RIC8A Antibody

Western Blot: RIC8A Antibody [NB100-1473] - Analysis of RIC8A in human brain lysate (RIPA buffer, 35ug total protein per lane) using this antibody at 1ug/ml. Primary incubated for 1 hour. Detected using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RIC8A Antibody Summary

• Description: Novus Biologicals Goat RIC8A Antibody (NB100-1473) is a polyclonal antibody validated for use in WB and ELISA. Anti-RIC8A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CLSSDPDSDPD corresponding to C-Terminus according to NP_068751.4.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RIC8A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human brain lysates (calculated MW of 60.4 kDa band according to NP_068751.4).
• Control:

  RIC8A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RIC8A Antibody

• RIC8
• RIC8A resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
• synembryn
• synembryn-A

Background

RIC8 is a likely orthalog of mouse Synembryn. Synembryns are proteins that positively regulate synaptic transmission. They are actively required to maintain proper activity of the Go to Gq G-protein signalling network, which regulates neurotransmitter secretion in Caenorhabditis elegans by controlling the production and consumption of diacylglycerol. In addition to its role in the adult nervous system, synembryn is required to regulate a subset of centrosome movements in the early C. elegans embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RIC8A Antibody (NB100-1473)(1)

Publication using NB100-1473

• Klattenhoff C, Montecino M, Soto X et al. Human brain synembryn interacts with Gsalpha and Gqalpha and is translocated to the plasma membrane in response to isoproterenol and carbachol. J Cell Physiol 2003-05-01 [PMID: 12652642]

Bioinformatics

• Gene Symbol: RIC8A
• Entrez:
  • 60626(Human)
  • 101489(Mouse)
• Uniprot:
  • NP_068751.(Human)