Recombinant Human Snapin His Protein

SDS-Page: SNAPIN Protein [NBC1-22575] - SNAPAP, 17 kDa (156aa), confirmed by MALDI-TOF with a purity of 90% by SDS-PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human Snapin His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-136 of Human Snapin
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAGAGSAAVS GAGTPVAGPT GRDLFAEGLL EFLRPAVQQL DSHVHAVRES QVELREQIDN LATELCRINE DQKVALDLDP YVKKLLNARR RVVLVNNILQ NAQERLRRLN HSVAKETARR RAMLDSGIYP PGSPGK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SNAPIN
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 5 mM DTT, 2 mM EDTA, 0.2 M NaCl2, 40% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Snapin His Protein

• SNAP-25-binding protein
• SNAP25BP
• SNAPAP
• SNAPAPSNARE-associated protein Snapin
• SNAP-associated proteinSNARE associated protein snapin
• Snapin
• Synaptosomal-associated protein 25-binding protein

Background

SNAPAP (SNAP associated protein) was enriched in neurons and exclusively located on synaptic vesicle membrane protein. SNAPAP is an important component of the neurotransmitter release process through its modulation of the sequential interactions between the SNAREs and synaptotagmin, which is a component of the SNARE complex that is required for synaptic vesicle docking and fusion. Recombinant human SNAPAP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SNAPIN