SARS Nucleocapsid Protein Antibody - BSA Free

Images

 
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): ...read more
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. ...read more
Orthogonal Strategies: Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56576] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for ...read more
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Characteristics of 2B4 cells clonally derived from human bronchial epithelial Calu-3 cells. Finally, 2B4 cells (passage 6) were infected with ...read more
Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - Western blot shows recombinant SARS-CoV-2 Nucleocapsid protein. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-SARS-CoV-2 Nucleocapsid Polyclonal ...read more
Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody.

Product Details

Summary
Reactivity V, V, V-ViSpecies Glossary
Applications WB, ICC/IF, IHC, PAGE, Dual ISH-IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
       

Orthogonal Strategies

 

Order Details

SARS Nucleocapsid Protein Antibody - BSA Free Summary

Immunogen
The antibody was developed by immunizing Rabbit with a synthetic peptide corresponding to amino acids 399-411 (DLDDFSKQLQQSM-C) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)
Specificity
Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Nucleocapsid protein (NBP2-90975) and low/no reactivity towards H1N1 (NBP1-99041). No cross-reactivity observed with influenza A(H1N1) virus, influenza B virus, respiratory syncytial virus, parainfluenza virus type 3, human coronavirus (HCoV) 229E, or MERS-CoV in PCR-confirmed tissue samples [PMID:32437316].
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
N
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500. Use reported in scientific literature (Mossel et al (2008))
  • Immunohistochemistry reported in scientific literature (PMID 32396922)
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 24725942)
  • SDS-Page reported in scientific literature (PMID 34880383)
  • Western Blot 1:100-1:2000
Control
Recombinant SARS-CoV-2 Nucleocapsid His (C-Term) Protein
Publications
Read Publications using
NB100-56576 in the following applications:

Reactivity Notes

Use in SARS-CoV-2 reported in scientific literature (PMID:33807059).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SARS Nucleocapsid Protein Antibody - BSA Free

  • 2019-nCoV Nucleocapsid Protein
  • COVID-19 N protein
  • N protein
  • N structural protein
  • N
  • NC
  • Nucleocapsid protein
  • Nucleoprotein
  • Protein N
  • SARS coronavirus N protein
  • SARS coronavirus nucleocapsid protein
  • SARS CoV N protein
  • SARS CoV nucleocapsid protein
  • SARS CoV
  • SARS N protein
  • SARS Nucleoprotein
  • SARSCoV N protein
  • SARSCoV nucleocapsid protein
  • SARSCoV
  • SARS-CoV-2 N protein
  • Severe acute respiratory syndrome

Background

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for SARS Nucleocapsid Protein Antibody (NB100-56576)(148)

We have publications tested in 6 confirmed species: Human, Hamster, SARS-CoV, SARS-CoV-2, Viral, Virus.

We have publications tested in 16 applications: Dual ISH-IHC, ELISA, ELISPOT, EM, ICC/IF, IF/ICC, IF/IHC, IHC, IHC-Fr, IHC-P, ISH, In Vivo, MS, RNAScope, SDS-Page, WB.


Filter By Application
Dual ISH-IHC
(1)
ELISA
(2)
ELISPOT
(1)
EM
(1)
ICC/IF
(9)
IF/ICC
(1)
IF/IHC
(30)
IHC
(15)
IHC-Fr
(3)
IHC-P
(45)
ISH
(1)
In Vivo
(1)
MS
(1)
RNAScope
(1)
SDS-Page
(1)
WB
(16)
All Applications
Filter By Species
Human
(1)
Hamster
(1)
SARS-CoV
(1)
SARS-CoV-2
(21)
Viral
(1)
Virus
(7)
All Species
Showing Publications 1 - 10 of 148. Show All 148 Publications.
Publications using NB100-56576 Applications Species
Schiffler F, Moreira S, Pereira A et al. Lessons from a multilaboratorial task force for diagnosis of a fatal toxoplasmosis outbreak in captive primates in Brazil bioRxiv 2023-09-28 [PMID: 38138032] (IHC) IHC
Montagutelli X, Prot M, Levillayer L et al. Variants with the N501Y mutation extend SARS-CoV-2 host range to mice, with contact transmission bioRxiv 2021-03-19
Heise M, Dillard J, Taft-Benz S et al. Adjuvant-dependent effects on the safety and efficacy of inactivated SARS-CoV-2 vaccines during heterologous infection by a SARS-related coronavirus Research Square 2023-10-27 [PMID: 37961507] (IHC) IHC
Pepe-Mooney BJ, Smith CJ, Sherman MS et al. SARS-CoV-2 viral liver aggregates and scarce parenchymal infection implicate systemic disease as a driver of abnormal liver function Hepatology communications 2023-11-01 [PMID: 37889528] (IF/ICC) IF/ICC
Sauve F, Nampoothiri S, Clarke SA et al. Long-COVID cognitive impairments and reproductive hormone deficits in men may stem from GnRH neuronal death EBioMedicine 2023-09-13 [PMID: 37713808]
Delval L, Hantute-Ghesquier A, Sencio V et al. Removal of senescent cells reduces the viral load and attenuates pulmonary and systemic inflammation in SARS-CoV-2-infected, aged hamsters Nature Aging 2023-07-06 [PMID: 37414987]
Prakash S, Dhanushkodi N, Zayou L et al. Cross-Protection Induced by Highly Conserved Human B, CD4+,and CD8+T Cell Epitopes-Based Coronavirus Vaccine Against Severe Infection, Disease, and Death Caused by Multiple SARS-CoV-2 Variants of Concern bioRxiv 2023-05-25 [PMID: 37292861]
Miyamura S, Oe R, Nakahara T, Okada S Rapid detection of SARS-CoV-2 nucleocapsid protein using dual-comb biosensing arXiv
de Melo GD, Perraud V, Alvarez F et al. Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variants Nature communications 2023-07-26 [PMID: 37495586] (IHC-P) IHC-P
Goh D, Lim J, Fernandez S et al. Persistence of residual SARS-CoV-2 viral antigen and RNA in tissues of patients with long COVID-19 Research Square 4459-01-01 (IF/IHC, SARS-CoV-2) IF/IHC SARS-CoV-2
Show All 148 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SARS Nucleocapsid Protein Antibody (NB100-56576). (Showing 1 - 1 of 1 FAQs).

  1. does your antibody NB100-56576 recognizes SARS-CoV-2 or only SARS-Cov? (Image 1 indicates Cov2 in the legend, but the data sheet only states SARS coronavirus
    • This antibody was originally developed against SARS, but due to identity to SARS-CoV-2 was predicted to cross-react. the image in question (#1) is from a recent publication that used NB100-56576 to detect SARS-CoV-2 nucleocapsid. Here is the link to the publication if you would like to take a look https://www.biorxiv.org/content/10.1101/2020.03.21.001628v1
mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol N