Semaphorin 4F Antibody (780225) [Allophycocyanin]

Mouse cortical stem cells (A) and bEnd.3 mouse endothelioma cell line (B) were stained with Rat Anti-Mouse Semaphorin 4F APC-conjugated Monoclonal Antibody (Catalog # FAB7200A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

Semaphorin 4F Antibody (780225) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4F
  Arg41-Gly667
  Accession # Q9Z123
• Specificity: Detects mouse Semaphorin 4 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SEMA4F
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8degrees C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4F Antibody (780225) [Allophycocyanin]

• M-SEMA
• m-Sema-M
• PRO2353
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4F
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4F
• Sema M
• Sema W
• SEMA4F
• SEMAM
• Semaphorin 4F
• semaphorin-4F
• semaphorin-M
• semaphorin-W
• SEMAW
• SEMAWsemaphorin M

Background

Semaphorin 4F (Sema4F), also known as Sema W, is a 90-100 kDa class IV member of the semaphorin family of proteins. It is expressed by select cell types, including Schwann cells and neurons, and likely acts as an inhibitor of cell proliferation by Schwann cells, and a regulator of postsynaptic glutamatergic synapses on neuron dendrites. Mature mouse Sema4F is a type I transmembrane protein that is 737 amino acids (aa) in length. It contains a 627 aa extracellular region (aa 41-667) that is characterized by the presence of one Sema domain (aa 71-493), a PSI region (aa 518-569), and a C2-type Ig-like domain (aa 586-641). Over aa 41-667, mouse Sema4F shares 92% and 98% aa sequence identity with human and rat Sema4F, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA4F
• Uniprot:
  • Q9Z123(Mouse)