SARS Nucleocapsid Protein Antibody

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Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody.

Product Details

Summary
Reactivity Hu, VSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SARS Nucleocapsid Protein Antibody Summary

Immunogen
The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 399-411 of putative SARS nucleocapsid (Genbank accession no. NP_828858).
Specificity
SARS-Nucleocapsid protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
N
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Immunocytochemistry/Immunofluorescence: see Mossel et al (2008) for details Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24725942). Use in Immunohistochemistry reported in multiple pieces of scientific literature
Publications
Read Publications using
NB100-56576 in the following applications:

  • IHC
    3 publications
  • 2 publications
  • WB
    3 publications

Reactivity Notes

Cross reacts with Human. Virus reactivity reported in scientific literature (PMID: 28373583)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for SARS Nucleocapsid Protein Antibody

  • N protein
  • N structural protein
  • N
  • NC
  • Nucleocapsid protein
  • SARS coronavirus N protein
  • SARS coronavirus nucleocapsid protein
  • SARS CoV N protein
  • SARS CoV nucleocapsid protein
  • SARS CoV
  • SARS N protein
  • SARSCoV N protein
  • SARSCoV nucleocapsid protein
  • SARSCoV
  • Severe acute respiratory syndrome

Background

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for SARS Nucleocapsid Protein Antibody (NB100-56576)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Virus.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(3)
IHC-P
(2)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Virus
(2)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-56576 Applications Species
Channappanavar R, Fett C, Mack M et al. Sex-Based Differences in Susceptibility to Severe Acute Respiratory Syndrome Coronavirus Infection. J. Immunol. Apr 3 2017 [PMID: 28373583] (IHC, Virus) IHC Virus
Fehr AR, Channappanavar R, Jankevicius G et al. The Conserved Coronavirus Macrodomain Promotes Virulence and Suppresses the Innate Immune Response during Severe Acute Respiratory Syndrome Coronavirus Infection. MBio. Dec 13 2016 [PMID: 27965448] (WB, Human) WB Human
Channappanavar R, Fehr AR, Vijay R et al. Dysregulated Type I Interferon and Inflammatory Monocyte-Macrophage Responses Cause Lethal Pneumonia in SARS-CoV-Infected Mice Cell Host Microbe Feb 10 2016 [PMID: 26867177] (IHC-P, Virus) IHC-P Virus
Narayanan K, Ramirez Si, Lokugamage Kg et al. Coronavirus nonstructural protein 1: Common and distinct functions in the regulation of host and viral gene expression Virus Res. 2014 Nov 26 [PMID: 25432065] (WB)

Details:
SARS nucleocapsid Protein antibody used for WB on Vero E6 cells which were either mock-infected (Mock) or infected with wt SARS-CoV (WT) or SARS-CoV-mt (mt), carrying the mutations K164A and H165A in nsp1, at an MOI of 5.
WB
Yasui F, Kohara M, Kitabatake M et al. Phagocytic cells contribute to the antibody-mediated elimination of pulmonary-infected SARS coronavirus. Virology 2014 Apr 01 [PMID: 24725942] (IHC-P, Mouse)

Details:
Lung tissue from SARs-CoV-infected mice,Fig 1C.
IHC-P Mouse
Yoshikawa Tomoki, Hill Terence E, Yoshikawa Naoko et al. Dynamic innate immune responses of human bronchial epithelial cells to severe acute respiratory syndrome-associated coronavirus infection. PLoS One. 2010 [PMID: 20090954] (IHC) IHC
Watts D M, Peters C J, Newman P et al. Evaluation of cotton rats as a model for severe acute respiratory syndrome. Vector Borne Zoonotic Dis. 2008 Jun [PMID: 18447621] (IHC) IHC
Liniger M, Zuniga A, Tamin A et al. Induction of neutralising antibodies and cellular immune responses against SARS coronavirus by recombinant measles viruses. Vaccine. 2008 Apr 16 [PMID: 18346823]
Lokugamage Kumari G, Yoshikawa-Iwata Naoko, Ito Naoto et al. Chimeric coronavirus-like particles carrying severe acute respiratory syndrome coronavirus (SCoV) S protein protect mice against challenge with SCoV. Vaccine. 2008 Feb 6 [PMID: 18191004] (WB) WB
Mossel EC, Wang J, Jeffers S et al. SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells. Virology. 2008 Mar 1 [PMID: 18022664]
Show All 13 Publications.

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Bioinformatics

Gene Symbol N
Uniprot