Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): ...read more
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. ...read more
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Characteristics of 2B4 cells clonally derived from human bronchial epithelial Calu-3 cells. Finally, 2B4 cells (passage 6) were infected with ...read more
Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody.
The antibody was developed by immunizing Rabbit with a synthetic peptide corresponding to amino acids 399-411 (DLDDFSKQLQQSM-C) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)
Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Nucleocapsid protein (NBP2-90975) and low/no reactivity towards H1N1 (NBP1-99041). No cross-reactivity observed with influenza A(H1N1) virus, influenza B virus, respiratory syncytial virus, parainfluenza virus type 3, human coronavirus (HCoV) 229E, or MERS-CoV in PCR-confirmed tissue samples [PMID:32437316].
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunocytochemistry/Immunofluorescence: see Mossel et al (2008) for details. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24725942). Use in Immunohistochemistry reported in multiple pieces of scientific literature including Sars-CoV-2 in bioRxiv March 21, 2020 doi: https://doi.org/10.1101/2020.03.21.001628 and (PMID: 32396922)
Expected species cross reactivity based on immunogen sequence homology: COVID-19 (77%). SARS-CoV-2 data from bioRxiv March 21, 2020 //doi.org/10.1101/2020.03.21.001628.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for SARS Nucleocapsid Protein Antibody
2019-nCoV Nucleocapsid Protein
COVID-19 N protein
N structural protein
SARS coronavirus N protein
SARS coronavirus nucleocapsid protein
SARS CoV N protein
SARS CoV nucleocapsid protein
SARS N protein
SARSCoV N protein
SARSCoV nucleocapsid protein
SARS-CoV-2 N protein
Severe acute respiratory syndrome
It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for SARS Nucleocapsid Protein Antibody (NB100-56576)(19)
We have publications tested in 7 confirmed species: Human, Mouse, Primate, SARS-CoV, SARS-CoV-2, Viral, Virus.
We have publications tested in 5 applications: Dual ISH-IHC, ICC/IF, IHC, IHC-P, WB.
FAQs for SARS Nucleocapsid Protein Antibody (NB100-56576). (Showing 1 - 1 of 1 FAQs).
does your antibody NB100-56576 recognizes SARS-CoV-2 or only SARS-Cov? (Image 1 indicates Cov2 in the legend, but the data sheet only states SARS coronavirus
This antibody was originally developed against SARS, but due to identity to SARS-CoV-2 was predicted to cross-react. the image in question (#1) is from a recent publication that used NB100-56576 to detect SARS-CoV-2 nucleocapsid. Here is the link to the publication if you would like to take a look https://www.biorxiv.org/content/10.1101/2020.03.21.001628v1
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.