SARS Nucleocapsid Protein Antibody

Images

 
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): ...read more
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. ...read more
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Characteristics of 2B4 cells clonally derived from human bronchial epithelial Calu-3 cells. Finally, 2B4 cells (passage 6) were infected with ...read more
Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody.

Product Details

Summary
Reactivity V, VSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SARS Nucleocapsid Protein Antibody Summary

Immunogen
The antibody was developed by immunizing Rabbit with a synthetic peptide corresponding to amino acids 399-411 (DLDDFSKQLQQSM-C) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)
Specificity
Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Nucleocapsid protein (NBP2-90975) and low/no reactivity towards H1N1 (NBP1-99041). No cross-reactivity observed with influenza A(H1N1) virus, influenza B virus, respiratory syncytial virus, parainfluenza virus type 3, human coronavirus (HCoV) 229E, or MERS-CoV in PCR-confirmed tissue samples [PMID:32437316].
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
N
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Immunocytochemistry/Immunofluorescence: see Mossel et al (2008) for details. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24725942). Use in Immunohistochemistry reported in multiple pieces of scientific literature including Sars-CoV-2 in bioRxiv March 21, 2020 doi: https://doi.org/10.1101/2020.03.21.001628 and (PMID: 32396922)
Control
Recombinant SARS-CoV-2 Nucleocapsid Protein
Publications
Read Publications using
NB100-56576 in the following applications:

Reactivity Notes

Expected species cross reactivity based on immunogen sequence homology: COVID-19 (77%). SARS-CoV-2 data from bioRxiv March 21, 2020 //doi.org/10.1101/2020.03.21.001628.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for SARS Nucleocapsid Protein Antibody

  • 2019-nCoV Nucleocapsid Protein
  • COVID-19 N protein
  • N protein
  • N structural protein
  • N
  • NC
  • Nucleocapsid protein
  • Nucleoprotein
  • Protein N
  • SARS coronavirus N protein
  • SARS coronavirus nucleocapsid protein
  • SARS CoV N protein
  • SARS CoV nucleocapsid protein
  • SARS CoV
  • SARS N protein
  • SARS Nucleoprotein
  • SARSCoV N protein
  • SARSCoV nucleocapsid protein
  • SARSCoV
  • SARS-CoV-2 N protein
  • Severe acute respiratory syndrome

Background

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for SARS Nucleocapsid Protein Antibody (NB100-56576)(19)

We have publications tested in 7 confirmed species: Human, Mouse, Primate, SARS-CoV, SARS-CoV-2, Viral, Virus.

We have publications tested in 5 applications: Dual ISH-IHC, ICC/IF, IHC, IHC-P, WB.


Filter By Application
Dual ISH-IHC
(1)
ICC/IF
(1)
IHC
(5)
IHC-P
(6)
WB
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Primate
(1)
SARS-CoV
(1)
SARS-CoV-2
(3)
Viral
(1)
Virus
(3)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB100-56576 Applications Species
Jureka AS, Silvas JA, Basler CF Propagation, inactivation, and safety testing of SARS-CoV-2 bioRxiv Jan 1 2020 [PMID: 32517266] (WB, ICC/IF, Primate) WB, ICC/IF Primate
Martines R, Ritter J, Matkovic E et al. Pathology and Pathogenesis of SARS-CoV-2 Associated With Fatal Coronavirus Disease, United States Emerg Infect Dis. 020 May 21 [PMID: 32437316] (IHC, SARS-CoV, SARS-CoV-2) IHC SARS-CoV, SARS-CoV-2
van Doremalen N, Schafer A, Menachery VD et al. SARS-Like Coronavirus WIV1-CoV Does Not Replicate in Egyptian Fruit Bats (Rousettus aegyptiacus) Viruses Dec 19 2018 [PMID: 30572566] (IHC-P, Viral) IHC-P Viral
Fehr AR, Channappanavar R, Jankevicius G et al. The Conserved Coronavirus Macrodomain Promotes Virulence and Suppresses the Innate Immune Response during Severe Acute Respiratory Syndrome Coronavirus Infection. MBio. Dec 13 2016 [PMID: 27965448] (WB, Human) WB Human
Yasui F, Kohara M, Kitabatake M et al. Phagocytic cells contribute to the antibody-mediated elimination of pulmonary-infected SARS coronavirus. Virology 2014 Apr 01 [PMID: 24725942] (IHC-P, Mouse)

Details:
Lung tissue from SARs-CoV-infected mice,Fig 1C.
IHC-P Mouse
Munster V, Feldmann F, Williamson B et al. Respiratory disease and virus shedding in rhesus macaques inoculated with SARS-CoV-2 bioRxiv 2020 Mar 21 (IHC-P, Virus)

Details:
To evaluate the use of rhesus macaques as a model for SARS-CoV-2, eight adult rhesus macaques (4 males, 4 females) were inoculated with the virus
IHC-P Virus
Chandrashekar A, Liu J, Martinot A et al. SARS-CoV-2 Infection Protects Against Rechallenge in Rhesus Macaques Science. 2020 May 20 [PMID: 32434946] (IHC-P, IHC, Dual ISH-IHC, SARS-CoV-2)

Details:
SARS-CoV2 anti-sense specific probe v-nCoV2019-S (ACD Cat. No. 848561) targeting the positive-sense viral RNA, SARS-CoV2 sense specific probe vnCoV2019-orf1ab-sense (ACD Cat. No. 859151) targeting the negative-sense genomic viral RNA
IHC-P, IHC, Dual ISH-IHC SARS-CoV-2
Munster V, Feldmann F, Williamson B et al. Respiratory Disease in Rhesus Macaques Inoculated With SARS-CoV-2 Nature. 2020 May 12 [PMID: 32396922] (IHC-P, SARS-CoV-2)

Details:
Validation of cross-reactivity of SARS-CoV to SARS-CoV-2 in IHC was done in-house by embedding SARS-CoV-2 infected Vero cells in histogel and producing and staining histology slides.
IHC-P SARS-CoV-2
Channappanavar R, Fett C, Mack M et al. Sex-Based Differences in Susceptibility to Severe Acute Respiratory Syndrome Coronavirus Infection. J. Immunol. Apr 3 2017 [PMID: 28373583] (IHC, Virus) IHC Virus
Channappanavar R, Fehr AR, Vijay R et al. Dysregulated Type I Interferon and Inflammatory Monocyte-Macrophage Responses Cause Lethal Pneumonia in SARS-CoV-Infected Mice Cell Host Microbe Feb 10 2016 [PMID: 26867177] (IHC-P, Virus) IHC-P Virus
Show All 19 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SARS Nucleocapsid Protein Antibody (NB100-56576). (Showing 1 - 1 of 1 FAQs).

  1. does your antibody NB100-56576 recognizes SARS-CoV-2 or only SARS-Cov? (Image 1 indicates Cov2 in the legend, but the data sheet only states SARS coronavirus
    • This antibody was originally developed against SARS, but due to identity to SARS-CoV-2 was predicted to cross-react. the image in question (#1) is from a recent publication that used NB100-56576 to detect SARS-CoV-2 nucleocapsid. Here is the link to the publication if you would like to take a look https://www.biorxiv.org/content/10.1101/2020.03.21.001628v1

Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol N