RPS24 Overexpression Lysate (Native)

Images

 
Western Blot: RPS24 Overexpression Lysate (Adult Normal) [NBL1-15566] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RPS24.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RPS24 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210033 and based on accession number NM_033022. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ribosomal protein S24 (RPS24), transcript variant a, mRNA.
Gene
RPS24

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
14.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RPS24

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RPS24 Overexpression Lysate (Native)

  • DBA3,40S ribosomal protein S24
  • DKFZp686N1586
  • ribosomal protein S24

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S24E family of ribosomal proteins. It is located in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Mutations in this gene result in Diamond-Blackfan anemia. [provided by RefSeq]. Transcript Variant: This variant (a) uses an alternate splice pattern in the 3' coding region, compared to variant d. The resulting protein (isoform a) has a shorter and distinct C-terminus, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for RPS24 Lysate (NBL1-15566) (0)

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Video Protocols

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Bioinformatics

Gene Symbol RPS24