RNF180 Antibody

Western Blot: RNF180 Antibody [NBP2-20180] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:2000.

Immunohistochemistry-Paraffin: RNF180 Antibody [NBP2-20180] - Analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.

Product Discontinued

RNF180 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RNF180. The exact sequence is proprietary.
• Localization: Membrane; Single-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF180
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%), Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for RNF180 Antibody

• E3 ubiquitin-protein ligase RNF180
• EC 6.3.2.-
• MGC120328
• ring finger protein 180MGC120326

Background

E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RNF180
• Uniprot:
  • Q86T96(Human)