| Immunogen | Synthetic peptide with sequence CEMSLISQSTRLNSYS, representing the C Terminus of the human protein according to NP_067647.1 |
| Epitope | C Terminus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | RXFP1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments gave bands at approx 100kDa and 35kDa in Human Colon and Heart lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 87kDa according to NP_067647.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | Tris (pH 7.3), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | RXFP1 |
| Entrez |
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| Uniprot |
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