![Western Blot: REEP2 Antibody (S326D-29) [NBP2-59314] - Analysis of Rat Brain showing detection of ~30 kDa REEP1-2 protein using Mouse Anti-REEP1-2 Monoclonal Antibody, Clone S326D-29. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug . Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-REEP1-2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~30 kDa. Other Band(s): ~22 kDa.](http://images.novusbio.com/fullsize2/REEP2-Antibody-(S326D-29)-Western-Blot-NBP2-59314-img0004.jpg "Western Blot: REEP2 Antibody (S326D-29) [NBP2-59314] - Analysis of Rat Brain showing detection of ~30 kDa REEP1-2 protein using Mouse Anti-REEP1-2 Monoclonal Antibody, Clone S326D-29. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug . Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-REEP1-2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~30 kDa. Other Band(s): ~22 kDa.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | S326D-29 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Fusion protein amino acids 111-254 (Cytoplasmic C-terminus) of mouse REEP2. |
| Localization | Mitochondrion , Mitochondrion Membrane , Endoplasmic Reticulum , Endoplasmic Reticulum Membrane |
| Specificity | Detects 30kDa. Cross-reacts with REEP1. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | REEP2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.1% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | REEP2 |
![Immunocytochemistry/Immunofluorescence: REEP2 Antibody (S326D-29) [NBP2-59314] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-REEP1/2 Monoclonal Antibody at 1:50 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Membrane, Endoplasmic Reticulum, Endoplasmic Reticulum Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize2/REEP2-Antibody-(S326D-29)-Immunocytochemistry-Immunofluorescence-NBP2-59314-img0002.jpg "Immunocytochemistry/Immunofluorescence: REEP2 Antibody (S326D-29) [NBP2-59314] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-REEP1/2 Monoclonal Antibody at 1:50 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Membrane, Endoplasmic Reticulum, Endoplasmic Reticulum Membrane. Magnification: 60X.")
![Slit2 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Slit2 [Unconjugated]")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-(MG1)-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")