RNF180 Antibody

Immunohistochemistry-Paraffin: RNF180 Antibody [NBP1-86127] - Staining of human cerebral cortex shows strong positivity in neuropil.

Immunohistochemistry-Paraffin: RNF180 Antibody [NBP1-86127] - Staining of human endometrium shows weak membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: RNF180 Antibody [NBP1-86127] - Staining of human kidney shows weak to moderate membranous positivity.

Immunohistochemistry-Paraffin: RNF180 Antibody [NBP1-86127] - Staining of human placenta shows low positivity in trophoblastic cells as expected.

Immunohistochemistry-Paraffin: RNF180 Antibody [NBP1-86127] - Staining of human parathyroid gland shows moderate membranous positivity in glandular cells.

Product Discontinued

RNF180 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EKLTLLPTLYEIHSKTTAYSRLNETQPIDLSGLPLQSSKNSYSFQNPSSFDPSMLLQRFSVAPHETQTQRGGEFQCGLEAASVYSDHTNTNNLTFLMDLPSAGRSMPEASDQEEHLSPLDFLHSAN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF180
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RNF180 Antibody

• E3 ubiquitin-protein ligase RNF180
• EC 6.3.2.-
• MGC120328
• ring finger protein 180MGC120326

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF180 Antibody (NBP1-86127)(2)

Publications using NBP1-86127

• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]
• Zhang E, Tang H, Guan Y et al. Overexpression of RNF180 Inhibits Progression and Metastasis by Epithelial Mesenchymal Transformation and Immune Infiltration in Renal Cancer Research Square 2022-02-16 (IF/IHC, Human)

Bioinformatics

• Gene Symbol: RNF180