Recombinant Human RYBP/DEDAF His Protein

SDS-Page: Recombinant Human RYBP/DEDAF Protein [NBP1-98929] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human RYBP/DEDAF His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-228 of Human RYBP/DEDAF
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTMGDK KSPTRPKRQA KPAADEGFWD CSVCTFRNSA EAFKCSICDV RKGTSTRKPR INSQLVAQQV AQQYATPPPP KKEKKEKVEK QDKEKPEKDK EISPSVTKKN TNKKTKPKSD ILKDPPSEAN SIQSANATTK TSETNHTSRP RLKNVDRSTA QQLAVTVGNV TVIITDFKEK TRSSSTSSST VTSSAGSEQQ NQSSSGSEST DKGSSRSSTP KGDMSAVNDE SF

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: RYBP
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 27.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT, 100 mM NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human RYBP/DEDAF His Protein

• AAP1
• APAP-1
• Apoptin-associating protein 1
• Death effector domain-associated factor
• DEDAFYY1 and E4TF1-associated factor 1
• RING1 and YY1 binding protein
• RING1 and YY1-binding protein
• ring1 interactor RYBP
• YEAF1DED-associated factor
• YY1 and E4TF1 associated factor 1

Background

RYBP (RING1- and YY1-binding protein) is a member of the polycomb group (PcG). This protein interacts with MDM2 and decreases MDM2-mediated p53 ubiquitination, leading to stabilization of p53 and an increase in p53 activity. RYBP induces cell-cycle arrest and is involved in the p53 response to DNA damage. RYBP has been shown to interact with RING1, YY1, Caspase 10, E2F3, E2F2, Mdm2, Abl gene and CBX2. Recombinant human RYBP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RYBP