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human SRCRB4D isoform 1
  Ala45-Ser575
  Accession # Q8WTU2
• Specificity: Detects human SRCRB4D in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human CD163 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SRCRB4D
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SRCRB4D Antibody [Unconjugated]

• S4D-SRCRB
• S4-SRCRB
• scavenger receptor cysteine rich domain containing, group B (4 domains)
• scavenger receptor cysteine-rich domain-containing group B protein
• scavenger receptor cysteine-rich protein SRCRB-S4D
• SRCRB4D
• SRCRB-S4D
• SSC4D

Background

AMSH (Associated molecule with SH3 domain of STAM) is a 50 kDa member of the peptidase M67C class of enzymes. It is widely expressed and serves two functions. First, it promotes receptor recycling by counteracting the effects of ubiquitinating enzymes, and second, it participates in cytokine signal transduction by forming a complex with Jak2/3, STAM and the common beta ‑ and gamma ‑chains. Human AMSH is 424 amino acids (aa) in length. It contains an NLS (aa 113‑127), two SH3‑binding motifs (aa 195‑198 and 227‑231), and one MPN domain (aa 252‑361). There are five phosphorylation sites at Ser2, 48, 243, 245 and 247. AMSH has one potential splice variant that shows a 17 aa substitution for aa 1‑68. Full‑length human AMSH is 83% aa identical to mouse AMSH.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRCRB4D
• Uniprot:
  • Q8WTU2()